Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties

The use of high throughput sequencing (HTS) for the analysis of Spanish olive trees showing leaf yellowing discoloration, defoliation, and/or decline has provided new insights into the olive viruses present in Spain and has opened discussions about the pros and cons of these technologies for diagnos...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ana Belén Ruiz-García, Celia Canales, Félix Morán, Manuel Ruiz-Torres, Magdalena Herrera-Mármol, Antonio Olmos
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	olive OLYaV OEGV OLV-3 HTS Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fd352c21332741afb6ac9293260c19b7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fd352c21332741afb6ac9293260c19b7
record_format	dspace
spelling	oai:doaj.org-article:fd352c21332741afb6ac9293260c19b72021-11-25T19:13:36ZCharacterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties10.3390/v131122331999-4915https://doaj.org/article/fd352c21332741afb6ac9293260c19b72021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2233https://doaj.org/toc/1999-4915The use of high throughput sequencing (HTS) for the analysis of Spanish olive trees showing leaf yellowing discoloration, defoliation, and/or decline has provided new insights into the olive viruses present in Spain and has opened discussions about the pros and cons of these technologies for diagnostic purposes. In this study, we report for the first time in Spanish orchards the presence of olive leaf yellowing-associated virus (OLYaV), for which the second full coding sequence has been determined. This virus has also been detected in a putative vector, the psyllid <i>Euphyllura olivina</i>. In addition, the presence in Spain of Olea europaea geminivirus (OEGV), recently reported in Italy, has been confirmed, and the full-length sequence of two isolates was obtained by HTS and Sanger sequencing. These results, as well as the detection of other viral sequences related to olive latent virus 3 (OLV-3) and olive viral satellite RNA, raises questions on the biological significance of the findings, about the requirement of standardization on the interpretation of HTS results, and the necessity of additional tests to confirm the relevance of the HTS detection of viral sequences.Ana Belén Ruiz-GarcíaCelia CanalesFélix MoránManuel Ruiz-TorresMagdalena Herrera-MármolAntonio OlmosMDPI AGarticleoliveOLYaVOEGVOLV-3HTSMicrobiologyQR1-502ENViruses, Vol 13, Iss 2233, p 2233 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	olive OLYaV OEGV OLV-3 HTS Microbiology QR1-502
spellingShingle	olive OLYaV OEGV OLV-3 HTS Microbiology QR1-502 Ana Belén Ruiz-García Celia Canales Félix Morán Manuel Ruiz-Torres Magdalena Herrera-Mármol Antonio Olmos Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties
description	The use of high throughput sequencing (HTS) for the analysis of Spanish olive trees showing leaf yellowing discoloration, defoliation, and/or decline has provided new insights into the olive viruses present in Spain and has opened discussions about the pros and cons of these technologies for diagnostic purposes. In this study, we report for the first time in Spanish orchards the presence of olive leaf yellowing-associated virus (OLYaV), for which the second full coding sequence has been determined. This virus has also been detected in a putative vector, the psyllid <i>Euphyllura olivina</i>. In addition, the presence in Spain of Olea europaea geminivirus (OEGV), recently reported in Italy, has been confirmed, and the full-length sequence of two isolates was obtained by HTS and Sanger sequencing. These results, as well as the detection of other viral sequences related to olive latent virus 3 (OLV-3) and olive viral satellite RNA, raises questions on the biological significance of the findings, about the requirement of standardization on the interpretation of HTS results, and the necessity of additional tests to confirm the relevance of the HTS detection of viral sequences.
format	article
author	Ana Belén Ruiz-García Celia Canales Félix Morán Manuel Ruiz-Torres Magdalena Herrera-Mármol Antonio Olmos
author_facet	Ana Belén Ruiz-García Celia Canales Félix Morán Manuel Ruiz-Torres Magdalena Herrera-Mármol Antonio Olmos
author_sort	Ana Belén Ruiz-García
title	Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties
title_short	Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties
title_full	Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties
title_fullStr	Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties
title_full_unstemmed	Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties
title_sort	characterization of spanish olive virome by high throughput sequencing opens new insights and uncertainties
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fd352c21332741afb6ac9293260c19b7
work_keys_str_mv	AT anabelenruizgarcia characterizationofspanisholiveviromebyhighthroughputsequencingopensnewinsightsanduncertainties AT celiacanales characterizationofspanisholiveviromebyhighthroughputsequencingopensnewinsightsanduncertainties AT felixmoran characterizationofspanisholiveviromebyhighthroughputsequencingopensnewinsightsanduncertainties AT manuelruiztorres characterizationofspanisholiveviromebyhighthroughputsequencingopensnewinsightsanduncertainties AT magdalenaherreramarmol characterizationofspanisholiveviromebyhighthroughputsequencingopensnewinsightsanduncertainties AT antonioolmos characterizationofspanisholiveviromebyhighthroughputsequencingopensnewinsightsanduncertainties
_version_	1718410193959649280

Characterization of Spanish Olive Virome by High Throughput Sequencing Opens New Insights and Uncertainties

Ejemplares similares