The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.

RacA is the main Rho GTPase in Aspergillus niger regulating polarity maintenance via controlling actin dynamics. Both deletion and dominant activation of RacA (Rac(G18V)) provoke an actin localization defect and thereby loss of polarized tip extension, resulting in frequent dichotomous branching in...

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Autores principales:	Min Jin Kwon, Benjamin M Nitsche, Mark Arentshorst, Thomas R Jørgensen, Arthur F J Ram, Vera Meyer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd9347551bcf463fb43a9d9e8bfbdd40
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spelling	oai:doaj.org-article:fd9347551bcf463fb43a9d9e8bfbdd402021-11-18T09:03:11ZThe transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.1932-620310.1371/journal.pone.0068946https://doaj.org/article/fd9347551bcf463fb43a9d9e8bfbdd402013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23894378/?tool=EBIhttps://doaj.org/toc/1932-6203RacA is the main Rho GTPase in Aspergillus niger regulating polarity maintenance via controlling actin dynamics. Both deletion and dominant activation of RacA (Rac(G18V)) provoke an actin localization defect and thereby loss of polarized tip extension, resulting in frequent dichotomous branching in the ΔracA strain and an apolar growing phenotype for Rac(G18V). In the current study the transcriptomics and physiological consequences of these morphological changes were investigated and compared with the data of the morphogenetic network model for the dichotomous branching mutant ramosa-1. This integrated approach revealed that polar tip growth is most likely orchestrated by the concerted activities of phospholipid signaling, sphingolipid signaling, TORC2 signaling, calcium signaling and CWI signaling pathways. The transcriptomic signatures and the reconstructed network model for all three morphology mutants (ΔracA, Rac(G18V), ramosa-1) imply that these pathways become integrated to bring about different physiological adaptations including changes in sterol, zinc and amino acid metabolism and changes in ion transport and protein trafficking. Finally, the fate of exocytotic (SncA) and endocytotic (AbpA, SlaB) markers in the dichotomous branching mutant ΔracA was followed, demonstrating that hyperbranching does not per se result in increased protein secretion.Min Jin KwonBenjamin M NitscheMark ArentshorstThomas R JørgensenArthur F J RamVera MeyerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68946 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Min Jin Kwon Benjamin M Nitsche Mark Arentshorst Thomas R Jørgensen Arthur F J Ram Vera Meyer The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.
description	RacA is the main Rho GTPase in Aspergillus niger regulating polarity maintenance via controlling actin dynamics. Both deletion and dominant activation of RacA (Rac(G18V)) provoke an actin localization defect and thereby loss of polarized tip extension, resulting in frequent dichotomous branching in the ΔracA strain and an apolar growing phenotype for Rac(G18V). In the current study the transcriptomics and physiological consequences of these morphological changes were investigated and compared with the data of the morphogenetic network model for the dichotomous branching mutant ramosa-1. This integrated approach revealed that polar tip growth is most likely orchestrated by the concerted activities of phospholipid signaling, sphingolipid signaling, TORC2 signaling, calcium signaling and CWI signaling pathways. The transcriptomic signatures and the reconstructed network model for all three morphology mutants (ΔracA, Rac(G18V), ramosa-1) imply that these pathways become integrated to bring about different physiological adaptations including changes in sterol, zinc and amino acid metabolism and changes in ion transport and protein trafficking. Finally, the fate of exocytotic (SncA) and endocytotic (AbpA, SlaB) markers in the dichotomous branching mutant ΔracA was followed, demonstrating that hyperbranching does not per se result in increased protein secretion.
format	article
author	Min Jin Kwon Benjamin M Nitsche Mark Arentshorst Thomas R Jørgensen Arthur F J Ram Vera Meyer
author_facet	Min Jin Kwon Benjamin M Nitsche Mark Arentshorst Thomas R Jørgensen Arthur F J Ram Vera Meyer
author_sort	Min Jin Kwon
title	The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.
title_short	The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.
title_full	The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.
title_fullStr	The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.
title_full_unstemmed	The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.
title_sort	transcriptomic signature of raca activation and inactivation provides new insights into the morphogenetic network of aspergillus niger.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/fd9347551bcf463fb43a9d9e8bfbdd40
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The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.

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