Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles

Megumi Fujiwara,1,2 Tomohiro Yonezawa,3 Toshiro Arai,1 Ichiro Yamamoto,1 Hiromichi Ohtsuka21Laboratory of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Tokyo, Japan; 2Laboratory of Large Animal Internal Medicine, 3Laboratory of Veterinary Physiology, Kitasato University, To...

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Autores principales:	Ohtsuka H, Yamamoto I, Arai T, Fujiwara M, Yonezawa T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/fd868c5aa85c4b23b61427d7688fcef3
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spelling	oai:doaj.org-article:fd868c5aa85c4b23b61427d7688fcef32021-12-02T11:42:17ZAlterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles2230-2034https://doaj.org/article/fd868c5aa85c4b23b61427d7688fcef32012-08-01T00:00:00Zhttp://www.dovepress.com/alterations-with-age-in-peripheral-blood-lymphocyte-subpopulations-and-a10831https://doaj.org/toc/2230-2034Megumi Fujiwara,1,2 Tomohiro Yonezawa,3 Toshiro Arai,1 Ichiro Yamamoto,1 Hiromichi Ohtsuka21Laboratory of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Tokyo, Japan; 2Laboratory of Large Animal Internal Medicine, 3Laboratory of Veterinary Physiology, Kitasato University, Towada, JapanPurpose: The immune system is considered to be affected by aging, which is linked to various immune pathogeneses. The purpose of this study was to determine age-associated changes in immune function of healthy dogs (beagles), specifically those of naive and memory T lymphocytes, based on cytokine synthesis.Patients and methods: Blood samples were obtained from 44 healthy beagles that were divided into three age-groups: young (&lt;4 years), middle-aged (4&ndash;8 years), and older dogs (&gt;8 years). Subpopulations of T lymphocytes were determined by flow cytometry. Transcriptional (mRNA) levels of cytokines were determined for primary-cultured leukocytes using quantitative real-time polymerase chain reaction.Results: There were negative correlations between dogs&rsquo;ages and the number of peripheral blood mononuclear cells, T cells, and B cells. In particular, the number of naive CD4+ CD45RA+ T cells and CD8+ CD45RA+ T cells significantly decreased with age. The mRNA levels for interleukin (IL)-2, IL-2R&alpha;, and interferon-gamma were significantly higher in young or middle-aged dogs (P &lt; 0.05), whereas IL-4 mRNA expression was not significantly different over the different age-groups. IL-2R&gamma; mRNA expression tended to decrease with age.Conclusion: Decreases of naive CD4+ and naive CD8+ T cells may be related to age-related immunosenescence in dogs. With regard to cytokine production, leukocyte IL-4 and IL-10 mRNA levels did not change with age, whereas IL-2, IL-2R&alpha;, and IL-2R&gamma; mRNA levels decreased with age. These altered cytokine mRNA expression patterns may contribute to decreased naive T-cell function(s) with aging.Keywords: aging, leukocyte subpopulation, cytokine, dog, naive T cell, IL-2ROhtsuka HYamamoto IArai TFujiwara MYonezawa TDove Medical PressarticleVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol 2012, Iss default, Pp 79-84 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Veterinary medicine SF600-1100
spellingShingle	Veterinary medicine SF600-1100 Ohtsuka H Yamamoto I Arai T Fujiwara M Yonezawa T Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles
description	Megumi Fujiwara,1,2 Tomohiro Yonezawa,3 Toshiro Arai,1 Ichiro Yamamoto,1 Hiromichi Ohtsuka21Laboratory of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Tokyo, Japan; 2Laboratory of Large Animal Internal Medicine, 3Laboratory of Veterinary Physiology, Kitasato University, Towada, JapanPurpose: The immune system is considered to be affected by aging, which is linked to various immune pathogeneses. The purpose of this study was to determine age-associated changes in immune function of healthy dogs (beagles), specifically those of naive and memory T lymphocytes, based on cytokine synthesis.Patients and methods: Blood samples were obtained from 44 healthy beagles that were divided into three age-groups: young (&lt;4 years), middle-aged (4&ndash;8 years), and older dogs (&gt;8 years). Subpopulations of T lymphocytes were determined by flow cytometry. Transcriptional (mRNA) levels of cytokines were determined for primary-cultured leukocytes using quantitative real-time polymerase chain reaction.Results: There were negative correlations between dogs&rsquo;ages and the number of peripheral blood mononuclear cells, T cells, and B cells. In particular, the number of naive CD4+ CD45RA+ T cells and CD8+ CD45RA+ T cells significantly decreased with age. The mRNA levels for interleukin (IL)-2, IL-2R&alpha;, and interferon-gamma were significantly higher in young or middle-aged dogs (P &lt; 0.05), whereas IL-4 mRNA expression was not significantly different over the different age-groups. IL-2R&gamma; mRNA expression tended to decrease with age.Conclusion: Decreases of naive CD4+ and naive CD8+ T cells may be related to age-related immunosenescence in dogs. With regard to cytokine production, leukocyte IL-4 and IL-10 mRNA levels did not change with age, whereas IL-2, IL-2R&alpha;, and IL-2R&gamma; mRNA levels decreased with age. These altered cytokine mRNA expression patterns may contribute to decreased naive T-cell function(s) with aging.Keywords: aging, leukocyte subpopulation, cytokine, dog, naive T cell, IL-2R
format	article
author	Ohtsuka H Yamamoto I Arai T Fujiwara M Yonezawa T
author_facet	Ohtsuka H Yamamoto I Arai T Fujiwara M Yonezawa T
author_sort	Ohtsuka H
title	Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles
title_short	Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles
title_full	Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles
title_fullStr	Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles
title_full_unstemmed	Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles
title_sort	alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/fd868c5aa85c4b23b61427d7688fcef3
work_keys_str_mv	AT ohtsukah alterationswithageinperipheralbloodlymphocytesubpopulationsandcytokinesynthesisinbeagles AT yamamotoi alterationswithageinperipheralbloodlymphocytesubpopulationsandcytokinesynthesisinbeagles AT arait alterationswithageinperipheralbloodlymphocytesubpopulationsandcytokinesynthesisinbeagles AT fujiwaram alterationswithageinperipheralbloodlymphocytesubpopulationsandcytokinesynthesisinbeagles AT yonezawat alterationswithageinperipheralbloodlymphocytesubpopulationsandcytokinesynthesisinbeagles
_version_	1718395379556286464

Alterations with age in peripheral blood lymphocyte subpopulations and cytokine synthesis in beagles

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