Molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions

Abstract Natural accessions of Arabidopsis thaliana differ in their growth and development, but also vary dramatically in their nitrogen use efficiencies (NUE). The molecular basis for these differences has not been addressed yet. Experiments with five contrasting accessions grown in hydroponics at...

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Autores principales:	Jochen Menz, Tim Range, Johannes Trini, Uwe Ludewig, Benjamin Neuhäuser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd866653f79843c18c4ec0ef3c9f83d0
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spelling	oai:doaj.org-article:fd866653f79843c18c4ec0ef3c9f83d02021-12-02T15:08:16ZMolecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions10.1038/s41598-018-21684-42045-2322https://doaj.org/article/fd866653f79843c18c4ec0ef3c9f83d02018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21684-4https://doaj.org/toc/2045-2322Abstract Natural accessions of Arabidopsis thaliana differ in their growth and development, but also vary dramatically in their nitrogen use efficiencies (NUE). The molecular basis for these differences has not been addressed yet. Experiments with five contrasting accessions grown in hydroponics at different levels of inorganic nitrogen confirmed low NUE of Col-0 and higher NUE in Tsu-0. At constant external nitrogen supply, higher NUE was based on nitrogen capture, rather than utilization of nitrogen for shoot biomass. This changed when a limited nitrogen amount was supplied. Nevertheless, the total NUE sequence remained similar. Interestingly, the two most contrasting accessions, Col-0 and Tsu-0, differed in the capture of single inorganic nitrogen sources, reflected by the differential consumption of 15N label from ammonium or nitrate, when supplied together. Tsu-0 acquired more nitrate than Col-0, both in roots and shoots. This preference was directly correlated with the expression of certain nitrogen uptake and assimilation systems in the root. However, early transcriptional responses of the root to nitrate deprivation were similar in both accessions, suggesting that the sensing of the external lack of nitrate was not different in the more nitrogen use efficient accession. Thus, a robust rapid nitrate-deprivation signaling exists in both genotypes.Jochen MenzTim RangeJohannes TriniUwe LudewigBenjamin NeuhäuserNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jochen Menz Tim Range Johannes Trini Uwe Ludewig Benjamin Neuhäuser Molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions
description	Abstract Natural accessions of Arabidopsis thaliana differ in their growth and development, but also vary dramatically in their nitrogen use efficiencies (NUE). The molecular basis for these differences has not been addressed yet. Experiments with five contrasting accessions grown in hydroponics at different levels of inorganic nitrogen confirmed low NUE of Col-0 and higher NUE in Tsu-0. At constant external nitrogen supply, higher NUE was based on nitrogen capture, rather than utilization of nitrogen for shoot biomass. This changed when a limited nitrogen amount was supplied. Nevertheless, the total NUE sequence remained similar. Interestingly, the two most contrasting accessions, Col-0 and Tsu-0, differed in the capture of single inorganic nitrogen sources, reflected by the differential consumption of 15N label from ammonium or nitrate, when supplied together. Tsu-0 acquired more nitrate than Col-0, both in roots and shoots. This preference was directly correlated with the expression of certain nitrogen uptake and assimilation systems in the root. However, early transcriptional responses of the root to nitrate deprivation were similar in both accessions, suggesting that the sensing of the external lack of nitrate was not different in the more nitrogen use efficient accession. Thus, a robust rapid nitrate-deprivation signaling exists in both genotypes.
format	article
author	Jochen Menz Tim Range Johannes Trini Uwe Ludewig Benjamin Neuhäuser
author_facet	Jochen Menz Tim Range Johannes Trini Uwe Ludewig Benjamin Neuhäuser
author_sort	Jochen Menz
title	Molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions
title_short	Molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions
title_full	Molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions
title_fullStr	Molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions
title_full_unstemmed	Molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions
title_sort	molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting arabidopsis accessions
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fd866653f79843c18c4ec0ef3c9f83d0
work_keys_str_mv	AT jochenmenz molecularbasisofdifferentialnitrogenuseefficienciesandnitrogensourcepreferencesincontrastingarabidopsisaccessions AT timrange molecularbasisofdifferentialnitrogenuseefficienciesandnitrogensourcepreferencesincontrastingarabidopsisaccessions AT johannestrini molecularbasisofdifferentialnitrogenuseefficienciesandnitrogensourcepreferencesincontrastingarabidopsisaccessions AT uweludewig molecularbasisofdifferentialnitrogenuseefficienciesandnitrogensourcepreferencesincontrastingarabidopsisaccessions AT benjaminneuhauser molecularbasisofdifferentialnitrogenuseefficienciesandnitrogensourcepreferencesincontrastingarabidopsisaccessions
_version_	1718388229545132032

Molecular basis of differential nitrogen use efficiencies and nitrogen source preferences in contrasting Arabidopsis accessions

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