A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran

A simple and rapid protocol for somatic embryogenesis in banana cv. Nendran (AAB) using immature male flowers (IMF) has been developed. The IMF produced palewhite to yellow, globular embryogenic callus on MS medium supplemented with BA (0.05 - 0.50mgL-1) and picloram (0.50 - 2.00mgL-1) with explant...

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Autores principales:	R S Lekshmi, K B Soni, Swapna Alex, Deepa S Nair, Lekha Sreekantan, B R Reghunath
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2016
Materias:	immature male flowers somatic embryogenesis picloram musa spp. nendran Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/fd4d8c7504414baa87ca5f4132e3686d
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spelling	oai:doaj.org-article:fd4d8c7504414baa87ca5f4132e3686d2021-12-02T15:17:42ZA Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran0973-354X2582-4899https://doaj.org/article/fd4d8c7504414baa87ca5f4132e3686d2016-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/81https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899A simple and rapid protocol for somatic embryogenesis in banana cv. Nendran (AAB) using immature male flowers (IMF) has been developed. The IMF produced palewhite to yellow, globular embryogenic callus on MS medium supplemented with BA (0.05 - 0.50mgL-1) and picloram (0.50 - 2.00mgL-1) with explant response of to 30 per cent. Addition of ascorbic acid (20mgL-1) and Gelrite© (0.45 per cent) to callus induction medium reduced interference from phenolic exudation. Embryogenesis was induced (33.3 to 60 per cent) on semisolid (0.30 per cent Gelrite©) MS medium supplemented with BA 2mgL-1 + IAA 0.5mgL-1. The somatic embryos showed 60-80 per cent germination on half- strength semisolid MS medium with BA 2mgL-1 + IAA 0.5mgL-1. Transfer of germinated embryos to semisolid MS medium supplemented with BA 2mgL-1 + NAA 1mgL-1under 14 h light /8h dark photoperiod resulted in hundred percent conversion to plantlets. This protocol takes merely 6 months for producing plantlets from immature flower buds through somatic embryogenesis, without any intermediate liquid cultures.R S LekshmiK B SoniSwapna AlexDeepa S NairLekha SreekantanB R ReghunathSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleimmature male flowerssomatic embryogenesispiclorammusa spp.nendranPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 11, Iss 2, Pp 116-123 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	immature male flowers somatic embryogenesis picloram musa spp. nendran Plant culture SB1-1110
spellingShingle	immature male flowers somatic embryogenesis picloram musa spp. nendran Plant culture SB1-1110 R S Lekshmi K B Soni Swapna Alex Deepa S Nair Lekha Sreekantan B R Reghunath A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran
description	A simple and rapid protocol for somatic embryogenesis in banana cv. Nendran (AAB) using immature male flowers (IMF) has been developed. The IMF produced palewhite to yellow, globular embryogenic callus on MS medium supplemented with BA (0.05 - 0.50mgL-1) and picloram (0.50 - 2.00mgL-1) with explant response of to 30 per cent. Addition of ascorbic acid (20mgL-1) and Gelrite© (0.45 per cent) to callus induction medium reduced interference from phenolic exudation. Embryogenesis was induced (33.3 to 60 per cent) on semisolid (0.30 per cent Gelrite©) MS medium supplemented with BA 2mgL-1 + IAA 0.5mgL-1. The somatic embryos showed 60-80 per cent germination on half- strength semisolid MS medium with BA 2mgL-1 + IAA 0.5mgL-1. Transfer of germinated embryos to semisolid MS medium supplemented with BA 2mgL-1 + NAA 1mgL-1under 14 h light /8h dark photoperiod resulted in hundred percent conversion to plantlets. This protocol takes merely 6 months for producing plantlets from immature flower buds through somatic embryogenesis, without any intermediate liquid cultures.
format	article
author	R S Lekshmi K B Soni Swapna Alex Deepa S Nair Lekha Sreekantan B R Reghunath
author_facet	R S Lekshmi K B Soni Swapna Alex Deepa S Nair Lekha Sreekantan B R Reghunath
author_sort	R S Lekshmi
title	A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran
title_short	A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran
title_full	A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran
title_fullStr	A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran
title_full_unstemmed	A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran
title_sort	rapid protocol for somatic embryogenesis mediated regeneration in banana (musa spp.) cv. nendran
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2016
url	https://doaj.org/article/fd4d8c7504414baa87ca5f4132e3686d
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A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran

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