Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma

Abstract Despite improvement in preoperative imaging, surgical technique, and adjuvant therapy, the prognosis of patients with tongue squamous cell carcinoma (SCC) is still unsatisfactory. The mammalian target of rapamycin (mTOR) play a key role in the regulation of tumor cell proliferation and surv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shau-Hsuan Li, Chih-Yen Chien, Wan-Ting Huang, Sheng-Dean Luo, Yan-Ye Su, Wan-Yu Tien, Ya-Chun Lan, Chang-Han Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd2698bc2bc24049b38305677a64d159
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fd2698bc2bc24049b38305677a64d159
record_format	dspace
spelling	oai:doaj.org-article:fd2698bc2bc24049b38305677a64d1592021-12-02T11:53:10ZPrognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma10.1038/s41598-017-08345-82045-2322https://doaj.org/article/fd2698bc2bc24049b38305677a64d1592017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08345-8https://doaj.org/toc/2045-2322Abstract Despite improvement in preoperative imaging, surgical technique, and adjuvant therapy, the prognosis of patients with tongue squamous cell carcinoma (SCC) is still unsatisfactory. The mammalian target of rapamycin (mTOR) play a key role in the regulation of tumor cell proliferation and survival. However, the significance of mTOR on the prognosis of tongue SCC remains largely undefined. In the present study, immunohistochemistry was performed to evaluate the expression of phosphorylated mTOR (p-mTOR) in 160 surgically resected tongue SCC, and correlated with survival. Univariate analysis revealed that p-mTOR overexpression (P = 0.006) was associated with inferior overall survival. In multivariate comparison, p-mTOR overexpression (P = 0.002, hazard ratio = 2.082) remained independently associated with worse overall survival. In vitro study, tongue cancer cells treated with everolimus, the specific mTOR inhibitor, or transfected with mTOR-mediated siRNAs dramatically attenuated the abilities of cell proliferation by MTT and BrdU assays. In 4-NQO-induced tongue cancer murine model, mTOR inhibitors significantly decreased the incidence of tongue SCC. In conclusion, p-mTOR overexpression was independently associated with poor prognosis of patients with tongue SCC. In vitro and vivo, mTOR inhibition showed the promising activity in tongue SCC. Our results suggest that inhibition of mTOR signaling pathway may be a novel therapeutic target for tongue SCC.Shau-Hsuan LiChih-Yen ChienWan-Ting HuangSheng-Dean LuoYan-Ye SuWan-Yu TienYa-Chun LanChang-Han ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shau-Hsuan Li Chih-Yen Chien Wan-Ting Huang Sheng-Dean Luo Yan-Ye Su Wan-Yu Tien Ya-Chun Lan Chang-Han Chen Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma
description	Abstract Despite improvement in preoperative imaging, surgical technique, and adjuvant therapy, the prognosis of patients with tongue squamous cell carcinoma (SCC) is still unsatisfactory. The mammalian target of rapamycin (mTOR) play a key role in the regulation of tumor cell proliferation and survival. However, the significance of mTOR on the prognosis of tongue SCC remains largely undefined. In the present study, immunohistochemistry was performed to evaluate the expression of phosphorylated mTOR (p-mTOR) in 160 surgically resected tongue SCC, and correlated with survival. Univariate analysis revealed that p-mTOR overexpression (P = 0.006) was associated with inferior overall survival. In multivariate comparison, p-mTOR overexpression (P = 0.002, hazard ratio = 2.082) remained independently associated with worse overall survival. In vitro study, tongue cancer cells treated with everolimus, the specific mTOR inhibitor, or transfected with mTOR-mediated siRNAs dramatically attenuated the abilities of cell proliferation by MTT and BrdU assays. In 4-NQO-induced tongue cancer murine model, mTOR inhibitors significantly decreased the incidence of tongue SCC. In conclusion, p-mTOR overexpression was independently associated with poor prognosis of patients with tongue SCC. In vitro and vivo, mTOR inhibition showed the promising activity in tongue SCC. Our results suggest that inhibition of mTOR signaling pathway may be a novel therapeutic target for tongue SCC.
format	article
author	Shau-Hsuan Li Chih-Yen Chien Wan-Ting Huang Sheng-Dean Luo Yan-Ye Su Wan-Yu Tien Ya-Chun Lan Chang-Han Chen
author_facet	Shau-Hsuan Li Chih-Yen Chien Wan-Ting Huang Sheng-Dean Luo Yan-Ye Su Wan-Yu Tien Ya-Chun Lan Chang-Han Chen
author_sort	Shau-Hsuan Li
title	Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma
title_short	Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma
title_full	Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma
title_fullStr	Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma
title_full_unstemmed	Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma
title_sort	prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fd2698bc2bc24049b38305677a64d159
work_keys_str_mv	AT shauhsuanli prognosticsignificanceandfunctionofmammaliantargetofrapamycinintonguesquamouscellcarcinoma AT chihyenchien prognosticsignificanceandfunctionofmammaliantargetofrapamycinintonguesquamouscellcarcinoma AT wantinghuang prognosticsignificanceandfunctionofmammaliantargetofrapamycinintonguesquamouscellcarcinoma AT shengdeanluo prognosticsignificanceandfunctionofmammaliantargetofrapamycinintonguesquamouscellcarcinoma AT yanyesu prognosticsignificanceandfunctionofmammaliantargetofrapamycinintonguesquamouscellcarcinoma AT wanyutien prognosticsignificanceandfunctionofmammaliantargetofrapamycinintonguesquamouscellcarcinoma AT yachunlan prognosticsignificanceandfunctionofmammaliantargetofrapamycinintonguesquamouscellcarcinoma AT changhanchen prognosticsignificanceandfunctionofmammaliantargetofrapamycinintonguesquamouscellcarcinoma
_version_	1718394904896339968

Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma

Ejemplares similares