Peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss

Abstract Objective of experiment was to determine whether oxidative stress (OS) and inflammation altered embryonic loss in dairy cows. Blood samples were collected at days 0, 16, 32 and 60 after timed (AI) from 200 Holstein cows to determine embryonic loss based on interferon-stimulated gene-15 (ISG...

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Autores principales:	Essa Dirandeh, M. A. Sayyar, Z. Ansari-Pirsaraei, H. Deldar, W. W. Thatcher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd3cbd3a7a324d8ea8246fa91bbf8e7a
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spelling	oai:doaj.org-article:fd3cbd3a7a324d8ea8246fa91bbf8e7a2021-12-02T17:41:30ZPeripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss10.1038/s41598-021-91535-22045-2322https://doaj.org/article/fd3cbd3a7a324d8ea8246fa91bbf8e7a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91535-2https://doaj.org/toc/2045-2322Abstract Objective of experiment was to determine whether oxidative stress (OS) and inflammation altered embryonic loss in dairy cows. Blood samples were collected at days 0, 16, 32 and 60 after timed (AI) from 200 Holstein cows to determine embryonic loss based on interferon-stimulated gene-15 (ISG15) mRNA expression (day 16) and ultrasound at day 32 and day 60. Leucocyte expressions of mRNA TLR2, TLR4, TNF-α, IL1B, IL10, STAT3 (inflammation), PTGS2, PTGES (prostaglandin synthesis), and PLA2G4A and ALOX5AP (eicosanoid metabolism) at days 0 and 16 were determined. Plasma redox status for antioxidant enzymatic activities of glutathione peroxidase (GPX), superoxide dismutase (SOD), total antioxidant capacity (TAC), and concentrations of malondialdehyde (MDA) were determined at days 0, 16, 32 and 60. All antioxidant-redox responses were beneficially significant in pregnant cows diagnosed pregnant at day16 and sustained pregnancy to day 60 compared to non-pregnant cows at day16 or pregnant at day16 and lost embryos by days 32 or 60. The leucocyte mRNA expressions of TLR2, TLR4, STAT 3, IL1B, PTGS2, PLA2G4A and ALOX5AP were greater and PTGES was lower at day16 in pregnant cows that lost embryos early (P < 0.05). In conclusion peripheral leucocyte molecular indicators of inflammation and plasma indicators of OS were altered in pregnant cows undergoing embryonic losses compared to cows with a sustained pregnancy.Essa DirandehM. A. SayyarZ. Ansari-PirsaraeiH. DeldarW. W. ThatcherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Essa Dirandeh M. A. Sayyar Z. Ansari-Pirsaraei H. Deldar W. W. Thatcher Peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss
description	Abstract Objective of experiment was to determine whether oxidative stress (OS) and inflammation altered embryonic loss in dairy cows. Blood samples were collected at days 0, 16, 32 and 60 after timed (AI) from 200 Holstein cows to determine embryonic loss based on interferon-stimulated gene-15 (ISG15) mRNA expression (day 16) and ultrasound at day 32 and day 60. Leucocyte expressions of mRNA TLR2, TLR4, TNF-α, IL1B, IL10, STAT3 (inflammation), PTGS2, PTGES (prostaglandin synthesis), and PLA2G4A and ALOX5AP (eicosanoid metabolism) at days 0 and 16 were determined. Plasma redox status for antioxidant enzymatic activities of glutathione peroxidase (GPX), superoxide dismutase (SOD), total antioxidant capacity (TAC), and concentrations of malondialdehyde (MDA) were determined at days 0, 16, 32 and 60. All antioxidant-redox responses were beneficially significant in pregnant cows diagnosed pregnant at day16 and sustained pregnancy to day 60 compared to non-pregnant cows at day16 or pregnant at day16 and lost embryos by days 32 or 60. The leucocyte mRNA expressions of TLR2, TLR4, STAT 3, IL1B, PTGS2, PLA2G4A and ALOX5AP were greater and PTGES was lower at day16 in pregnant cows that lost embryos early (P < 0.05). In conclusion peripheral leucocyte molecular indicators of inflammation and plasma indicators of OS were altered in pregnant cows undergoing embryonic losses compared to cows with a sustained pregnancy.
format	article
author	Essa Dirandeh M. A. Sayyar Z. Ansari-Pirsaraei H. Deldar W. W. Thatcher
author_facet	Essa Dirandeh M. A. Sayyar Z. Ansari-Pirsaraei H. Deldar W. W. Thatcher
author_sort	Essa Dirandeh
title	Peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss
title_short	Peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss
title_full	Peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss
title_fullStr	Peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss
title_full_unstemmed	Peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss
title_sort	peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fd3cbd3a7a324d8ea8246fa91bbf8e7a
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Peripheral leucocyte molecular indicators of inflammation and oxidative stress are altered in dairy cows with embryonic loss

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