Actin and myosin-dependent localization of mRNA to dendrites.

The localization of mRNAs within axons and dendrites allows neurons to manipulate protein levels in a time and location dependent manner and is essential for processes such as synaptic plasticity and axon guidance. However, an essential step in the process of mRNA localization, the decision to traff...

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Autores principales:	Varuzhan Balasanyan, Don B Arnold
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fda6c1dbc181463680bb28e6bb2832b6
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spelling	oai:doaj.org-article:fda6c1dbc181463680bb28e6bb2832b62021-11-18T08:27:47ZActin and myosin-dependent localization of mRNA to dendrites.1932-620310.1371/journal.pone.0092349https://doaj.org/article/fda6c1dbc181463680bb28e6bb2832b62014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24637809/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The localization of mRNAs within axons and dendrites allows neurons to manipulate protein levels in a time and location dependent manner and is essential for processes such as synaptic plasticity and axon guidance. However, an essential step in the process of mRNA localization, the decision to traffic to dendrites and/or axons, remains poorly understood. Here we show that Myosin Va and actin filaments are necessary for the dendritic localization of the mRNA binding protein Staufen 1 and of mRNA encoding the microtubule binding protein Map2. Blocking the function or expression of Myosin Va or depolymerizing actin filaments leads to localization of Staufen 1 and of Map2 mRNA in both axons and dendrites. Furthermore, interaction with Myosin Va plays an instructive role in the dendritic localization of Hermes 1, an RNA binding protein. Wild-type Hermes 1 localizes to both axons and dendrites, whereas Hermes 1 fused with a Myosin Va binding peptide, localizes specifically to dendrites. Thus, our results suggest that targeting of mRNAs to the dendrites is mediated by a mechanism that is dependent on actin and Myosin Va.Varuzhan BalasanyanDon B ArnoldPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92349 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Varuzhan Balasanyan Don B Arnold Actin and myosin-dependent localization of mRNA to dendrites.
description	The localization of mRNAs within axons and dendrites allows neurons to manipulate protein levels in a time and location dependent manner and is essential for processes such as synaptic plasticity and axon guidance. However, an essential step in the process of mRNA localization, the decision to traffic to dendrites and/or axons, remains poorly understood. Here we show that Myosin Va and actin filaments are necessary for the dendritic localization of the mRNA binding protein Staufen 1 and of mRNA encoding the microtubule binding protein Map2. Blocking the function or expression of Myosin Va or depolymerizing actin filaments leads to localization of Staufen 1 and of Map2 mRNA in both axons and dendrites. Furthermore, interaction with Myosin Va plays an instructive role in the dendritic localization of Hermes 1, an RNA binding protein. Wild-type Hermes 1 localizes to both axons and dendrites, whereas Hermes 1 fused with a Myosin Va binding peptide, localizes specifically to dendrites. Thus, our results suggest that targeting of mRNAs to the dendrites is mediated by a mechanism that is dependent on actin and Myosin Va.
format	article
author	Varuzhan Balasanyan Don B Arnold
author_facet	Varuzhan Balasanyan Don B Arnold
author_sort	Varuzhan Balasanyan
title	Actin and myosin-dependent localization of mRNA to dendrites.
title_short	Actin and myosin-dependent localization of mRNA to dendrites.
title_full	Actin and myosin-dependent localization of mRNA to dendrites.
title_fullStr	Actin and myosin-dependent localization of mRNA to dendrites.
title_full_unstemmed	Actin and myosin-dependent localization of mRNA to dendrites.
title_sort	actin and myosin-dependent localization of mrna to dendrites.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fda6c1dbc181463680bb28e6bb2832b6
work_keys_str_mv	AT varuzhanbalasanyan actinandmyosindependentlocalizationofmrnatodendrites AT donbarnold actinandmyosindependentlocalizationofmrnatodendrites
_version_	1718421780731789312

Actin and myosin-dependent localization of mRNA to dendrites.

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