Selective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination

Urine-based biomarkers have shown suitable diagnostic potential for prostate cancer (PCa) detection. Yet, until now, prostatic massage remains required prior to urine sampling. Here, we test a potential diagnostic approach using voided urine collected without prior digital rectal examination (DRE)....

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Autores principales:	Kit Man Chan, Jonathan M. Gleadle, Philip A. Gregory, Caroline A. Phillips, Hanieh Safizadeh Shirazi, Amelia Whiteley, Jordan Li, Krasimir Vasilev, Melanie MacGregor
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	urine prostate cancer cancer detection microfluidic nanotechnologies biosensors Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/fd2092a4775b49d1924cca1c5f85c926
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spelling	oai:doaj.org-article:fd2092a4775b49d1924cca1c5f85c9262021-11-11T15:34:25ZSelective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination10.3390/cancers132155442072-6694https://doaj.org/article/fd2092a4775b49d1924cca1c5f85c9262021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5544https://doaj.org/toc/2072-6694Urine-based biomarkers have shown suitable diagnostic potential for prostate cancer (PCa) detection. Yet, until now, prostatic massage remains required prior to urine sampling. Here, we test a potential diagnostic approach using voided urine collected without prior digital rectal examination (DRE). In this study, we evaluated the diagnostic performance of a microfluidic-based platform that combines the principle of photodynamic diagnostic with immunocapture for the detection of PCa cells. The functionality and sensitivity of this platform were validated using both cultured cells and PCa patient urine samples. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) demonstrated this platform had a detection limit of fewer than 10 cells per 60 µL and successfully validated the presence of a PCa biomarker in the urine of cancer patients without prior DRE. This biosensing platform exhibits a sensitivity of 72.4% and a specificity of 71.4%, in suitable agreement with qRT-PCR data. The results of this study constitute a stepping stone in the future development of noninvasive prostate cancer diagnostic technologies that do not require DRE.Kit Man ChanJonathan M. GleadlePhilip A. GregoryCaroline A. PhillipsHanieh Safizadeh ShiraziAmelia WhiteleyJordan LiKrasimir VasilevMelanie MacGregorMDPI AGarticleurineprostate cancercancer detectionmicrofluidicnanotechnologiesbiosensorsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5544, p 5544 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	urine prostate cancer cancer detection microfluidic nanotechnologies biosensors Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	urine prostate cancer cancer detection microfluidic nanotechnologies biosensors Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Kit Man Chan Jonathan M. Gleadle Philip A. Gregory Caroline A. Phillips Hanieh Safizadeh Shirazi Amelia Whiteley Jordan Li Krasimir Vasilev Melanie MacGregor Selective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination
description	Urine-based biomarkers have shown suitable diagnostic potential for prostate cancer (PCa) detection. Yet, until now, prostatic massage remains required prior to urine sampling. Here, we test a potential diagnostic approach using voided urine collected without prior digital rectal examination (DRE). In this study, we evaluated the diagnostic performance of a microfluidic-based platform that combines the principle of photodynamic diagnostic with immunocapture for the detection of PCa cells. The functionality and sensitivity of this platform were validated using both cultured cells and PCa patient urine samples. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) demonstrated this platform had a detection limit of fewer than 10 cells per 60 µL and successfully validated the presence of a PCa biomarker in the urine of cancer patients without prior DRE. This biosensing platform exhibits a sensitivity of 72.4% and a specificity of 71.4%, in suitable agreement with qRT-PCR data. The results of this study constitute a stepping stone in the future development of noninvasive prostate cancer diagnostic technologies that do not require DRE.
format	article
author	Kit Man Chan Jonathan M. Gleadle Philip A. Gregory Caroline A. Phillips Hanieh Safizadeh Shirazi Amelia Whiteley Jordan Li Krasimir Vasilev Melanie MacGregor
author_facet	Kit Man Chan Jonathan M. Gleadle Philip A. Gregory Caroline A. Phillips Hanieh Safizadeh Shirazi Amelia Whiteley Jordan Li Krasimir Vasilev Melanie MacGregor
author_sort	Kit Man Chan
title	Selective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination
title_short	Selective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination
title_full	Selective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination
title_fullStr	Selective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination
title_full_unstemmed	Selective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination
title_sort	selective microfluidic capture and detection of prostate cancer cells from urine without digital rectal examination
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fd2092a4775b49d1924cca1c5f85c926
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Selective Microfluidic Capture and Detection of Prostate Cancer Cells from Urine without Digital Rectal Examination

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