Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.

The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of these complexes during S phase. To determine the...

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Autores principales:	Eric J Foss, Smitha Sripathy, Tonibelle Gatbonton-Schwager, Hyunchang Kwak, Adam H Thiesen, Uyen Lao, Antonio Bedalov
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/fd1f32a2c42947d080d48e22a5e4b7f4
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spelling	oai:doaj.org-article:fd1f32a2c42947d080d48e22a5e4b7f42021-12-02T20:03:04ZChromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.1553-73901553-740410.1371/journal.pgen.1009714https://doaj.org/article/fd1f32a2c42947d080d48e22a5e4b7f42021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009714https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of these complexes during S phase. To determine the degree to which distribution of pre-RC loading alone could account for chromosomal replication patterns, we mapped the binding sites of the Mcm2-7 helicase complex (MCM) in budding yeast, fission yeast, mouse and humans. We observed similar individual MCM double-hexamer (DH) footprints across the species, but notable differences in their distribution: Footprints in budding yeast were more sharply focused compared to the other three organisms, consistent with the relative sequence specificity of replication origins in S. cerevisiae. Nonetheless, with some clear exceptions, most notably the inactive X-chromosome, much of the fluctuation in replication timing along the chromosomes in all four organisms reflected uneven chromosomal distribution of pre-replication complexes.Eric J FossSmitha SripathyTonibelle Gatbonton-SchwagerHyunchang KwakAdam H ThiesenUyen LaoAntonio BedalovPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009714 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Eric J Foss Smitha Sripathy Tonibelle Gatbonton-Schwager Hyunchang Kwak Adam H Thiesen Uyen Lao Antonio Bedalov Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.
description	The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of these complexes during S phase. To determine the degree to which distribution of pre-RC loading alone could account for chromosomal replication patterns, we mapped the binding sites of the Mcm2-7 helicase complex (MCM) in budding yeast, fission yeast, mouse and humans. We observed similar individual MCM double-hexamer (DH) footprints across the species, but notable differences in their distribution: Footprints in budding yeast were more sharply focused compared to the other three organisms, consistent with the relative sequence specificity of replication origins in S. cerevisiae. Nonetheless, with some clear exceptions, most notably the inactive X-chromosome, much of the fluctuation in replication timing along the chromosomes in all four organisms reflected uneven chromosomal distribution of pre-replication complexes.
format	article
author	Eric J Foss Smitha Sripathy Tonibelle Gatbonton-Schwager Hyunchang Kwak Adam H Thiesen Uyen Lao Antonio Bedalov
author_facet	Eric J Foss Smitha Sripathy Tonibelle Gatbonton-Schwager Hyunchang Kwak Adam H Thiesen Uyen Lao Antonio Bedalov
author_sort	Eric J Foss
title	Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.
title_short	Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.
title_full	Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.
title_fullStr	Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.
title_full_unstemmed	Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.
title_sort	chromosomal mcm2-7 distribution and the genome replication program in species from yeast to humans.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/fd1f32a2c42947d080d48e22a5e4b7f4
work_keys_str_mv	AT ericjfoss chromosomalmcm27distributionandthegenomereplicationprograminspeciesfromyeasttohumans AT smithasripathy chromosomalmcm27distributionandthegenomereplicationprograminspeciesfromyeasttohumans AT tonibellegatbontonschwager chromosomalmcm27distributionandthegenomereplicationprograminspeciesfromyeasttohumans AT hyunchangkwak chromosomalmcm27distributionandthegenomereplicationprograminspeciesfromyeasttohumans AT adamhthiesen chromosomalmcm27distributionandthegenomereplicationprograminspeciesfromyeasttohumans AT uyenlao chromosomalmcm27distributionandthegenomereplicationprograminspeciesfromyeasttohumans AT antoniobedalov chromosomalmcm27distributionandthegenomereplicationprograminspeciesfromyeasttohumans
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Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.

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