Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance

Abstract DNA is basically an intracellular molecule that stores genetic information and carries instructions for growth and reproduction in all cellular organisms. However, in some bacteria, DNA has additional roles outside the cells as extracellular DNA (eDNA), which is an essential component of bi...

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Autores principales:	Aleksandr Ilinov, Akihito Nishiyama, Hiroki Namba, Yukari Fukushima, Hayato Takihara, Chie Nakajima, Anna Savitskaya, Gebremichal Gebretsadik, Mariko Hakamata, Yuriko Ozeki, Yoshitaka Tateishi, Shujiro Okuda, Yasuhiko Suzuki, Yuri S. Vinnik, Sohkichi Matsumoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd8107f2b9c84f72bfaf95c7ff6b69f6
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spelling	oai:doaj.org-article:fd8107f2b9c84f72bfaf95c7ff6b69f62021-12-02T15:49:50ZExtracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance10.1038/s41598-021-90156-z2045-2322https://doaj.org/article/fd8107f2b9c84f72bfaf95c7ff6b69f62021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90156-zhttps://doaj.org/toc/2045-2322Abstract DNA is basically an intracellular molecule that stores genetic information and carries instructions for growth and reproduction in all cellular organisms. However, in some bacteria, DNA has additional roles outside the cells as extracellular DNA (eDNA), which is an essential component of biofilm formation and hence antibiotic tolerance. Mycobacteria include life-threating human pathogens, most of which are slow growers. However, little is known about the nature of pathogenic mycobacteria’s eDNA. Here we found that eDNA is present in slow-growing mycobacterial pathogens, such as Mycobacterium tuberculosis, M. intracellulare, and M. avium at exponential growth phase. In contrast, eDNA is little in all tested rapid-growing mycobacteria. The physiological impact of disrupted eDNA on slow-growing mycobacteria include reduced pellicle formation, floating biofilm, and enhanced susceptibility to isoniazid and amikacin. Isolation and sequencing of eDNA revealed that it is identical to the genomic DNA in M. tuberculosis and M. intracellulare. In contrast, accumulation of phage DNA in eDNA of M. avium, suggests that the DNA released differs among mycobacterial species. Our data show important functions of eDNA necessary for biofilm formation and drug tolerance in slow-growing mycobacteria.Aleksandr IlinovAkihito NishiyamaHiroki NambaYukari FukushimaHayato TakiharaChie NakajimaAnna SavitskayaGebremichal GebretsadikMariko HakamataYuriko OzekiYoshitaka TateishiShujiro OkudaYasuhiko SuzukiYuri S. VinnikSohkichi MatsumotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleksandr Ilinov Akihito Nishiyama Hiroki Namba Yukari Fukushima Hayato Takihara Chie Nakajima Anna Savitskaya Gebremichal Gebretsadik Mariko Hakamata Yuriko Ozeki Yoshitaka Tateishi Shujiro Okuda Yasuhiko Suzuki Yuri S. Vinnik Sohkichi Matsumoto Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance
description	Abstract DNA is basically an intracellular molecule that stores genetic information and carries instructions for growth and reproduction in all cellular organisms. However, in some bacteria, DNA has additional roles outside the cells as extracellular DNA (eDNA), which is an essential component of biofilm formation and hence antibiotic tolerance. Mycobacteria include life-threating human pathogens, most of which are slow growers. However, little is known about the nature of pathogenic mycobacteria’s eDNA. Here we found that eDNA is present in slow-growing mycobacterial pathogens, such as Mycobacterium tuberculosis, M. intracellulare, and M. avium at exponential growth phase. In contrast, eDNA is little in all tested rapid-growing mycobacteria. The physiological impact of disrupted eDNA on slow-growing mycobacteria include reduced pellicle formation, floating biofilm, and enhanced susceptibility to isoniazid and amikacin. Isolation and sequencing of eDNA revealed that it is identical to the genomic DNA in M. tuberculosis and M. intracellulare. In contrast, accumulation of phage DNA in eDNA of M. avium, suggests that the DNA released differs among mycobacterial species. Our data show important functions of eDNA necessary for biofilm formation and drug tolerance in slow-growing mycobacteria.
format	article
author	Aleksandr Ilinov Akihito Nishiyama Hiroki Namba Yukari Fukushima Hayato Takihara Chie Nakajima Anna Savitskaya Gebremichal Gebretsadik Mariko Hakamata Yuriko Ozeki Yoshitaka Tateishi Shujiro Okuda Yasuhiko Suzuki Yuri S. Vinnik Sohkichi Matsumoto
author_facet	Aleksandr Ilinov Akihito Nishiyama Hiroki Namba Yukari Fukushima Hayato Takihara Chie Nakajima Anna Savitskaya Gebremichal Gebretsadik Mariko Hakamata Yuriko Ozeki Yoshitaka Tateishi Shujiro Okuda Yasuhiko Suzuki Yuri S. Vinnik Sohkichi Matsumoto
author_sort	Aleksandr Ilinov
title	Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance
title_short	Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance
title_full	Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance
title_fullStr	Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance
title_full_unstemmed	Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance
title_sort	extracellular dna of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fd8107f2b9c84f72bfaf95c7ff6b69f6
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Extracellular DNA of slow growers of mycobacteria and its contribution to biofilm formation and drug tolerance

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