Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.

Acute lung injury (ALI) is an important cause of mortality in critically ill patients. Acute pancreatitis (AP) is one of the risk factors for developing this syndrome. Among the inflammatory cells, macrophages have a key role in determining the severity of the acute lung injury. In the lungs, macrop...

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Autores principales:	Hamid Akbarshahi, Mandy Menzel, Monika Posaric Bauden, Ann Rosendahl, Roland Andersson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd808c38bcec4e1dbb2303e9fd5f1599
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spelling	oai:doaj.org-article:fd808c38bcec4e1dbb2303e9fd5f15992021-11-18T08:11:26ZEnrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.1932-620310.1371/journal.pone.0042654https://doaj.org/article/fd808c38bcec4e1dbb2303e9fd5f15992012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110041/?tool=EBIhttps://doaj.org/toc/1932-6203Acute lung injury (ALI) is an important cause of mortality in critically ill patients. Acute pancreatitis (AP) is one of the risk factors for developing this syndrome. Among the inflammatory cells, macrophages have a key role in determining the severity of the acute lung injury. In the lungs, macrophages constitute a heterogeneous cell population distributed in different compartments. Changes in not only the macrophage count, but also in their phenotype have been seen during the course of lung injury. A murine ductal ligation model of acute pancreatitis showed substantial morphological changes in the pancreas and lungs. Immunohistochemistry showed neutrophil recruitment into both organs after 9 hours and later on. F4/80(+) cells in the pancreas increased in the ligated animals, though there was not a significant difference in their number in the lungs as compared to sham operated animals. Flow cytometry analysis of lung macrophages demonstrated an enrichment of F4/80(-) CD68(+)CCR2(+) and F4/80(-) CD68(+)CD206(+) lung macrophages in ligated animals (AP) as compared to the sham operated group. The level of interleukin-6 in plasma increased 3 hours after ligation compared to the sham operated group, as a first indicator of a systemic inflammatory response.This study suggests a role for F4/80(-) CD68(+) macrophages in the pathogenesis of acute lung injury in acute pancreatitis. Studying lung macrophages for different phenotypic markers, their polarization, activation and recruitment, in the context of acute lung injury, is a novel area to potentially identify interventions which may improve the outcome of acute lung injury.Hamid AkbarshahiMandy MenzelMonika Posaric BaudenAnn RosendahlRoland AnderssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e42654 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hamid Akbarshahi Mandy Menzel Monika Posaric Bauden Ann Rosendahl Roland Andersson Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.
description	Acute lung injury (ALI) is an important cause of mortality in critically ill patients. Acute pancreatitis (AP) is one of the risk factors for developing this syndrome. Among the inflammatory cells, macrophages have a key role in determining the severity of the acute lung injury. In the lungs, macrophages constitute a heterogeneous cell population distributed in different compartments. Changes in not only the macrophage count, but also in their phenotype have been seen during the course of lung injury. A murine ductal ligation model of acute pancreatitis showed substantial morphological changes in the pancreas and lungs. Immunohistochemistry showed neutrophil recruitment into both organs after 9 hours and later on. F4/80(+) cells in the pancreas increased in the ligated animals, though there was not a significant difference in their number in the lungs as compared to sham operated animals. Flow cytometry analysis of lung macrophages demonstrated an enrichment of F4/80(-) CD68(+)CCR2(+) and F4/80(-) CD68(+)CD206(+) lung macrophages in ligated animals (AP) as compared to the sham operated group. The level of interleukin-6 in plasma increased 3 hours after ligation compared to the sham operated group, as a first indicator of a systemic inflammatory response.This study suggests a role for F4/80(-) CD68(+) macrophages in the pathogenesis of acute lung injury in acute pancreatitis. Studying lung macrophages for different phenotypic markers, their polarization, activation and recruitment, in the context of acute lung injury, is a novel area to potentially identify interventions which may improve the outcome of acute lung injury.
format	article
author	Hamid Akbarshahi Mandy Menzel Monika Posaric Bauden Ann Rosendahl Roland Andersson
author_facet	Hamid Akbarshahi Mandy Menzel Monika Posaric Bauden Ann Rosendahl Roland Andersson
author_sort	Hamid Akbarshahi
title	Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.
title_short	Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.
title_full	Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.
title_fullStr	Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.
title_full_unstemmed	Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.
title_sort	enrichment of murine cd68+ ccr2+ and cd68+ cd206+ lung macrophages in acute pancreatitis-associated acute lung injury.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/fd808c38bcec4e1dbb2303e9fd5f1599
work_keys_str_mv	AT hamidakbarshahi enrichmentofmurinecd68ccr2andcd68cd206lungmacrophagesinacutepancreatitisassociatedacutelunginjury AT mandymenzel enrichmentofmurinecd68ccr2andcd68cd206lungmacrophagesinacutepancreatitisassociatedacutelunginjury AT monikaposaricbauden enrichmentofmurinecd68ccr2andcd68cd206lungmacrophagesinacutepancreatitisassociatedacutelunginjury AT annrosendahl enrichmentofmurinecd68ccr2andcd68cd206lungmacrophagesinacutepancreatitisassociatedacutelunginjury AT rolandandersson enrichmentofmurinecd68ccr2andcd68cd206lungmacrophagesinacutepancreatitisassociatedacutelunginjury
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Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury.

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