Exosomal lncRNA HOTTIP Mediates Antiviral Effect of Tenofovir Alafenamide (TAF) on HBV Infection
Qing-Min Liu,1,* Yi-Yu He,2,* Li-Li Liu,3 Li-Kun Wang4 1Intensive Care Unit, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of China; 2Department of Cardiovascular Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, People’s Republic of China; 3...
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2021
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oai:doaj.org-article:fd79de68b1cf45d1824f91eb6955989e2021-12-02T19:39:19ZExosomal lncRNA HOTTIP Mediates Antiviral Effect of Tenofovir Alafenamide (TAF) on HBV Infection1178-7031https://doaj.org/article/fd79de68b1cf45d1824f91eb6955989e2021-10-01T00:00:00Zhttps://www.dovepress.com/exosomal-lncrna-hottip-mediates-antiviral-effect-of-tenofovir-alafenam-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Qing-Min Liu,1,* Yi-Yu He,2,* Li-Li Liu,3 Li-Kun Wang4 1Intensive Care Unit, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of China; 2Department of Cardiovascular Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, People’s Republic of China; 3Department of Pathology, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of China; 4Infection Control Center, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Li-Li LiuDepartment of Pathology, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of ChinaEmail 13505495928@126.comLi-Kun WangInfection Control Center, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of ChinaEmail Lkwang999@163.comIntroduction: Chronic hepatitis B (CHB) virus (HBV) infection has emerged as a global health burden affecting nearly 292 million people. Tenofovir alafenamide (TAF) is an effective treatment for CHB patients. However, the detailed mechanism underlying the antiviral activity of TAF remains unclear.Methods: In this study, we investigated the antiviral effect of exosomes derived from the serum of CHB patients treated with TAF (Exo-serum) and TAF-treated macrophages (MP) (Exo-MP(TAF)).Results: RNAseq analysis was also performed to determine the associated long non-coding RNAs (lncRNAs). The results demonstrated that both Exo-serum and Exo-MP(TAF) could be taken up by HepAD38 cells and exhibited potent antiviral activities, as manifested by significantly downregulating the levels of hepatitis B surface antigen, hepatitis B e antigen, HBV DNA, and covalently closed circular DNA. The antiviral effect of Exo-serum was more potent than those of TAF treatment alone. RNAseq analysis revealed that lncRNA HOTTIP was upregulated significantly in Exo-serum. Further, lncRNA HOTTIP knockdown reversed the antiviral effect of Exo-MP(TAF) on HepAD38 cells, whereas lncRNA HOTTIP knockdown exerted the opposite roles.Discussion: Taken together, these results suggest that exosomal lncRNA HOTTIP is essential for the antiviral activity of TAF and provide a novel understanding of the exosome-mediated mechanism underlying HBV infection.Keywords: chronic hepatitis B, tenofovir alafenamide, exosome, lncRNA HOTTIPLiu QMHe YYLiu LLWang LKDove Medical Pressarticlechronic hepatitis btenofovir alafenamideexosomelncrna hottipPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 5489-5500 (2021) |
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chronic hepatitis b tenofovir alafenamide exosome lncrna hottip Pathology RB1-214 Therapeutics. Pharmacology RM1-950 |
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chronic hepatitis b tenofovir alafenamide exosome lncrna hottip Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Liu QM He YY Liu LL Wang LK Exosomal lncRNA HOTTIP Mediates Antiviral Effect of Tenofovir Alafenamide (TAF) on HBV Infection |
| description |
Qing-Min Liu,1,* Yi-Yu He,2,* Li-Li Liu,3 Li-Kun Wang4 1Intensive Care Unit, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of China; 2Department of Cardiovascular Disease, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, People’s Republic of China; 3Department of Pathology, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of China; 4Infection Control Center, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Li-Li LiuDepartment of Pathology, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of ChinaEmail 13505495928@126.comLi-Kun WangInfection Control Center, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of ChinaEmail Lkwang999@163.comIntroduction: Chronic hepatitis B (CHB) virus (HBV) infection has emerged as a global health burden affecting nearly 292 million people. Tenofovir alafenamide (TAF) is an effective treatment for CHB patients. However, the detailed mechanism underlying the antiviral activity of TAF remains unclear.Methods: In this study, we investigated the antiviral effect of exosomes derived from the serum of CHB patients treated with TAF (Exo-serum) and TAF-treated macrophages (MP) (Exo-MP(TAF)).Results: RNAseq analysis was also performed to determine the associated long non-coding RNAs (lncRNAs). The results demonstrated that both Exo-serum and Exo-MP(TAF) could be taken up by HepAD38 cells and exhibited potent antiviral activities, as manifested by significantly downregulating the levels of hepatitis B surface antigen, hepatitis B e antigen, HBV DNA, and covalently closed circular DNA. The antiviral effect of Exo-serum was more potent than those of TAF treatment alone. RNAseq analysis revealed that lncRNA HOTTIP was upregulated significantly in Exo-serum. Further, lncRNA HOTTIP knockdown reversed the antiviral effect of Exo-MP(TAF) on HepAD38 cells, whereas lncRNA HOTTIP knockdown exerted the opposite roles.Discussion: Taken together, these results suggest that exosomal lncRNA HOTTIP is essential for the antiviral activity of TAF and provide a novel understanding of the exosome-mediated mechanism underlying HBV infection.Keywords: chronic hepatitis B, tenofovir alafenamide, exosome, lncRNA HOTTIP |
| format |
article |
| author |
Liu QM He YY Liu LL Wang LK |
| author_facet |
Liu QM He YY Liu LL Wang LK |
| author_sort |
Liu QM |
| title |
Exosomal lncRNA HOTTIP Mediates Antiviral Effect of Tenofovir Alafenamide (TAF) on HBV Infection |
| title_short |
Exosomal lncRNA HOTTIP Mediates Antiviral Effect of Tenofovir Alafenamide (TAF) on HBV Infection |
| title_full |
Exosomal lncRNA HOTTIP Mediates Antiviral Effect of Tenofovir Alafenamide (TAF) on HBV Infection |
| title_fullStr |
Exosomal lncRNA HOTTIP Mediates Antiviral Effect of Tenofovir Alafenamide (TAF) on HBV Infection |
| title_full_unstemmed |
Exosomal lncRNA HOTTIP Mediates Antiviral Effect of Tenofovir Alafenamide (TAF) on HBV Infection |
| title_sort |
exosomal lncrna hottip mediates antiviral effect of tenofovir alafenamide (taf) on hbv infection |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/fd79de68b1cf45d1824f91eb6955989e |
| work_keys_str_mv |
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| _version_ |
1718376300734840832 |