Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

Abstract Salty taste is one of the five basic tastes and is often elicited by NaCl. Because excess sodium intake is associated with many health problems, it could be useful to have salt taste enhancers that are not sodium based. In this study, the regulation of NaCl-induced responses was investigate...

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Autores principales:	Jiao-Jiao Xu, Nadia Elkaddi, Alvaro Garcia-Blanco, Andrew I. Spielman, Alexander A. Bachmanov, Hau Yin Chung, Mehmet Hakan Ozdener
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd4823b3335a414383016f8a69f0c7ba
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spelling	oai:doaj.org-article:fd4823b3335a414383016f8a69f0c7ba2021-12-02T15:06:05ZArginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells10.1038/s41598-017-07756-x2045-2322https://doaj.org/article/fd4823b3335a414383016f8a69f0c7ba2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07756-xhttps://doaj.org/toc/2045-2322Abstract Salty taste is one of the five basic tastes and is often elicited by NaCl. Because excess sodium intake is associated with many health problems, it could be useful to have salt taste enhancers that are not sodium based. In this study, the regulation of NaCl-induced responses was investigated in cultured human fungiform taste papillae (HBO) cells with five arginyl dipeptides: Ala-Arg (AR), Arg-Ala (RA), Arg-Pro (RP), Arg-Glu (RE), and Glu-Arg (ER); and two non-arginyl dipeptides: Asp-Asp (DD) and Glu-Asp (ED). AR, RA, and RP significantly increased the number of cell responses to NaCl, whereas no effect was observed with RE, ER, DD, or ED. We also found no effects with alanine, arginine, or a mixture of both amino acids. Pharmacological studies showed that AR significantly increased responses of amiloride-sensitive but not amiloride-insensitive cells. In studies using small interfering RNAs (siRNAs), responses to AR were significantly decreased in cells transfected with siRNAs against epithelial sodium channel ENaCα or ENaCδ compared to untransfected cells. AR dramatically increased NaCl-elicited responses in cells transfected with NHE1 siRNA but not in those transfected with ENaCα or ENaCδ siRNAs. Altogether, AR increased responses of amiloride-sensitive cells required ENaCα and ENaCδ.Jiao-Jiao XuNadia ElkaddiAlvaro Garcia-BlancoAndrew I. SpielmanAlexander A. BachmanovHau Yin ChungMehmet Hakan OzdenerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiao-Jiao Xu Nadia Elkaddi Alvaro Garcia-Blanco Andrew I. Spielman Alexander A. Bachmanov Hau Yin Chung Mehmet Hakan Ozdener Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells
description	Abstract Salty taste is one of the five basic tastes and is often elicited by NaCl. Because excess sodium intake is associated with many health problems, it could be useful to have salt taste enhancers that are not sodium based. In this study, the regulation of NaCl-induced responses was investigated in cultured human fungiform taste papillae (HBO) cells with five arginyl dipeptides: Ala-Arg (AR), Arg-Ala (RA), Arg-Pro (RP), Arg-Glu (RE), and Glu-Arg (ER); and two non-arginyl dipeptides: Asp-Asp (DD) and Glu-Asp (ED). AR, RA, and RP significantly increased the number of cell responses to NaCl, whereas no effect was observed with RE, ER, DD, or ED. We also found no effects with alanine, arginine, or a mixture of both amino acids. Pharmacological studies showed that AR significantly increased responses of amiloride-sensitive but not amiloride-insensitive cells. In studies using small interfering RNAs (siRNAs), responses to AR were significantly decreased in cells transfected with siRNAs against epithelial sodium channel ENaCα or ENaCδ compared to untransfected cells. AR dramatically increased NaCl-elicited responses in cells transfected with NHE1 siRNA but not in those transfected with ENaCα or ENaCδ siRNAs. Altogether, AR increased responses of amiloride-sensitive cells required ENaCα and ENaCδ.
format	article
author	Jiao-Jiao Xu Nadia Elkaddi Alvaro Garcia-Blanco Andrew I. Spielman Alexander A. Bachmanov Hau Yin Chung Mehmet Hakan Ozdener
author_facet	Jiao-Jiao Xu Nadia Elkaddi Alvaro Garcia-Blanco Andrew I. Spielman Alexander A. Bachmanov Hau Yin Chung Mehmet Hakan Ozdener
author_sort	Jiao-Jiao Xu
title	Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells
title_short	Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells
title_full	Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells
title_fullStr	Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells
title_full_unstemmed	Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells
title_sort	arginyl dipeptides increase the frequency of nacl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fd4823b3335a414383016f8a69f0c7ba
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Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells

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