Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression

Katie H Sizeland,1 Hannah C Wells,2 Nigel M Kirby,3 Adrian Hawley,3 Stephen T Mudie,3 Tim M Ryan,3 Richard G Haverkamp2 1Human Health, ANSTO, Lucas Heights, Sydney, NSW 2234, Australia; 2School of Food and Advanced Technology, Massey University, Palmerston North 4472, New Zealand; 3SAXS/WAXS Beamlin...

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Autores principales:	Sizeland KH, Wells HC, Kirby NM, Hawley A, Mudie ST, Ryan TM, Haverkamp RG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	collagen meniscus compression saxs scaffold. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fd50b6d0671e4e2ea4a7a622953d7c74
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spelling	oai:doaj.org-article:fd50b6d0671e4e2ea4a7a622953d7c742021-12-02T05:22:56ZBovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression1178-2013https://doaj.org/article/fd50b6d0671e4e2ea4a7a622953d7c742020-07-01T00:00:00Zhttps://www.dovepress.com/bovine-meniscus-middle-zone-tissue-measurement-of-collagen-fibril-beha-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Katie H Sizeland,1 Hannah C Wells,2 Nigel M Kirby,3 Adrian Hawley,3 Stephen T Mudie,3 Tim M Ryan,3 Richard G Haverkamp2 1Human Health, ANSTO, Lucas Heights, Sydney, NSW 2234, Australia; 2School of Food and Advanced Technology, Massey University, Palmerston North 4472, New Zealand; 3SAXS/WAXS Beamline, Australian Synchrotron, ANSTO, Clayton, Melbourne, VIC 3168, AustraliaCorrespondence: Richard G HaverkampSchool of Food and Advanced Technology, Massey University, Private Bag 1112, Palmerston North 4472, New ZealandTel +64 6 356 9099Email r.haverkamp@massey.ac.nzBackground: Type I collagen is the major component of the extracellular matrix of the knee’s meniscus and plays a central role in that joint’s biomechanical properties. Repair and reconstruction of tissue damage often requires a scaffold to assist the body to rebuild. The middle zone of bovine meniscus is a material that may be useful for the preparation of extracellular matrix scaffolds.Methods: Here, synchrotron-based small-angle X-ray scattering (SAXS) patterns of bovine meniscus were collected during unconfined compression. Collagen fibril orientation, D-spacing, compression distance and force were measured.Results: The collagen fibrils in middle zone meniscal fibrocartilage become more highly oriented perpendicular to the direction of compression. The D-spacing also increases, from 65.0 to 66.3 nm with compression up to 0.43 MPa, representing a 1.8% elongation of collagen fibrils perpendicular to the compression.Conclusion: The elasticity of the collagen fibrils under tension along their length when the meniscus is compressed, therefore, contributes to the overall elastic response of the meniscus only under loads that exceed those likely to be experienced physiologically.Keywords: collagen, meniscus, compression, SAXS, scaffoldSizeland KHWells HCKirby NMHawley AMudie STRyan TMHaverkamp RGDove Medical Pressarticlecollagenmeniscuscompressionsaxsscaffold.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 5289-5298 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	collagen meniscus compression saxs scaffold. Medicine (General) R5-920
spellingShingle	collagen meniscus compression saxs scaffold. Medicine (General) R5-920 Sizeland KH Wells HC Kirby NM Hawley A Mudie ST Ryan TM Haverkamp RG Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression
description	Katie H Sizeland,1 Hannah C Wells,2 Nigel M Kirby,3 Adrian Hawley,3 Stephen T Mudie,3 Tim M Ryan,3 Richard G Haverkamp2 1Human Health, ANSTO, Lucas Heights, Sydney, NSW 2234, Australia; 2School of Food and Advanced Technology, Massey University, Palmerston North 4472, New Zealand; 3SAXS/WAXS Beamline, Australian Synchrotron, ANSTO, Clayton, Melbourne, VIC 3168, AustraliaCorrespondence: Richard G HaverkampSchool of Food and Advanced Technology, Massey University, Private Bag 1112, Palmerston North 4472, New ZealandTel +64 6 356 9099Email r.haverkamp@massey.ac.nzBackground: Type I collagen is the major component of the extracellular matrix of the knee’s meniscus and plays a central role in that joint’s biomechanical properties. Repair and reconstruction of tissue damage often requires a scaffold to assist the body to rebuild. The middle zone of bovine meniscus is a material that may be useful for the preparation of extracellular matrix scaffolds.Methods: Here, synchrotron-based small-angle X-ray scattering (SAXS) patterns of bovine meniscus were collected during unconfined compression. Collagen fibril orientation, D-spacing, compression distance and force were measured.Results: The collagen fibrils in middle zone meniscal fibrocartilage become more highly oriented perpendicular to the direction of compression. The D-spacing also increases, from 65.0 to 66.3 nm with compression up to 0.43 MPa, representing a 1.8% elongation of collagen fibrils perpendicular to the compression.Conclusion: The elasticity of the collagen fibrils under tension along their length when the meniscus is compressed, therefore, contributes to the overall elastic response of the meniscus only under loads that exceed those likely to be experienced physiologically.Keywords: collagen, meniscus, compression, SAXS, scaffold
format	article
author	Sizeland KH Wells HC Kirby NM Hawley A Mudie ST Ryan TM Haverkamp RG
author_facet	Sizeland KH Wells HC Kirby NM Hawley A Mudie ST Ryan TM Haverkamp RG
author_sort	Sizeland KH
title	Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression
title_short	Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression
title_full	Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression
title_fullStr	Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression
title_full_unstemmed	Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression
title_sort	bovine meniscus middle zone tissue: measurement of collagen fibril behavior during compression
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/fd50b6d0671e4e2ea4a7a622953d7c74
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Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression

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