DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.

Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal conservation-especially for endangered species such as members...

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Autores principales:	Chodon Sass, Damon P Little, Dennis Wm Stevenson, Chelsea D Specht
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd63f27fb6db49d18d8686802719d344
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spelling	oai:doaj.org-article:fd63f27fb6db49d18d8686802719d3442021-11-25T06:10:31ZDNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.1932-620310.1371/journal.pone.0001154https://doaj.org/article/fd63f27fb6db49d18d8686802719d3442007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001154https://doaj.org/toc/1932-6203Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal conservation-especially for endangered species such as members of the Cycadales. Ideally, barcodes could be used to positively identify illegally obtained material even in cases where diagnostic features have been purposefully removed or to release confiscated organisms into the proper breeding population. In order to be useful, a DNA barcode sequence must not only easily PCR amplify with universal or near-universal reaction conditions and primers, but also contain enough variation to generate unique identifiers at either the species or population levels. Chloroplast regions suggested by the Plant Working Group of the Consortium for the Barcode of Life (CBoL), and two alternatives, the chloroplast psbA-trnH intergenic spacer and the nuclear ribosomal internal transcribed spacer (nrITS), were tested for their utility in generating unique identifiers for members of the Cycadales. Ease of amplification and sequence generation with universal primers and reaction conditions was determined for each of the seven proposed markers. While none of the proposed markers provided unique identifiers for all species tested, nrITS showed the most promise in terms of variability, although sequencing difficulties remain a drawback. We suggest a workflow for DNA barcoding, including database generation and management, which will ultimately be necessary if we are to succeed in establishing a universal DNA barcode for plants.Chodon SassDamon P LittleDennis Wm StevensonChelsea D SpechtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 11, p e1154 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chodon Sass Damon P Little Dennis Wm Stevenson Chelsea D Specht DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.
description	Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal conservation-especially for endangered species such as members of the Cycadales. Ideally, barcodes could be used to positively identify illegally obtained material even in cases where diagnostic features have been purposefully removed or to release confiscated organisms into the proper breeding population. In order to be useful, a DNA barcode sequence must not only easily PCR amplify with universal or near-universal reaction conditions and primers, but also contain enough variation to generate unique identifiers at either the species or population levels. Chloroplast regions suggested by the Plant Working Group of the Consortium for the Barcode of Life (CBoL), and two alternatives, the chloroplast psbA-trnH intergenic spacer and the nuclear ribosomal internal transcribed spacer (nrITS), were tested for their utility in generating unique identifiers for members of the Cycadales. Ease of amplification and sequence generation with universal primers and reaction conditions was determined for each of the seven proposed markers. While none of the proposed markers provided unique identifiers for all species tested, nrITS showed the most promise in terms of variability, although sequencing difficulties remain a drawback. We suggest a workflow for DNA barcoding, including database generation and management, which will ultimately be necessary if we are to succeed in establishing a universal DNA barcode for plants.
format	article
author	Chodon Sass Damon P Little Dennis Wm Stevenson Chelsea D Specht
author_facet	Chodon Sass Damon P Little Dennis Wm Stevenson Chelsea D Specht
author_sort	Chodon Sass
title	DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.
title_short	DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.
title_full	DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.
title_fullStr	DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.
title_full_unstemmed	DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.
title_sort	dna barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/fd63f27fb6db49d18d8686802719d344
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DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads.

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