Exercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex

Ageing is a major risk factor for many of the most prevalent diseases, including neurodegenerative diseases, cancer, and heart disease. As the global population continues to age, behavioural interventions that can promote healthy ageing will improve quality of life and relieve the socioeconomic burd...

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Autores principales:	Brad Ebanks, Ying Wang, Gunjan Katyal, Chloe Sargent, Thomas L. Ingram, Antonia Bowman, Nicoleta Moisoi, Lisa Chakrabarti
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mitochondria ageing exercise <i>Drosophila</i> respirometry lifespan Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fd6e202bdb9f40d9aab864250b4de774
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spelling	oai:doaj.org-article:fd6e202bdb9f40d9aab864250b4de7742021-11-11T17:05:24ZExercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex10.3390/ijms2221116061422-00671661-6596https://doaj.org/article/fd6e202bdb9f40d9aab864250b4de7742021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11606https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Ageing is a major risk factor for many of the most prevalent diseases, including neurodegenerative diseases, cancer, and heart disease. As the global population continues to age, behavioural interventions that can promote healthy ageing will improve quality of life and relieve the socioeconomic burden that comes with an aged society. Exercise is recognised as an effective intervention against many diseases of ageing, but we do not know the stage in an individual’s lifetime at which exercise is most effective at promoting healthy ageing, and whether or not it has a direct effect on lifespan. We exercised <i>w<sup>1118</sup> Drosophila melanogaster</i>, investigating the effects of sex and group size at different stages of their lifetime, and recorded their lifespan. Climbing scores at 30 days were measured to record differences in fitness in response to exercise. We also assessed the mitochondrial proteome of <i>w<sup>1118</sup> Drosophila</i> that had been exercised for one week, alongside mitochondrial respiration measured using high-resolution respirometry, to determine changes in mitochondrial physiology in response to exercise. We found that age-targeted exercise interventions improved the lifespan of both male and female <i>Drosophila</i>, and grouped males exercised in late life had improved climbing scores when compared with those exercised throughout their entire lifespan. The proteins of the electron transport chain were significantly upregulated in expression after one week of exercise, and complex-II-linked respiration was significantly increased in exercised <i>Drosophila</i>. Taken together, our findings provide a basis to test specific proteins, and complex II of the respiratory chain, as important effectors of exercise-induced healthy ageing.Brad EbanksYing WangGunjan KatyalChloe SargentThomas L. IngramAntonia BowmanNicoleta MoisoiLisa ChakrabartiMDPI AGarticlemitochondriaageingexercise<i>Drosophila</i>respirometrylifespanBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11606, p 11606 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mitochondria ageing exercise <i>Drosophila</i> respirometry lifespan Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	mitochondria ageing exercise <i>Drosophila</i> respirometry lifespan Biology (General) QH301-705.5 Chemistry QD1-999 Brad Ebanks Ying Wang Gunjan Katyal Chloe Sargent Thomas L. Ingram Antonia Bowman Nicoleta Moisoi Lisa Chakrabarti Exercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex
description	Ageing is a major risk factor for many of the most prevalent diseases, including neurodegenerative diseases, cancer, and heart disease. As the global population continues to age, behavioural interventions that can promote healthy ageing will improve quality of life and relieve the socioeconomic burden that comes with an aged society. Exercise is recognised as an effective intervention against many diseases of ageing, but we do not know the stage in an individual’s lifetime at which exercise is most effective at promoting healthy ageing, and whether or not it has a direct effect on lifespan. We exercised <i>w<sup>1118</sup> Drosophila melanogaster</i>, investigating the effects of sex and group size at different stages of their lifetime, and recorded their lifespan. Climbing scores at 30 days were measured to record differences in fitness in response to exercise. We also assessed the mitochondrial proteome of <i>w<sup>1118</sup> Drosophila</i> that had been exercised for one week, alongside mitochondrial respiration measured using high-resolution respirometry, to determine changes in mitochondrial physiology in response to exercise. We found that age-targeted exercise interventions improved the lifespan of both male and female <i>Drosophila</i>, and grouped males exercised in late life had improved climbing scores when compared with those exercised throughout their entire lifespan. The proteins of the electron transport chain were significantly upregulated in expression after one week of exercise, and complex-II-linked respiration was significantly increased in exercised <i>Drosophila</i>. Taken together, our findings provide a basis to test specific proteins, and complex II of the respiratory chain, as important effectors of exercise-induced healthy ageing.
format	article
author	Brad Ebanks Ying Wang Gunjan Katyal Chloe Sargent Thomas L. Ingram Antonia Bowman Nicoleta Moisoi Lisa Chakrabarti
author_facet	Brad Ebanks Ying Wang Gunjan Katyal Chloe Sargent Thomas L. Ingram Antonia Bowman Nicoleta Moisoi Lisa Chakrabarti
author_sort	Brad Ebanks
title	Exercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex
title_short	Exercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex
title_full	Exercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex
title_fullStr	Exercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex
title_full_unstemmed	Exercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex
title_sort	exercising <i>d. melanogaster</i> modulates the mitochondrial proteome and physiology. the effect on lifespan depends upon age and sex
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fd6e202bdb9f40d9aab864250b4de774
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Exercising <i>D. melanogaster</i> Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex

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