Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.

Developing methods that evaluate the cellular uptake of magnetic nanoparticles (MNPs) and nanotoxicity effects at single-cellular level are needed. In this study, magnetophoresis combining fluorescence based cytotoxicity assay was proposed to assess the viability and the single-cellular MNPs uptake...

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Autores principales:	Chen-Yu Huang, Tzong-Rong Ger, Zung-Hang Wei, Mei-Feng Lai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd4774548bf7453b8b78b2f26eb12ae2
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spelling	oai:doaj.org-article:fd4774548bf7453b8b78b2f26eb12ae22021-11-18T08:19:33ZCompare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.1932-620310.1371/journal.pone.0096550https://doaj.org/article/fd4774548bf7453b8b78b2f26eb12ae22014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24824523/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Developing methods that evaluate the cellular uptake of magnetic nanoparticles (MNPs) and nanotoxicity effects at single-cellular level are needed. In this study, magnetophoresis combining fluorescence based cytotoxicity assay was proposed to assess the viability and the single-cellular MNPs uptake simultaneously. Malignant cells (SKHep-1, HepG2, HeLa) were incubated with 10 nm anionic iron oxide nanoparticles. Prussian blue stain was performed to visualize the distribution of magnetic nanoparticles. MTT and fluorescence based assay analyzed the cytotoxicity effects of the bulk cell population and single cell, respectively. DAPI/PI stained was applied to evaluate death mechanism. The number of intracellular MNPs was found to be strongly correlated with the cell death. Significant differences between cellular MNP uptake in living and dead cells were observed. The method could be useful for future study of the nanotoxicity induced by MNPs.Chen-Yu HuangTzong-Rong GerZung-Hang WeiMei-Feng LaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96550 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen-Yu Huang Tzong-Rong Ger Zung-Hang Wei Mei-Feng Lai Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.
description	Developing methods that evaluate the cellular uptake of magnetic nanoparticles (MNPs) and nanotoxicity effects at single-cellular level are needed. In this study, magnetophoresis combining fluorescence based cytotoxicity assay was proposed to assess the viability and the single-cellular MNPs uptake simultaneously. Malignant cells (SKHep-1, HepG2, HeLa) were incubated with 10 nm anionic iron oxide nanoparticles. Prussian blue stain was performed to visualize the distribution of magnetic nanoparticles. MTT and fluorescence based assay analyzed the cytotoxicity effects of the bulk cell population and single cell, respectively. DAPI/PI stained was applied to evaluate death mechanism. The number of intracellular MNPs was found to be strongly correlated with the cell death. Significant differences between cellular MNP uptake in living and dead cells were observed. The method could be useful for future study of the nanotoxicity induced by MNPs.
format	article
author	Chen-Yu Huang Tzong-Rong Ger Zung-Hang Wei Mei-Feng Lai
author_facet	Chen-Yu Huang Tzong-Rong Ger Zung-Hang Wei Mei-Feng Lai
author_sort	Chen-Yu Huang
title	Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.
title_short	Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.
title_full	Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.
title_fullStr	Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.
title_full_unstemmed	Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.
title_sort	compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fd4774548bf7453b8b78b2f26eb12ae2
work_keys_str_mv	AT chenyuhuang compareanalysisforthenanotoxicityeffectsofdifferentamountsofendocyticironoxidenanoparticlesatsinglecelllevel AT tzongrongger compareanalysisforthenanotoxicityeffectsofdifferentamountsofendocyticironoxidenanoparticlesatsinglecelllevel AT zunghangwei compareanalysisforthenanotoxicityeffectsofdifferentamountsofendocyticironoxidenanoparticlesatsinglecelllevel AT meifenglai compareanalysisforthenanotoxicityeffectsofdifferentamountsofendocyticironoxidenanoparticlesatsinglecelllevel
_version_	1718421961712861184

Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level.

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