Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation

Abstract We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The...

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Autores principales:	Katerina V. Volodina, David Avnir, Vladimir V. Vinogradov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd91f9baccb945a0acdf32300f0d59fd
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spelling	oai:doaj.org-article:fd91f9baccb945a0acdf32300f0d59fd2021-12-02T11:52:22ZAlumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation10.1038/s41598-017-01436-62045-2322https://doaj.org/article/fd91f9baccb945a0acdf32300f0d59fd2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01436-6https://doaj.org/toc/2045-2322Abstract We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method, demonstrated by the renaturation of several enzymes, is efficient, rapid, employs a minimal amount of reagents and even can be applied to renature mixture of the denatured enzymes.Katerina V. VolodinaDavid AvnirVladimir V. VinogradovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katerina V. Volodina David Avnir Vladimir V. Vinogradov Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation
description	Abstract We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method, demonstrated by the renaturation of several enzymes, is efficient, rapid, employs a minimal amount of reagents and even can be applied to renature mixture of the denatured enzymes.
format	article
author	Katerina V. Volodina David Avnir Vladimir V. Vinogradov
author_facet	Katerina V. Volodina David Avnir Vladimir V. Vinogradov
author_sort	Katerina V. Volodina
title	Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation
title_short	Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation
title_full	Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation
title_fullStr	Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation
title_full_unstemmed	Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation
title_sort	alumina nanoparticle-assisted enzyme refolding: a versatile methodology for proteins renaturation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fd91f9baccb945a0acdf32300f0d59fd
work_keys_str_mv	AT katerinavvolodina aluminananoparticleassistedenzymerefoldingaversatilemethodologyforproteinsrenaturation AT davidavnir aluminananoparticleassistedenzymerefoldingaversatilemethodologyforproteinsrenaturation AT vladimirvvinogradov aluminananoparticleassistedenzymerefoldingaversatilemethodologyforproteinsrenaturation
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Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation

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