Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass

Abstract Thermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, and from Sargassum sp. biomass, is studied in this work in batch assays and also in a continuous reactor experiment. Pursuing the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	R. M. Silva, A. A. Abreu, A. F. Salvador, M. M. Alves, I. C. Neves, M. A. Pereira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd8c2a4e716f42b69d5505856a90134e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fd8c2a4e716f42b69d5505856a90134e
record_format	dspace
spelling	oai:doaj.org-article:fd8c2a4e716f42b69d5505856a90134e2021-12-02T15:07:54ZZeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass10.1038/s41598-021-95615-12045-2322https://doaj.org/article/fd8c2a4e716f42b69d5505856a90134e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95615-1https://doaj.org/toc/2045-2322Abstract Thermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, and from Sargassum sp. biomass, is studied in this work in batch assays and also in a continuous reactor experiment. Pursuing the interest of studying interactions between inorganic materials (adsorbents, conductive and others) and anaerobic bacteria, the biological processes were amended with variable amounts of a zeolite type-13X in the range of zeolite/inoculum (in VS) ratios (Z/I) of 0.065–0.26 g g−1. In the batch assays, the presence of the zeolite was beneficial to increase the hydrogen titer by 15–21% with C5 and C6-sugars as compared to the control, and an increase of 27% was observed in the batch fermentation of Sargassum sp. Hydrogen yields also increased by 10–26% with sugars in the presence of the zeolite. The rate of hydrogen production increased linearly with the Z/I ratios in the experiments with C5 and C6-sugars. In the batch assay with Sargassum sp., there was an optimum value of Z/I of 0.13 g g−1 where the H2 production rate observed was the highest, although all values were in a narrow range between 3.21 and 4.19 mmol L−1 day−1. The positive effect of the zeolite was also observed in a continuous high-rate reactor fed with C5 and C6-sugars. The increase of the organic loading rate (OLR) from 8.8 to 17.6 kg m−3 day−1 of COD led to lower hydrogen production rates but, upon zeolite addition (0.26 g g−1 VS inoculum), the hydrogen production increased significantly from 143 to 413 mL L−1 day−1. Interestingly, the presence of zeolite in the continuous operation had a remarkable impact in the microbial community and in the profile of fermentation products. The effect of zeolite could be related to several properties, including the porous structure and the associated surface area available for bacterial adhesion, potential release of trace elements, ion-exchanger capacity or ability to adsorb different compounds (i.e. protons). The observations opens novel perspectives and will stimulate further research not only in biohydrogen production, but broadly in the field of interactions between bacteria and inorganic materials.R. M. SilvaA. A. AbreuA. F. SalvadorM. M. AlvesI. C. NevesM. A. PereiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q R. M. Silva A. A. Abreu A. F. Salvador M. M. Alves I. C. Neves M. A. Pereira Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass
description	Abstract Thermophilic biohydrogen production by dark fermentation from a mixture (1:1) of C5 (arabinose) and C6 (glucose) sugars, present in lignocellulosic hydrolysates, and from Sargassum sp. biomass, is studied in this work in batch assays and also in a continuous reactor experiment. Pursuing the interest of studying interactions between inorganic materials (adsorbents, conductive and others) and anaerobic bacteria, the biological processes were amended with variable amounts of a zeolite type-13X in the range of zeolite/inoculum (in VS) ratios (Z/I) of 0.065–0.26 g g−1. In the batch assays, the presence of the zeolite was beneficial to increase the hydrogen titer by 15–21% with C5 and C6-sugars as compared to the control, and an increase of 27% was observed in the batch fermentation of Sargassum sp. Hydrogen yields also increased by 10–26% with sugars in the presence of the zeolite. The rate of hydrogen production increased linearly with the Z/I ratios in the experiments with C5 and C6-sugars. In the batch assay with Sargassum sp., there was an optimum value of Z/I of 0.13 g g−1 where the H2 production rate observed was the highest, although all values were in a narrow range between 3.21 and 4.19 mmol L−1 day−1. The positive effect of the zeolite was also observed in a continuous high-rate reactor fed with C5 and C6-sugars. The increase of the organic loading rate (OLR) from 8.8 to 17.6 kg m−3 day−1 of COD led to lower hydrogen production rates but, upon zeolite addition (0.26 g g−1 VS inoculum), the hydrogen production increased significantly from 143 to 413 mL L−1 day−1. Interestingly, the presence of zeolite in the continuous operation had a remarkable impact in the microbial community and in the profile of fermentation products. The effect of zeolite could be related to several properties, including the porous structure and the associated surface area available for bacterial adhesion, potential release of trace elements, ion-exchanger capacity or ability to adsorb different compounds (i.e. protons). The observations opens novel perspectives and will stimulate further research not only in biohydrogen production, but broadly in the field of interactions between bacteria and inorganic materials.
format	article
author	R. M. Silva A. A. Abreu A. F. Salvador M. M. Alves I. C. Neves M. A. Pereira
author_facet	R. M. Silva A. A. Abreu A. F. Salvador M. M. Alves I. C. Neves M. A. Pereira
author_sort	R. M. Silva
title	Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass
title_short	Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass
title_full	Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass
title_fullStr	Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass
title_full_unstemmed	Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass
title_sort	zeolite addition to improve biohydrogen production from dark fermentation of c5/c6-sugars and sargassum sp. biomass
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fd8c2a4e716f42b69d5505856a90134e
work_keys_str_mv	AT rmsilva zeoliteadditiontoimprovebiohydrogenproductionfromdarkfermentationofc5c6sugarsandsargassumspbiomass AT aaabreu zeoliteadditiontoimprovebiohydrogenproductionfromdarkfermentationofc5c6sugarsandsargassumspbiomass AT afsalvador zeoliteadditiontoimprovebiohydrogenproductionfromdarkfermentationofc5c6sugarsandsargassumspbiomass AT mmalves zeoliteadditiontoimprovebiohydrogenproductionfromdarkfermentationofc5c6sugarsandsargassumspbiomass AT icneves zeoliteadditiontoimprovebiohydrogenproductionfromdarkfermentationofc5c6sugarsandsargassumspbiomass AT mapereira zeoliteadditiontoimprovebiohydrogenproductionfromdarkfermentationofc5c6sugarsandsargassumspbiomass
_version_	1718388361215868928

Zeolite addition to improve biohydrogen production from dark fermentation of C5/C6-sugars and Sargassum sp. biomass

Ejemplares similares