Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process

Among 200 fungal strains isolated from the soil, only one culture filtrate of Aspergillus flavus JCK-4087 showed strong nematicidal activity against Meloidogyne incognita. The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JC...

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Autores principales:	Van Thi Nguyen, Nan Hee Yu, Yookyung Lee, In Min Hwang, Hung Xuan Bui, Jin-Cheol Kim
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cyclopiazonic acid nematicidal activity Plackett–Burman central composite design response surface methodology root-knot nematode Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fd9a5116f7db45e693dc972a9020de05
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spelling	oai:doaj.org-article:fd9a5116f7db45e693dc972a9020de052021-11-30T17:12:25ZNematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process1664-302X10.3389/fmicb.2021.726504https://doaj.org/article/fd9a5116f7db45e693dc972a9020de052021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.726504/fullhttps://doaj.org/toc/1664-302XAmong 200 fungal strains isolated from the soil, only one culture filtrate of Aspergillus flavus JCK-4087 showed strong nematicidal activity against Meloidogyne incognita. The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JCK-4087 also produced aflatoxins, six strains of Penicillium commune, which have been reported to be CPA producers, were obtained from the bank and then tested for their CPA productivity. CPA was isolated from the culture filtrate of P. commune KACC 45973. CPA killed the second-stage juveniles of M. incognita, M. hapla, and M. arearia with EC50–3 days 4.50, 18.82, and 60.51 μg mL–1, respectively. CPA also significantly inhibited egg hatch of M. incognita and M. hapla after a total of 28 days of treatment with the concentrations > 25 μg mL–1. The enhancement of CPA production by P. commune KACC 45973 was explored using an optimized medium based on Plackett–Burman design (PBD) and central composite design (CCD). The highest CPA production (381.48 μg mL–1) was obtained from the optimized medium, exhibiting an increase of 7.88 times when compared with that from potato dextrose broth culture. Application of the wettable power-type formulation of the ethyl acetate extract of the culture filtrate of KACC 45973 reduced gall formation and nematode populations in tomato roots and soils under greenhouse conditions. These results suggest that CPA produced by P. commune KACC 45973 can be used as either a biochemical nematicide or a lead molecule for developing chemical nematicides to control root-knot nematodes.Van Thi NguyenNan Hee YuYookyung LeeIn Min HwangHung Xuan BuiJin-Cheol KimFrontiers Media S.A.articlecyclopiazonic acidnematicidal activityPlackett–Burmancentral composite designresponse surface methodologyroot-knot nematodeMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyclopiazonic acid nematicidal activity Plackett–Burman central composite design response surface methodology root-knot nematode Microbiology QR1-502
spellingShingle	cyclopiazonic acid nematicidal activity Plackett–Burman central composite design response surface methodology root-knot nematode Microbiology QR1-502 Van Thi Nguyen Nan Hee Yu Yookyung Lee In Min Hwang Hung Xuan Bui Jin-Cheol Kim Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process
description	Among 200 fungal strains isolated from the soil, only one culture filtrate of Aspergillus flavus JCK-4087 showed strong nematicidal activity against Meloidogyne incognita. The nematicidal metabolite isolated from the culture filtrate of JCK-4087 was identified as cyclopiazonic acid (CPA). Because JCK-4087 also produced aflatoxins, six strains of Penicillium commune, which have been reported to be CPA producers, were obtained from the bank and then tested for their CPA productivity. CPA was isolated from the culture filtrate of P. commune KACC 45973. CPA killed the second-stage juveniles of M. incognita, M. hapla, and M. arearia with EC50–3 days 4.50, 18.82, and 60.51 μg mL–1, respectively. CPA also significantly inhibited egg hatch of M. incognita and M. hapla after a total of 28 days of treatment with the concentrations > 25 μg mL–1. The enhancement of CPA production by P. commune KACC 45973 was explored using an optimized medium based on Plackett–Burman design (PBD) and central composite design (CCD). The highest CPA production (381.48 μg mL–1) was obtained from the optimized medium, exhibiting an increase of 7.88 times when compared with that from potato dextrose broth culture. Application of the wettable power-type formulation of the ethyl acetate extract of the culture filtrate of KACC 45973 reduced gall formation and nematode populations in tomato roots and soils under greenhouse conditions. These results suggest that CPA produced by P. commune KACC 45973 can be used as either a biochemical nematicide or a lead molecule for developing chemical nematicides to control root-knot nematodes.
format	article
author	Van Thi Nguyen Nan Hee Yu Yookyung Lee In Min Hwang Hung Xuan Bui Jin-Cheol Kim
author_facet	Van Thi Nguyen Nan Hee Yu Yookyung Lee In Min Hwang Hung Xuan Bui Jin-Cheol Kim
author_sort	Van Thi Nguyen
title	Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process
title_short	Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process
title_full	Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process
title_fullStr	Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process
title_full_unstemmed	Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process
title_sort	nematicidal activity of cyclopiazonic acid derived from penicillium commune against root-knot nematodes and optimization of the culture fermentation process
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fd9a5116f7db45e693dc972a9020de05
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Nematicidal Activity of Cyclopiazonic Acid Derived From Penicillium commune Against Root-Knot Nematodes and Optimization of the Culture Fermentation Process

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