Roles of miR-210 in the pathogenesis of pre-eclampsia

Introduction This study aimed to explore the bio-function of miR-210 in the pathogenesis of pre-eclampsia and provide new insights into the diagnosis and treatment of pre-eclampsia. Material and methods A JAR cell line cultured in standard or hypoxic conditions was used in this study. Expression le...

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Autores principales:	Jiyun Li, Guimei Wu, Yanmin Cao, Zhi Hou
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2018
Materias:	invasion pre-eclampsia microrna210 mir-210 ptpn2 migration Medicine R
Acceso en línea:	https://doaj.org/article/fda2905d4f3d4946b33d00018615042d
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spelling	oai:doaj.org-article:fda2905d4f3d4946b33d00018615042d2021-12-02T19:15:49ZRoles of miR-210 in the pathogenesis of pre-eclampsia1734-19221896-915110.5114/aoms.2018.73129https://doaj.org/article/fda2905d4f3d4946b33d00018615042d2018-12-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Roles-of-miR-210-in-the-pathogenesis-of-pre-eclampsia,81389,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction This study aimed to explore the bio-function of miR-210 in the pathogenesis of pre-eclampsia and provide new insights into the diagnosis and treatment of pre-eclampsia. Material and methods A JAR cell line cultured in standard or hypoxic conditions was used in this study. Expression levels of miR-210 and PTPN2 were determined using real-time polymerase chain reaction (RT-PCR). Protein and phosphorylation levels were assessed using western blotting. Proliferation of JAR cells was evaluated using MTT assay. Migration and invasion were measured using transwell assay. Results Expression of miR-210 increased significantly in a time-dependent manner after hypoxia treatment within 36 h (p < 0.05). miR-210 inhibitor significantly decreased the cell proliferation, migration, and invasion (p < 0.05), while miR-210 mimic reversed these findings (p < 0.05). Hypoxia significantly suppressed the expression of PTPN2; however, this elevation was abolished by miR-210 inhibitor (p < 0.05). Inhibition of PTPN2 or hypoxia significantly increased the proliferation, migration, and invasion of JAR cells, while miR-210 inhibitor significantly reversed these changes (p < 0.05). The phosphorylation levels of PDGFR, Akt, and Erk were markedly upregulated by hypoxia or si-PTPN2, but this effect was abolished by miR-210 inhibitor (p < 0.05). Conclusions miR-210 can promote proliferation, migration, and invasion via downregulating PTPN2 in the PDGFR-Akt pathwayJiyun LiGuimei WuYanmin CaoZhi HouTermedia Publishing Housearticleinvasionpre-eclampsiamicrorna210pre-eclampsiamir-210ptpn2invasionmigrationMedicineRENArchives of Medical Science, Vol 15, Iss 1, Pp 183-190 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	invasion pre-eclampsia microrna210 pre-eclampsia mir-210 ptpn2 invasion migration Medicine R
spellingShingle	invasion pre-eclampsia microrna210 pre-eclampsia mir-210 ptpn2 invasion migration Medicine R Jiyun Li Guimei Wu Yanmin Cao Zhi Hou Roles of miR-210 in the pathogenesis of pre-eclampsia
description	Introduction This study aimed to explore the bio-function of miR-210 in the pathogenesis of pre-eclampsia and provide new insights into the diagnosis and treatment of pre-eclampsia. Material and methods A JAR cell line cultured in standard or hypoxic conditions was used in this study. Expression levels of miR-210 and PTPN2 were determined using real-time polymerase chain reaction (RT-PCR). Protein and phosphorylation levels were assessed using western blotting. Proliferation of JAR cells was evaluated using MTT assay. Migration and invasion were measured using transwell assay. Results Expression of miR-210 increased significantly in a time-dependent manner after hypoxia treatment within 36 h (p < 0.05). miR-210 inhibitor significantly decreased the cell proliferation, migration, and invasion (p < 0.05), while miR-210 mimic reversed these findings (p < 0.05). Hypoxia significantly suppressed the expression of PTPN2; however, this elevation was abolished by miR-210 inhibitor (p < 0.05). Inhibition of PTPN2 or hypoxia significantly increased the proliferation, migration, and invasion of JAR cells, while miR-210 inhibitor significantly reversed these changes (p < 0.05). The phosphorylation levels of PDGFR, Akt, and Erk were markedly upregulated by hypoxia or si-PTPN2, but this effect was abolished by miR-210 inhibitor (p < 0.05). Conclusions miR-210 can promote proliferation, migration, and invasion via downregulating PTPN2 in the PDGFR-Akt pathway
format	article
author	Jiyun Li Guimei Wu Yanmin Cao Zhi Hou
author_facet	Jiyun Li Guimei Wu Yanmin Cao Zhi Hou
author_sort	Jiyun Li
title	Roles of miR-210 in the pathogenesis of pre-eclampsia
title_short	Roles of miR-210 in the pathogenesis of pre-eclampsia
title_full	Roles of miR-210 in the pathogenesis of pre-eclampsia
title_fullStr	Roles of miR-210 in the pathogenesis of pre-eclampsia
title_full_unstemmed	Roles of miR-210 in the pathogenesis of pre-eclampsia
title_sort	roles of mir-210 in the pathogenesis of pre-eclampsia
publisher	Termedia Publishing House
publishDate	2018
url	https://doaj.org/article/fda2905d4f3d4946b33d00018615042d
work_keys_str_mv	AT jiyunli rolesofmir210inthepathogenesisofpreeclampsia AT guimeiwu rolesofmir210inthepathogenesisofpreeclampsia AT yanmincao rolesofmir210inthepathogenesisofpreeclampsia AT zhihou rolesofmir210inthepathogenesisofpreeclampsia
_version_	1718377009472602112

Roles of miR-210 in the pathogenesis of pre-eclampsia

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