A modified approach to induce predictable congestive heart failure by volume overload in rats.

The model of infrarenal aortocaval fistula (ACF) has recently gained new interest in its use to investigate cardiac pathophysiology. Since in previous investigations the development of congestive heart failure (CHF) was inconsistent and started to develop earliest 8-10 weeks after fistula induction...

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Autores principales:	Sascha Treskatsch, Aarne Feldheiser, Adrian T Rosin, Marco Sifringer, Helmut Habazettl, Shaaban A Mousa, Mehdi Shakibaei, Michael Schäfer, Claudia D Spies
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd62cb6bade446868c6321db0004d4e0
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spelling	oai:doaj.org-article:fd62cb6bade446868c6321db0004d4e02021-11-18T08:34:31ZA modified approach to induce predictable congestive heart failure by volume overload in rats.1932-620310.1371/journal.pone.0087531https://doaj.org/article/fd62cb6bade446868c6321db0004d4e02014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498127/?tool=EBIhttps://doaj.org/toc/1932-6203The model of infrarenal aortocaval fistula (ACF) has recently gained new interest in its use to investigate cardiac pathophysiology. Since in previous investigations the development of congestive heart failure (CHF) was inconsistent and started to develop earliest 8-10 weeks after fistula induction using a 18G needle, this project aimed to induce a predictable degree of CHF within a definite time period using a modified approach. An aortocaval fistula was induced in male Wistar rats using a 16G needle as a modification of the former 18G needle-technique described by Garcia and Diebold. Results revealed within 28 ± 2 days of ACF significantly increased heart and lung weight indices in the ACF group accompanied by elevated filling pressure. All hemodynamic parameters derived from a pressure-volume conductance-catheter in vivo were significantly altered in the ACF consistent with severe systolic and diastolic left ventricular dysfunction. This was accompanied by systemic neurohumoral activation as demonstrated by elevated rBNP-45 plasma concentrations in every rat of the ACF group. Furthermore, the restriction in overall cardiac function was associated with a β1- and β2-adrenoreceptor mRNA downregulation in the left ventricle. In contrast, β3-adrenoreceptor mRNA was upregulated. Finally, electron microscopy of the left ventricle of rats in the ACF group showed signs of progressive subcellular myocardial fragmentation. In conclusion, the morphometric, hemodynamic and neurohumoral characterization of the modified approach revealed predictable and consistent signs of congestive heart failure within 28 ± 2 days. Therefore, this modified approach might facilitate the examination of various questions specific to CHF and allow for pharmacological interventions to determine pathophysiological pathways.Sascha TreskatschAarne FeldheiserAdrian T RosinMarco SifringerHelmut HabazettlShaaban A MousaMehdi ShakibaeiMichael SchäferClaudia D SpiesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87531 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sascha Treskatsch Aarne Feldheiser Adrian T Rosin Marco Sifringer Helmut Habazettl Shaaban A Mousa Mehdi Shakibaei Michael Schäfer Claudia D Spies A modified approach to induce predictable congestive heart failure by volume overload in rats.
description	The model of infrarenal aortocaval fistula (ACF) has recently gained new interest in its use to investigate cardiac pathophysiology. Since in previous investigations the development of congestive heart failure (CHF) was inconsistent and started to develop earliest 8-10 weeks after fistula induction using a 18G needle, this project aimed to induce a predictable degree of CHF within a definite time period using a modified approach. An aortocaval fistula was induced in male Wistar rats using a 16G needle as a modification of the former 18G needle-technique described by Garcia and Diebold. Results revealed within 28 ± 2 days of ACF significantly increased heart and lung weight indices in the ACF group accompanied by elevated filling pressure. All hemodynamic parameters derived from a pressure-volume conductance-catheter in vivo were significantly altered in the ACF consistent with severe systolic and diastolic left ventricular dysfunction. This was accompanied by systemic neurohumoral activation as demonstrated by elevated rBNP-45 plasma concentrations in every rat of the ACF group. Furthermore, the restriction in overall cardiac function was associated with a β1- and β2-adrenoreceptor mRNA downregulation in the left ventricle. In contrast, β3-adrenoreceptor mRNA was upregulated. Finally, electron microscopy of the left ventricle of rats in the ACF group showed signs of progressive subcellular myocardial fragmentation. In conclusion, the morphometric, hemodynamic and neurohumoral characterization of the modified approach revealed predictable and consistent signs of congestive heart failure within 28 ± 2 days. Therefore, this modified approach might facilitate the examination of various questions specific to CHF and allow for pharmacological interventions to determine pathophysiological pathways.
format	article
author	Sascha Treskatsch Aarne Feldheiser Adrian T Rosin Marco Sifringer Helmut Habazettl Shaaban A Mousa Mehdi Shakibaei Michael Schäfer Claudia D Spies
author_facet	Sascha Treskatsch Aarne Feldheiser Adrian T Rosin Marco Sifringer Helmut Habazettl Shaaban A Mousa Mehdi Shakibaei Michael Schäfer Claudia D Spies
author_sort	Sascha Treskatsch
title	A modified approach to induce predictable congestive heart failure by volume overload in rats.
title_short	A modified approach to induce predictable congestive heart failure by volume overload in rats.
title_full	A modified approach to induce predictable congestive heart failure by volume overload in rats.
title_fullStr	A modified approach to induce predictable congestive heart failure by volume overload in rats.
title_full_unstemmed	A modified approach to induce predictable congestive heart failure by volume overload in rats.
title_sort	modified approach to induce predictable congestive heart failure by volume overload in rats.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fd62cb6bade446868c6321db0004d4e0
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A modified approach to induce predictable congestive heart failure by volume overload in rats.

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