Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.

Transmissible spongiform encephalopathies (TSEs), including scrapie in sheep (Ovis aries), are fatal neurodegenerative diseases caused by the misfolding of the cellular prion protein (PrP(C)) into a â-rich conformer (PrP(Sc)) that accumulates into higher-order structures in the brain and other tissu...

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Autores principales:	Catherine E Vrentas, Justin J Greenlee, Trudy L Tatum, Eric M Nicholson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd1c0a5342bb469db4c04cd6110afb0c
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spelling	oai:doaj.org-article:fd1c0a5342bb469db4c04cd6110afb0c2021-11-18T07:08:44ZRelationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.1932-620310.1371/journal.pone.0043060https://doaj.org/article/fd1c0a5342bb469db4c04cd6110afb0c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22916207/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Transmissible spongiform encephalopathies (TSEs), including scrapie in sheep (Ovis aries), are fatal neurodegenerative diseases caused by the misfolding of the cellular prion protein (PrP(C)) into a â-rich conformer (PrP(Sc)) that accumulates into higher-order structures in the brain and other tissues. Distinct strains of TSEs exist, characterized by different pathologic profiles upon passage into rodents and representing distinct conformations of PrP(Sc). One biochemical method of distinguishing strains is the stability of PrP(Sc) as determined by unfolding in guanidine hydrochloride (GdnHCl), which is tightly and positively correlated with the incubation time of disease upon passage into mice. Here, we utilize a rapid, protease-free version of the stability assay to characterize naturally occurring scrapie samples, including a fast-acting scrapie inoculum for which incubation time is highly dependent on the amino acid at codon 136 of the prion protein. We utilize the stability methodology to identify the presence of two distinct isolates in the inoculum, and compare isolate properties to those of a host-stabilized reference scrapie isolate (NADC 13-7) in order to assess the stability/incubation time correlation in a natural host system. We demonstrate the utility of the stability methodology in characterizing TSE isolates throughout serial passage in livestock, which is applicable to a range of natural host systems, including strains of bovine spongiform encephalopathy and chronic wasting disease.Catherine E VrentasJustin J GreenleeTrudy L TatumEric M NicholsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43060 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Catherine E Vrentas Justin J Greenlee Trudy L Tatum Eric M Nicholson Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.
description	Transmissible spongiform encephalopathies (TSEs), including scrapie in sheep (Ovis aries), are fatal neurodegenerative diseases caused by the misfolding of the cellular prion protein (PrP(C)) into a â-rich conformer (PrP(Sc)) that accumulates into higher-order structures in the brain and other tissues. Distinct strains of TSEs exist, characterized by different pathologic profiles upon passage into rodents and representing distinct conformations of PrP(Sc). One biochemical method of distinguishing strains is the stability of PrP(Sc) as determined by unfolding in guanidine hydrochloride (GdnHCl), which is tightly and positively correlated with the incubation time of disease upon passage into mice. Here, we utilize a rapid, protease-free version of the stability assay to characterize naturally occurring scrapie samples, including a fast-acting scrapie inoculum for which incubation time is highly dependent on the amino acid at codon 136 of the prion protein. We utilize the stability methodology to identify the presence of two distinct isolates in the inoculum, and compare isolate properties to those of a host-stabilized reference scrapie isolate (NADC 13-7) in order to assess the stability/incubation time correlation in a natural host system. We demonstrate the utility of the stability methodology in characterizing TSE isolates throughout serial passage in livestock, which is applicable to a range of natural host systems, including strains of bovine spongiform encephalopathy and chronic wasting disease.
format	article
author	Catherine E Vrentas Justin J Greenlee Trudy L Tatum Eric M Nicholson
author_facet	Catherine E Vrentas Justin J Greenlee Trudy L Tatum Eric M Nicholson
author_sort	Catherine E Vrentas
title	Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.
title_short	Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.
title_full	Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.
title_fullStr	Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.
title_full_unstemmed	Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.
title_sort	relationships between prpsc stability and incubation time for united states scrapie isolates in a natural host system.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/fd1c0a5342bb469db4c04cd6110afb0c
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Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system.

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