Role of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia

We determined three isoforms of APOEprotein differing by amino acid residues in 112 and 158 positions: aroE2 (112Cys, 158Cys), apoE3 (112Cys, 158Arg) and aroE4 (112Arg, 158Arg). Protein isoforms differed in the degree of effectiveness of protein interaction with the liver cell receptors during chole...

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Autores principales:	O. V. Kalyuzhnaya, T. A. Bairova, L. V. Rychkova, L. I. Kolesnikova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	apolipoprotein e genotype russian lipids teenagers Science Q
Acceso en línea:	https://doaj.org/article/fda411e13b684e6bbd7f63e41899d5e7
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spelling	oai:doaj.org-article:fda411e13b684e6bbd7f63e41899d5e72021-11-23T06:14:34ZRole of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia2541-94202587-959610.12737/article_590823a3eb6185.48833230https://doaj.org/article/fda411e13b684e6bbd7f63e41899d5e72016-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/208https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596We determined three isoforms of APOEprotein differing by amino acid residues in 112 and 158 positions: aroE2 (112Cys, 158Cys), apoE3 (112Cys, 158Arg) and aroE4 (112Arg, 158Arg). Protein isoforms differed in the degree of effectiveness of protein interaction with the liver cell receptors during cholesterol transport. We studied the prevalence of genotypes and alleles of polymorphic variant ε2/ε3/ε4 of apolipoprotein E gene in a group of healthy Russian adolescents living on a territory of Eastern Siberia (Irkutsk region). 73 healthy Russian adolescents (13-18 years) were included in the study. A biochemical analysis of blood lipids was carried out for each adolescent. We also genotyped the carriage of genotypes and alleles of polymorphic variant ε2/ε3/ε4 of apolipoprotein E gene. In the study the mostfrequently met genotypes were: ε3/ε3 in 57.5 % of cases, the genotype ε2/ε3 -16.4 %, ε3/ε4 -19.2 %, ε2/ε4 - 82.7 %, ε4/ε4 - 4.1 %. APOE gene genotype distribution was consistent with Hardy - Weinberg equilibrium in the studied sample (p = 0.498). The analysis of the allele and genotype distribution of polymorphic variants ε2/ε3/ε4 of APOE gene, and comparison with the previous population-based data showed that the frequency of alleles and genotypes ε2/ε3 ε4 APOE gene in the studied sample of adolescents corresponds to frequencies in the previously studied samples of the Caucasians. Comparison of lipid carriers between genotypes in the studied sample of adolescents showed no connection of genotypes with the level of blood lipid profile.O. V. KalyuzhnayaT. A. BairovaL. V. RychkovaL. I. KolesnikovaScientific Сentre for Family Health and Human Reproduction Problemsarticleapolipoprotein egenotyperussianlipidsteenagersScienceQRUActa Biomedica Scientifica, Vol 1, Iss 3(2), Pp 145-149 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	apolipoprotein e genotype russian lipids teenagers Science Q
spellingShingle	apolipoprotein e genotype russian lipids teenagers Science Q O. V. Kalyuzhnaya T. A. Bairova L. V. Rychkova L. I. Kolesnikova Role of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia
description	We determined three isoforms of APOEprotein differing by amino acid residues in 112 and 158 positions: aroE2 (112Cys, 158Cys), apoE3 (112Cys, 158Arg) and aroE4 (112Arg, 158Arg). Protein isoforms differed in the degree of effectiveness of protein interaction with the liver cell receptors during cholesterol transport. We studied the prevalence of genotypes and alleles of polymorphic variant ε2/ε3/ε4 of apolipoprotein E gene in a group of healthy Russian adolescents living on a territory of Eastern Siberia (Irkutsk region). 73 healthy Russian adolescents (13-18 years) were included in the study. A biochemical analysis of blood lipids was carried out for each adolescent. We also genotyped the carriage of genotypes and alleles of polymorphic variant ε2/ε3/ε4 of apolipoprotein E gene. In the study the mostfrequently met genotypes were: ε3/ε3 in 57.5 % of cases, the genotype ε2/ε3 -16.4 %, ε3/ε4 -19.2 %, ε2/ε4 - 82.7 %, ε4/ε4 - 4.1 %. APOE gene genotype distribution was consistent with Hardy - Weinberg equilibrium in the studied sample (p = 0.498). The analysis of the allele and genotype distribution of polymorphic variants ε2/ε3/ε4 of APOE gene, and comparison with the previous population-based data showed that the frequency of alleles and genotypes ε2/ε3 ε4 APOE gene in the studied sample of adolescents corresponds to frequencies in the previously studied samples of the Caucasians. Comparison of lipid carriers between genotypes in the studied sample of adolescents showed no connection of genotypes with the level of blood lipid profile.
format	article
author	O. V. Kalyuzhnaya T. A. Bairova L. V. Rychkova L. I. Kolesnikova
author_facet	O. V. Kalyuzhnaya T. A. Bairova L. V. Rychkova L. I. Kolesnikova
author_sort	O. V. Kalyuzhnaya
title	Role of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia
title_short	Role of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia
title_full	Role of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia
title_fullStr	Role of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia
title_full_unstemmed	Role of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia
title_sort	role of e2/e3/e4 allelic polymorphism of apoli-poprotein e in regulation of lipid profile in caucasian population of eastern siberia
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/fda411e13b684e6bbd7f63e41899d5e7
work_keys_str_mv	AT ovkalyuzhnaya roleofe2e3e4allelicpolymorphismofapolipoproteineinregulationoflipidprofileincaucasianpopulationofeasternsiberia AT tabairova roleofe2e3e4allelicpolymorphismofapolipoproteineinregulationoflipidprofileincaucasianpopulationofeasternsiberia AT lvrychkova roleofe2e3e4allelicpolymorphismofapolipoproteineinregulationoflipidprofileincaucasianpopulationofeasternsiberia AT likolesnikova roleofe2e3e4allelicpolymorphismofapolipoproteineinregulationoflipidprofileincaucasianpopulationofeasternsiberia
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Role of e2/e3/e4 allelic polymorphism of apoli-poprotein E in regulation of lipid profile in Caucasian population of Eastern Siberia

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