Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

Abstract The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the spindle organisation in mitotic cell...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rinyaporn Phengchat, Hideaki Takata, Susumu Uchiyama, Kiichi Fukui
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd17d657b65a4c56806f58d83f68ae96
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fd17d657b65a4c56806f58d83f68ae96
record_format	dspace
spelling	oai:doaj.org-article:fd17d657b65a4c56806f58d83f68ae962021-12-02T11:52:32ZCalcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore10.1038/s41598-017-07777-62045-2322https://doaj.org/article/fd17d657b65a4c56806f58d83f68ae962017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07777-6https://doaj.org/toc/2045-2322Abstract The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the spindle organisation in mitotic cells remains controversial. Here, we investigated the role of Ca2+ on kinetochore fibres in living cells. We found that depletion of Ca2+ during mitosis reduced kinetochore fibre stability. Reduction of kinetochore fibre stability was not due to direct inhibition of microtubule polymerisation by Ca2+-depletion but due to elimination of one dynamic component of kinetochore, CENP-F from the kinetochore. This compromised the attachment of kinetochore fibres to the kinetochore which possibly causes mitotic defects induced by the depletion of Ca2+.Rinyaporn PhengchatHideaki TakataSusumu UchiyamaKiichi FukuiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rinyaporn Phengchat Hideaki Takata Susumu Uchiyama Kiichi Fukui Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
description	Abstract The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the spindle organisation in mitotic cells remains controversial. Here, we investigated the role of Ca2+ on kinetochore fibres in living cells. We found that depletion of Ca2+ during mitosis reduced kinetochore fibre stability. Reduction of kinetochore fibre stability was not due to direct inhibition of microtubule polymerisation by Ca2+-depletion but due to elimination of one dynamic component of kinetochore, CENP-F from the kinetochore. This compromised the attachment of kinetochore fibres to the kinetochore which possibly causes mitotic defects induced by the depletion of Ca2+.
format	article
author	Rinyaporn Phengchat Hideaki Takata Susumu Uchiyama Kiichi Fukui
author_facet	Rinyaporn Phengchat Hideaki Takata Susumu Uchiyama Kiichi Fukui
author_sort	Rinyaporn Phengchat
title	Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
title_short	Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
title_full	Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
title_fullStr	Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
title_full_unstemmed	Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
title_sort	calcium depletion destabilises kinetochore fibres by the removal of cenp-f from the kinetochore
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fd17d657b65a4c56806f58d83f68ae96
work_keys_str_mv	AT rinyapornphengchat calciumdepletiondestabiliseskinetochorefibresbytheremovalofcenpffromthekinetochore AT hideakitakata calciumdepletiondestabiliseskinetochorefibresbytheremovalofcenpffromthekinetochore AT susumuuchiyama calciumdepletiondestabiliseskinetochorefibresbytheremovalofcenpffromthekinetochore AT kiichifukui calciumdepletiondestabiliseskinetochorefibresbytheremovalofcenpffromthekinetochore
_version_	1718395023907618816

Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

Ejemplares similares