Monitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method

There is intensive research using gold nanoparticles for biomedical purposes, which have many advantages such as ease of synthesis and high reactivity. Their possible small size (<10 nm) can lead to the crossing of biological membranes and then to problematic dissemination and storage in organs t...

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Autores principales:	Thomas Chaigneau, Arnaud Pallotta, Fatima Zahra Benaddi, Lucie Sancey, Said Chakir, Ariane Boudier, Igor Clarot
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanoparticles gold quantification HPLC validation in vivo Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fd7be9e9a97140199c2bdae7a618c41a
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spelling	oai:doaj.org-article:fd7be9e9a97140199c2bdae7a618c41a2021-11-25T18:59:25ZMonitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method10.3390/separations81102152297-8739https://doaj.org/article/fd7be9e9a97140199c2bdae7a618c41a2021-11-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/215https://doaj.org/toc/2297-8739There is intensive research using gold nanoparticles for biomedical purposes, which have many advantages such as ease of synthesis and high reactivity. Their possible small size (<10 nm) can lead to the crossing of biological membranes and then to problematic dissemination and storage in organs that must be controlled and evaluated. In this work, a simple isocratic HPLC method was developed and validated to quantify the gold coming from nanoparticles in different biological samples. After a first carbonization step at 900 °C, the nanoparticles were oxidized by dibroma under acidic conditions, leading to tetrachloroaurate ions that could form ion pairs when adding rhodamine B. Finally, ion pairs were extracted and rhodamine B was evaluated to quantify the corresponding gold concentration by reversed-phase HPLC with visible detection. The method was validated for different organs (liver, spleen, lungs, kidneys, or brain) and fluids (plasma and urine) from rats and mice. Lastly, the developed method was used to evaluate the content of gold in organs and fluids after intravenous (IV) injection of nanoparticles.Thomas ChaigneauArnaud PallottaFatima Zahra BenaddiLucie SanceySaid ChakirAriane BoudierIgor ClarotMDPI AGarticlenanoparticlesgold quantificationHPLCvalidationin vivoPhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 215, p 215 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticles gold quantification HPLC validation in vivo Physics QC1-999 Chemistry QD1-999
spellingShingle	nanoparticles gold quantification HPLC validation in vivo Physics QC1-999 Chemistry QD1-999 Thomas Chaigneau Arnaud Pallotta Fatima Zahra Benaddi Lucie Sancey Said Chakir Ariane Boudier Igor Clarot Monitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method
description	There is intensive research using gold nanoparticles for biomedical purposes, which have many advantages such as ease of synthesis and high reactivity. Their possible small size (<10 nm) can lead to the crossing of biological membranes and then to problematic dissemination and storage in organs that must be controlled and evaluated. In this work, a simple isocratic HPLC method was developed and validated to quantify the gold coming from nanoparticles in different biological samples. After a first carbonization step at 900 °C, the nanoparticles were oxidized by dibroma under acidic conditions, leading to tetrachloroaurate ions that could form ion pairs when adding rhodamine B. Finally, ion pairs were extracted and rhodamine B was evaluated to quantify the corresponding gold concentration by reversed-phase HPLC with visible detection. The method was validated for different organs (liver, spleen, lungs, kidneys, or brain) and fluids (plasma and urine) from rats and mice. Lastly, the developed method was used to evaluate the content of gold in organs and fluids after intravenous (IV) injection of nanoparticles.
format	article
author	Thomas Chaigneau Arnaud Pallotta Fatima Zahra Benaddi Lucie Sancey Said Chakir Ariane Boudier Igor Clarot
author_facet	Thomas Chaigneau Arnaud Pallotta Fatima Zahra Benaddi Lucie Sancey Said Chakir Ariane Boudier Igor Clarot
author_sort	Thomas Chaigneau
title	Monitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method
title_short	Monitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method
title_full	Monitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method
title_fullStr	Monitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method
title_full_unstemmed	Monitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method
title_sort	monitoring of gold biodistribution from nanoparticles using a hplc-visible method
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fd7be9e9a97140199c2bdae7a618c41a
work_keys_str_mv	AT thomaschaigneau monitoringofgoldbiodistributionfromnanoparticlesusingahplcvisiblemethod AT arnaudpallotta monitoringofgoldbiodistributionfromnanoparticlesusingahplcvisiblemethod AT fatimazahrabenaddi monitoringofgoldbiodistributionfromnanoparticlesusingahplcvisiblemethod AT luciesancey monitoringofgoldbiodistributionfromnanoparticlesusingahplcvisiblemethod AT saidchakir monitoringofgoldbiodistributionfromnanoparticlesusingahplcvisiblemethod AT arianeboudier monitoringofgoldbiodistributionfromnanoparticlesusingahplcvisiblemethod AT igorclarot monitoringofgoldbiodistributionfromnanoparticlesusingahplcvisiblemethod
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Monitoring of Gold Biodistribution from Nanoparticles Using a HPLC-Visible Method

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