Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats

Introduction: Reactive oxygen species (ROS) are the main factors in pathogenesis of renal ischemia-reperfusion (I/R) injury. Objectives: The aim of this study was to evaluate the renoprotective effect of rosmarinic acid (ROA) as an antioxidant substance against renal I/R injury. Materials and Method...

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Autores principales:	Majid Tavafi, Hassan Ahmadvand, Ahmad Tamjidipour, Afshin Hasanvand
Formato:	article
Lenguaje:	EN
Publicado:	Society of Diabetic Nephropathy Prevention 2020
Materias:	oxidative stress kidney antioxidant ischemia-reperfusion rosmarinic acid reactive oxygen species Therapeutics. Pharmacology RM1-950 Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/fda80b945c5d4e9f819a3d620c27c1f9
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spelling	oai:doaj.org-article:fda80b945c5d4e9f819a3d620c27c1f92021-11-17T08:13:31ZRosmarinic acid ameliorates renal ischemia reperfusion damage in rats2345-420210.34172/npj.2020.15https://doaj.org/article/fda80b945c5d4e9f819a3d620c27c1f92020-05-01T00:00:00Zhttps://jnephropharmacology.com/PDF/npj-2298https://doaj.org/toc/2345-4202Introduction: Reactive oxygen species (ROS) are the main factors in pathogenesis of renal ischemia-reperfusion (I/R) injury. Objectives: The aim of this study was to evaluate the renoprotective effect of rosmarinic acid (ROA) as an antioxidant substance against renal I/R injury. Materials and Methods: Forty male rats were divided into five groups. Group 1 control; group 2 ischemia reperfusion (I/R). Groups 3, 4 and 5 I/R treated by ROA 50, 100 and 200 mg/kg respectively. The treated groups (groups 3, 4 and 5) received ROA one hour before ischemia induction. Renal ischemia was induced by ligating of renal vessels through vascular clips. After 45 minutes of ischemia, the clips were removed to make renal recirculation (reperfusion). Twenty-four hours after the onset of reperfusion, under anesthesia blood were sampled and kidneys were removed. The serum and supernatant of renal homogenate were prepared. Serum creatinine, nitric oxide (NO) and paraoxonase (PON) were measured. The concentration of renal malondialdehyde (MDA), glutathione peroxidase (GPX), glutathione (GSH) and catalase (CAT) activity were assessed. Results: The administration of ROA, decreased serum creatinine and increased serum NO and PON compared to group 2 (P<0.05). Renal MDA, GSH, GPX and CAT activity improved significantly in animals that received ROA in comparison to group 2. Conclusion: Administration of ROA improved renal I/R injuries via inhibition of lipid peroxidation and increase of GSH, GPX, CAT, NO and PON.Majid TavafiHassan AhmadvandAhmad TamjidipourAfshin HasanvandSociety of Diabetic Nephropathy Prevention articleoxidative stresskidneyantioxidantischemia-reperfusionrosmarinic acidreactive oxygen speciesTherapeutics. PharmacologyRM1-950Diseases of the genitourinary system. UrologyRC870-923ENJournal of Nephropharmacology, Vol 9, Iss 2, Pp e15-e15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	oxidative stress kidney antioxidant ischemia-reperfusion rosmarinic acid reactive oxygen species Therapeutics. Pharmacology RM1-950 Diseases of the genitourinary system. Urology RC870-923
spellingShingle	oxidative stress kidney antioxidant ischemia-reperfusion rosmarinic acid reactive oxygen species Therapeutics. Pharmacology RM1-950 Diseases of the genitourinary system. Urology RC870-923 Majid Tavafi Hassan Ahmadvand Ahmad Tamjidipour Afshin Hasanvand Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats
description	Introduction: Reactive oxygen species (ROS) are the main factors in pathogenesis of renal ischemia-reperfusion (I/R) injury. Objectives: The aim of this study was to evaluate the renoprotective effect of rosmarinic acid (ROA) as an antioxidant substance against renal I/R injury. Materials and Methods: Forty male rats were divided into five groups. Group 1 control; group 2 ischemia reperfusion (I/R). Groups 3, 4 and 5 I/R treated by ROA 50, 100 and 200 mg/kg respectively. The treated groups (groups 3, 4 and 5) received ROA one hour before ischemia induction. Renal ischemia was induced by ligating of renal vessels through vascular clips. After 45 minutes of ischemia, the clips were removed to make renal recirculation (reperfusion). Twenty-four hours after the onset of reperfusion, under anesthesia blood were sampled and kidneys were removed. The serum and supernatant of renal homogenate were prepared. Serum creatinine, nitric oxide (NO) and paraoxonase (PON) were measured. The concentration of renal malondialdehyde (MDA), glutathione peroxidase (GPX), glutathione (GSH) and catalase (CAT) activity were assessed. Results: The administration of ROA, decreased serum creatinine and increased serum NO and PON compared to group 2 (P<0.05). Renal MDA, GSH, GPX and CAT activity improved significantly in animals that received ROA in comparison to group 2. Conclusion: Administration of ROA improved renal I/R injuries via inhibition of lipid peroxidation and increase of GSH, GPX, CAT, NO and PON.
format	article
author	Majid Tavafi Hassan Ahmadvand Ahmad Tamjidipour Afshin Hasanvand
author_facet	Majid Tavafi Hassan Ahmadvand Ahmad Tamjidipour Afshin Hasanvand
author_sort	Majid Tavafi
title	Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats
title_short	Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats
title_full	Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats
title_fullStr	Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats
title_full_unstemmed	Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats
title_sort	rosmarinic acid ameliorates renal ischemia reperfusion damage in rats
publisher	Society of Diabetic Nephropathy Prevention
publishDate	2020
url	https://doaj.org/article/fda80b945c5d4e9f819a3d620c27c1f9
work_keys_str_mv	AT majidtavafi rosmarinicacidamelioratesrenalischemiareperfusiondamageinrats AT hassanahmadvand rosmarinicacidamelioratesrenalischemiareperfusiondamageinrats AT ahmadtamjidipour rosmarinicacidamelioratesrenalischemiareperfusiondamageinrats AT afshinhasanvand rosmarinicacidamelioratesrenalischemiareperfusiondamageinrats
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Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats

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