Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders

Abstract The lung is inhabited by a diverse microbiome that originates from the oropharynx by a mechanism of micro-aspiration. Its bacterial biomass is usually low; however, this condition shifts in lung cancer (LC), chronic obstructive pulmonary disease (COPD) and interstitial lung disease (ILD). T...

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Autores principales:	Susana Seixas, Allison R. Kolbe, Sílvia Gomes, Maria Sucena, Catarina Sousa, Luís Vaz Rodrigues, Gilberto Teixeira, Paula Pinto, Tiago Tavares de Abreu, Cristina Bárbara, Júlio Semedo, Leonor Mota, Ana Sofia Carvalho, Rune Matthiesen, Patrícia Isabel Marques, Marcos Pérez-Losada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd1753bc1e0044919a36c1fb863cf86d
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spelling	oai:doaj.org-article:fd1753bc1e0044919a36c1fb863cf86d2021-12-02T17:55:09ZComparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders10.1038/s41598-021-94468-y2045-2322https://doaj.org/article/fd1753bc1e0044919a36c1fb863cf86d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94468-yhttps://doaj.org/toc/2045-2322Abstract The lung is inhabited by a diverse microbiome that originates from the oropharynx by a mechanism of micro-aspiration. Its bacterial biomass is usually low; however, this condition shifts in lung cancer (LC), chronic obstructive pulmonary disease (COPD) and interstitial lung disease (ILD). These chronic lung disorders (CLD) may coexist in the same patient as comorbidities and share common risk factors, among which the microbiome is included. We characterized the microbiome of 106 bronchoalveolar lavages. Samples were initially subdivided into cancer and non-cancer and high-throughput sequenced for the 16S rRNA gene. Additionally, we used a cohort of 25 CLD patients where crossed comorbidities were excluded. Firmicutes, Proteobacteria and Bacteroidetes were the most prevalent phyla independently of the analyzed group. Streptococcus and Prevotella were associated with LC and Haemophilus was enhanced in COPD versus ILD. Although no significant discrepancies in microbial diversity were observed between cancer and non-cancer samples, statistical tests suggested a gradient across CLD where COPD and ILD displayed the highest and lowest alpha diversities, respectively. Moreover, COPD and ILD were separated in two clusters by the unweighted UniFrac distance (P value = 0.0068). Our results support the association of Streptoccocus and Prevotella with LC and of Haemophilus with COPD, and advocate for specific CLD signatures.Susana SeixasAllison R. KolbeSílvia GomesMaria SucenaCatarina SousaLuís Vaz RodriguesGilberto TeixeiraPaula PintoTiago Tavares de AbreuCristina BárbaraJúlio SemedoLeonor MotaAna Sofia CarvalhoRune MatthiesenPatrícia Isabel MarquesMarcos Pérez-LosadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susana Seixas Allison R. Kolbe Sílvia Gomes Maria Sucena Catarina Sousa Luís Vaz Rodrigues Gilberto Teixeira Paula Pinto Tiago Tavares de Abreu Cristina Bárbara Júlio Semedo Leonor Mota Ana Sofia Carvalho Rune Matthiesen Patrícia Isabel Marques Marcos Pérez-Losada Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders
description	Abstract The lung is inhabited by a diverse microbiome that originates from the oropharynx by a mechanism of micro-aspiration. Its bacterial biomass is usually low; however, this condition shifts in lung cancer (LC), chronic obstructive pulmonary disease (COPD) and interstitial lung disease (ILD). These chronic lung disorders (CLD) may coexist in the same patient as comorbidities and share common risk factors, among which the microbiome is included. We characterized the microbiome of 106 bronchoalveolar lavages. Samples were initially subdivided into cancer and non-cancer and high-throughput sequenced for the 16S rRNA gene. Additionally, we used a cohort of 25 CLD patients where crossed comorbidities were excluded. Firmicutes, Proteobacteria and Bacteroidetes were the most prevalent phyla independently of the analyzed group. Streptococcus and Prevotella were associated with LC and Haemophilus was enhanced in COPD versus ILD. Although no significant discrepancies in microbial diversity were observed between cancer and non-cancer samples, statistical tests suggested a gradient across CLD where COPD and ILD displayed the highest and lowest alpha diversities, respectively. Moreover, COPD and ILD were separated in two clusters by the unweighted UniFrac distance (P value = 0.0068). Our results support the association of Streptoccocus and Prevotella with LC and of Haemophilus with COPD, and advocate for specific CLD signatures.
format	article
author	Susana Seixas Allison R. Kolbe Sílvia Gomes Maria Sucena Catarina Sousa Luís Vaz Rodrigues Gilberto Teixeira Paula Pinto Tiago Tavares de Abreu Cristina Bárbara Júlio Semedo Leonor Mota Ana Sofia Carvalho Rune Matthiesen Patrícia Isabel Marques Marcos Pérez-Losada
author_facet	Susana Seixas Allison R. Kolbe Sílvia Gomes Maria Sucena Catarina Sousa Luís Vaz Rodrigues Gilberto Teixeira Paula Pinto Tiago Tavares de Abreu Cristina Bárbara Júlio Semedo Leonor Mota Ana Sofia Carvalho Rune Matthiesen Patrícia Isabel Marques Marcos Pérez-Losada
author_sort	Susana Seixas
title	Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders
title_short	Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders
title_full	Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders
title_fullStr	Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders
title_full_unstemmed	Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders
title_sort	comparative analysis of the bronchoalveolar microbiome in portuguese patients with different chronic lung disorders
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fd1753bc1e0044919a36c1fb863cf86d
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Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders

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