Detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance

Adri&aacute;n Mart&iacute;nez-Rivas1,2, Patrick Chinestra3,4, Gilles Favre3,4, S&eacute;bastien Pinaud1, Child&eacute;rick S&eacute;verac1,2, Jean-Charles Faye3,4, Christophe Vieu1,21LAAS-CNRS; Universit&eacute; de Toulouse, Toulouse, France; 2Universi...

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Autores principales:	Adri&aacute;n Mart&iacute;nez-Rivas, Patrick Chinestra, Gilles Favre, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fd46431a857a47b9a95fac2b4e3b9b09
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spelling	oai:doaj.org-article:fd46431a857a47b9a95fac2b4e3b9b092021-12-02T01:08:03ZDetection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance1176-91141178-2013https://doaj.org/article/fd46431a857a47b9a95fac2b4e3b9b092010-09-01T00:00:00Zhttp://www.dovepress.com/detection-of-label-free-cancer-biomarkers-using-nickel-nanoislands-and-a5218https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Adri&aacute;n Mart&iacute;nez-Rivas1,2, Patrick Chinestra3,4, Gilles Favre3,4, S&eacute;bastien Pinaud1, Child&eacute;rick S&eacute;verac1,2, Jean-Charles Faye3,4, Christophe Vieu1,21LAAS-CNRS; Universit&eacute; de Toulouse, Toulouse, France; 2Universit&eacute; de Toulouse, UPS, INSA, INP, ISAE; LAAS; Toulouse, France; 3INSERM U563, Universit&eacute; de Toulouse, CPTP, &ldquo;Signalisation Cellulaire, GTPase Rho et cancers&rdquo;, Toulouse, France; 4Institut Claudius Regaud, Biology Department, Toulouse, FranceAbstract: We present a technique for the label-free detection and recognition of cancer biomarkers using metal nanoislands intended to be integrated in a novel type of nanobiosensor. His-tagged (scFv)-F7N1N2 is the antibody fragment which is directly immobilized, by coordinative bonds, onto ~5 nm nickel islands, then deposited on the surface of a quartz crystal of a quartz crystal microbalance (QCM) to validate the technique. Biomarker GTPase RhoA was investigated because it has been found to be overexpressed in various tumors and because we have recently isolated and characterized a new conformational scFv which selectively recognizes the active form of RhoA. We implemented a surface chemistry involving an antibiofouling coating of polyethylene glycol silane (PEG-silane) (&lt;2 nm thick) and Ni nanoislands to reach a label-free detection of the active antigen conformation of RhoA, at various concentrations. The methodology proposed here proves the viability of the concept by using Ni nanoislands as an anchoring surface layer enabling the detection of a specific conformation of a protein, identified as a potential cancer biomarker. Hence, this novel methodology can be transferred to a nanobiosensor to detect, at lower time consumption and with high sensitivity, specific biomolecules.Keywords: nickel nanoislands, cancer biomarkers, quartz crystal microbalance, PEG-silane, RhoA protein, nanobiosensor Adri&aacute;n Mart&iacute;nez-RivasPatrick ChinestraGilles Favreet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 661-668 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Adri&aacute;n Mart&iacute;nez-Rivas Patrick Chinestra Gilles Favre et al Detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance
description	Adri&aacute;n Mart&iacute;nez-Rivas1,2, Patrick Chinestra3,4, Gilles Favre3,4, S&eacute;bastien Pinaud1, Child&eacute;rick S&eacute;verac1,2, Jean-Charles Faye3,4, Christophe Vieu1,21LAAS-CNRS; Universit&eacute; de Toulouse, Toulouse, France; 2Universit&eacute; de Toulouse, UPS, INSA, INP, ISAE; LAAS; Toulouse, France; 3INSERM U563, Universit&eacute; de Toulouse, CPTP, &ldquo;Signalisation Cellulaire, GTPase Rho et cancers&rdquo;, Toulouse, France; 4Institut Claudius Regaud, Biology Department, Toulouse, FranceAbstract: We present a technique for the label-free detection and recognition of cancer biomarkers using metal nanoislands intended to be integrated in a novel type of nanobiosensor. His-tagged (scFv)-F7N1N2 is the antibody fragment which is directly immobilized, by coordinative bonds, onto ~5 nm nickel islands, then deposited on the surface of a quartz crystal of a quartz crystal microbalance (QCM) to validate the technique. Biomarker GTPase RhoA was investigated because it has been found to be overexpressed in various tumors and because we have recently isolated and characterized a new conformational scFv which selectively recognizes the active form of RhoA. We implemented a surface chemistry involving an antibiofouling coating of polyethylene glycol silane (PEG-silane) (&lt;2 nm thick) and Ni nanoislands to reach a label-free detection of the active antigen conformation of RhoA, at various concentrations. The methodology proposed here proves the viability of the concept by using Ni nanoislands as an anchoring surface layer enabling the detection of a specific conformation of a protein, identified as a potential cancer biomarker. Hence, this novel methodology can be transferred to a nanobiosensor to detect, at lower time consumption and with high sensitivity, specific biomolecules.Keywords: nickel nanoislands, cancer biomarkers, quartz crystal microbalance, PEG-silane, RhoA protein, nanobiosensor
format	article
author	Adri&aacute;n Mart&iacute;nez-Rivas Patrick Chinestra Gilles Favre et al
author_facet	Adri&aacute;n Mart&iacute;nez-Rivas Patrick Chinestra Gilles Favre et al
author_sort	Adri&aacute;n Mart&iacute;nez-Rivas
title	Detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance
title_short	Detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance
title_full	Detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance
title_fullStr	Detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance
title_full_unstemmed	Detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance
title_sort	detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/fd46431a857a47b9a95fac2b4e3b9b09
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Detection of label-free cancer biomarkers using nickel nanoislands and quartz crystal microbalance

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