Use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial

Background: Exposure to pesticides has been associated with oxidative stress in animals and humans. Previously, we showed that an organic food intervention reduced pesticide exposure and oxidative damage (OD) biomarkers over time; however associated metabolic changes are not fully understood yet. Ob...

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Autores principales:	Corina Konstantinou, Stephanie Gaengler, Stavros Oikonomou, Thibaut Delplancke, Pantelis Charisiadis, Konstantinos C. Makris
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Organic food Metabolomics Pesticides Cluster randomized trial Oxidative stress Oxidative damage Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/fd25159fffc54f5d9ab01739f9b609bf
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spelling	oai:doaj.org-article:fd25159fffc54f5d9ab01739f9b609bf2021-12-02T04:58:59ZUse of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial0160-412010.1016/j.envint.2021.107008https://doaj.org/article/fd25159fffc54f5d9ab01739f9b609bf2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0160412021006334https://doaj.org/toc/0160-4120Background: Exposure to pesticides has been associated with oxidative stress in animals and humans. Previously, we showed that an organic food intervention reduced pesticide exposure and oxidative damage (OD) biomarkers over time; however associated metabolic changes are not fully understood yet. Objectives: We assessed perturbations of the urine metabolome in response to an organic food intervention for children and its association with pesticides biomarkers [3-phenoxybenzoic acid (3-PBA) and 6-chloronicotinic acid (6-CN)]. We also evaluated the molecular signatures of metabolites associated with biomarkers of OD (8-iso-PGF2a and 8-OHdG) and related biological pathways. Methods: We used data from the ORGANIKO LIFE + trial (NCT02998203), a cluster-randomized cross-over trial conducted among primary school children in Cyprus. Participants (n = 149) were asked to follow an organic food intervention for 40 days and their usual food habits for another 40 days, providing up to six first morning urine samples (>850 samples in total). Untargeted GC–MS metabolomics analysis was performed. Metabolites with RSD ≤ 20% and D-ratio ≤ 50% were retained for analysis. Associations were examined using mixed-effect regression models and corrected for false-discovery rate of 0.05. Pathway analysis followed. Results: Following strict quality checks, 156 features remained out of a total of 610. D-glucose was associated with the organic food intervention (β = −0.23, 95% CI: −0.37,−0.10), aminomalonic acid showed a time-dependent increase during the intervention period (βint = 0.012; 95% CI:0.002, 0.022) and was associated with the two OD biomarkers (β = −0.27, 95% CI:−0.34,−0.20 for 8-iso-PGF2a and β = 0.19, 95% CI:0.11,0.28 for 8-OHdG) and uric acid with 8-OHdG (β = 0.19, 95% CI:0.11,0.26). Metabolites were involved in pathways such as the starch and sucrose metabolism and pentose and glucuronate interconversions. Discussion: This is the first metabolomics study providing evidence of differential expression of metabolites by an organic food intervention, corroborating the reduction in biomarkers of OD. Further mechanistic evidence is warranted to better understand the biological plausibility of an organic food treatment on children’s health outcomes.Corina KonstantinouStephanie GaenglerStavros OikonomouThibaut DelplanckePantelis CharisiadisKonstantinos C. MakrisElsevierarticleOrganic foodMetabolomicsPesticidesCluster randomized trialOxidative stressOxidative damageEnvironmental sciencesGE1-350ENEnvironment International, Vol 158, Iss , Pp 107008- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Organic food Metabolomics Pesticides Cluster randomized trial Oxidative stress Oxidative damage Environmental sciences GE1-350
spellingShingle	Organic food Metabolomics Pesticides Cluster randomized trial Oxidative stress Oxidative damage Environmental sciences GE1-350 Corina Konstantinou Stephanie Gaengler Stavros Oikonomou Thibaut Delplancke Pantelis Charisiadis Konstantinos C. Makris Use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial
description	Background: Exposure to pesticides has been associated with oxidative stress in animals and humans. Previously, we showed that an organic food intervention reduced pesticide exposure and oxidative damage (OD) biomarkers over time; however associated metabolic changes are not fully understood yet. Objectives: We assessed perturbations of the urine metabolome in response to an organic food intervention for children and its association with pesticides biomarkers [3-phenoxybenzoic acid (3-PBA) and 6-chloronicotinic acid (6-CN)]. We also evaluated the molecular signatures of metabolites associated with biomarkers of OD (8-iso-PGF2a and 8-OHdG) and related biological pathways. Methods: We used data from the ORGANIKO LIFE + trial (NCT02998203), a cluster-randomized cross-over trial conducted among primary school children in Cyprus. Participants (n = 149) were asked to follow an organic food intervention for 40 days and their usual food habits for another 40 days, providing up to six first morning urine samples (>850 samples in total). Untargeted GC–MS metabolomics analysis was performed. Metabolites with RSD ≤ 20% and D-ratio ≤ 50% were retained for analysis. Associations were examined using mixed-effect regression models and corrected for false-discovery rate of 0.05. Pathway analysis followed. Results: Following strict quality checks, 156 features remained out of a total of 610. D-glucose was associated with the organic food intervention (β = −0.23, 95% CI: −0.37,−0.10), aminomalonic acid showed a time-dependent increase during the intervention period (βint = 0.012; 95% CI:0.002, 0.022) and was associated with the two OD biomarkers (β = −0.27, 95% CI:−0.34,−0.20 for 8-iso-PGF2a and β = 0.19, 95% CI:0.11,0.28 for 8-OHdG) and uric acid with 8-OHdG (β = 0.19, 95% CI:0.11,0.26). Metabolites were involved in pathways such as the starch and sucrose metabolism and pentose and glucuronate interconversions. Discussion: This is the first metabolomics study providing evidence of differential expression of metabolites by an organic food intervention, corroborating the reduction in biomarkers of OD. Further mechanistic evidence is warranted to better understand the biological plausibility of an organic food treatment on children’s health outcomes.
format	article
author	Corina Konstantinou Stephanie Gaengler Stavros Oikonomou Thibaut Delplancke Pantelis Charisiadis Konstantinos C. Makris
author_facet	Corina Konstantinou Stephanie Gaengler Stavros Oikonomou Thibaut Delplancke Pantelis Charisiadis Konstantinos C. Makris
author_sort	Corina Konstantinou
title	Use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial
title_short	Use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial
title_full	Use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial
title_fullStr	Use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial
title_full_unstemmed	Use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial
title_sort	use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in cyprus: a cluster-randomized cross-over trial
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/fd25159fffc54f5d9ab01739f9b609bf
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Use of metabolomics in refining the effect of an organic food intervention on biomarkers of exposure to pesticides and biomarkers of oxidative damage in primary school children in Cyprus: A cluster-randomized cross-over trial

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