The intestinal tuft cell nanostructure in 3D

Abstract Once referred to as “peculiar,” tuft cells are enigmatic epithelial cells. Here, we reasoned that future functional studies could be derived from a complete account of the tuft cell ultrastructure. We identified and documented the volumetric ultrastructure at nanometer resolution (4–5 nm/pi...

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Autores principales:	Ben Hoover, Valentina Baena, Melanie M. Kaelberer, Feven Getaneh, Skarleth Chinchilla, Diego V. Bohórquez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd942acf13664eab84ba5db6ac51b3f2
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spelling	oai:doaj.org-article:fd942acf13664eab84ba5db6ac51b3f22021-12-02T12:32:20ZThe intestinal tuft cell nanostructure in 3D10.1038/s41598-017-01520-x2045-2322https://doaj.org/article/fd942acf13664eab84ba5db6ac51b3f22017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01520-xhttps://doaj.org/toc/2045-2322Abstract Once referred to as “peculiar,” tuft cells are enigmatic epithelial cells. Here, we reasoned that future functional studies could be derived from a complete account of the tuft cell ultrastructure. We identified and documented the volumetric ultrastructure at nanometer resolution (4–5 nm/pixel) of specific intestinal tuft cells. The techniques used were Serial Block-Face (SBF) and Automated Tape-collecting Ultra-Microtome (ATUM) Scanning Electron Microscopy (SEM). Our results exposed a short (~15 µm) basal cytoplasmic process devoid of secretory vesicles. Volume rendering of serial sections unveiled several thin cytospinules (~1 µm). These cytospinules project from the tuft cell into the nuclei of neighboring epithelial cells. Volume rendering also revealed within the tuft cell an elegant network of interconnected tubules. The network forms a passage from the base of the microvilli to the rough endoplasmic reticulum. Based on their location and microanatomy, the tuft cells’ cytospinules, and tubular network, might facilitate the exchange of molecular cargo with nuclei of neighboring cells, and the gut lumen.Ben HooverValentina BaenaMelanie M. KaelbererFeven GetanehSkarleth ChinchillaDiego V. BohórquezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ben Hoover Valentina Baena Melanie M. Kaelberer Feven Getaneh Skarleth Chinchilla Diego V. Bohórquez The intestinal tuft cell nanostructure in 3D
description	Abstract Once referred to as “peculiar,” tuft cells are enigmatic epithelial cells. Here, we reasoned that future functional studies could be derived from a complete account of the tuft cell ultrastructure. We identified and documented the volumetric ultrastructure at nanometer resolution (4–5 nm/pixel) of specific intestinal tuft cells. The techniques used were Serial Block-Face (SBF) and Automated Tape-collecting Ultra-Microtome (ATUM) Scanning Electron Microscopy (SEM). Our results exposed a short (~15 µm) basal cytoplasmic process devoid of secretory vesicles. Volume rendering of serial sections unveiled several thin cytospinules (~1 µm). These cytospinules project from the tuft cell into the nuclei of neighboring epithelial cells. Volume rendering also revealed within the tuft cell an elegant network of interconnected tubules. The network forms a passage from the base of the microvilli to the rough endoplasmic reticulum. Based on their location and microanatomy, the tuft cells’ cytospinules, and tubular network, might facilitate the exchange of molecular cargo with nuclei of neighboring cells, and the gut lumen.
format	article
author	Ben Hoover Valentina Baena Melanie M. Kaelberer Feven Getaneh Skarleth Chinchilla Diego V. Bohórquez
author_facet	Ben Hoover Valentina Baena Melanie M. Kaelberer Feven Getaneh Skarleth Chinchilla Diego V. Bohórquez
author_sort	Ben Hoover
title	The intestinal tuft cell nanostructure in 3D
title_short	The intestinal tuft cell nanostructure in 3D
title_full	The intestinal tuft cell nanostructure in 3D
title_fullStr	The intestinal tuft cell nanostructure in 3D
title_full_unstemmed	The intestinal tuft cell nanostructure in 3D
title_sort	intestinal tuft cell nanostructure in 3d
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fd942acf13664eab84ba5db6ac51b3f2
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The intestinal tuft cell nanostructure in 3D

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