Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters

Summary: Streptomyces species have attracted considerable interest as a reservoir of medically important secondary metabolites, which are even diverse and different between strains. Here, we reassess ten Streptomyces venezuelae strains by presenting the highly resolved classification, using 16S rRNA...

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Autores principales:	Namil Lee, Mira Choi, Woori Kim, Soonkyu Hwang, Yongjae Lee, Ji Hun Kim, Gahyeon Kim, Hyeseong Kim, Suhyung Cho, Sun Chang Kim, Bernhard Palsson, Kyoung-Soon Jang, Byung-Kwan Cho
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Microbiology Microbial genomics Phylogeny Metabolomics Science Q
Acceso en línea:	https://doaj.org/article/fdac5c047bd549ec9b9649a80924b464
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spelling	oai:doaj.org-article:fdac5c047bd549ec9b9649a80924b4642021-11-24T04:33:04ZRe-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters2589-004210.1016/j.isci.2021.103410https://doaj.org/article/fdac5c047bd549ec9b9649a80924b4642021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S258900422101381Xhttps://doaj.org/toc/2589-0042Summary: Streptomyces species have attracted considerable interest as a reservoir of medically important secondary metabolites, which are even diverse and different between strains. Here, we reassess ten Streptomyces venezuelae strains by presenting the highly resolved classification, using 16S rRNA sequencing, MALDI-TOF MS protein profiling, and whole-genome sequencing. The results revealed that seven of the ten strains were misclassified as S. venezuelae species. Secondary metabolite biosynthetic gene cluster (smBGC) mining and targeted LC-MS/MS based metabolite screening of S. venezuelae and misclassified strains identified in total 59 secondary metabolites production. In addition, a comparison of pyrrolamide-type antibiotic BGCs of four misclassified strains, followed by functional genomics, revealed that athv28 is critical in the synthesis of the anthelvencin precursor, 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate (ADPC). Our findings illustrate the importance of the accurate classification and better utilization of misclassified Streptomyces strains to discover smBGCs and their secondary metabolite products.Namil LeeMira ChoiWoori KimSoonkyu HwangYongjae LeeJi Hun KimGahyeon KimHyeseong KimSuhyung ChoSun Chang KimBernhard PalssonKyoung-Soon JangByung-Kwan ChoElsevierarticleMicrobiologyMicrobial genomicsPhylogenyMetabolomicsScienceQENiScience, Vol 24, Iss 12, Pp 103410- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology Microbial genomics Phylogeny Metabolomics Science Q
spellingShingle	Microbiology Microbial genomics Phylogeny Metabolomics Science Q Namil Lee Mira Choi Woori Kim Soonkyu Hwang Yongjae Lee Ji Hun Kim Gahyeon Kim Hyeseong Kim Suhyung Cho Sun Chang Kim Bernhard Palsson Kyoung-Soon Jang Byung-Kwan Cho Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters
description	Summary: Streptomyces species have attracted considerable interest as a reservoir of medically important secondary metabolites, which are even diverse and different between strains. Here, we reassess ten Streptomyces venezuelae strains by presenting the highly resolved classification, using 16S rRNA sequencing, MALDI-TOF MS protein profiling, and whole-genome sequencing. The results revealed that seven of the ten strains were misclassified as S. venezuelae species. Secondary metabolite biosynthetic gene cluster (smBGC) mining and targeted LC-MS/MS based metabolite screening of S. venezuelae and misclassified strains identified in total 59 secondary metabolites production. In addition, a comparison of pyrrolamide-type antibiotic BGCs of four misclassified strains, followed by functional genomics, revealed that athv28 is critical in the synthesis of the anthelvencin precursor, 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate (ADPC). Our findings illustrate the importance of the accurate classification and better utilization of misclassified Streptomyces strains to discover smBGCs and their secondary metabolite products.
format	article
author	Namil Lee Mira Choi Woori Kim Soonkyu Hwang Yongjae Lee Ji Hun Kim Gahyeon Kim Hyeseong Kim Suhyung Cho Sun Chang Kim Bernhard Palsson Kyoung-Soon Jang Byung-Kwan Cho
author_facet	Namil Lee Mira Choi Woori Kim Soonkyu Hwang Yongjae Lee Ji Hun Kim Gahyeon Kim Hyeseong Kim Suhyung Cho Sun Chang Kim Bernhard Palsson Kyoung-Soon Jang Byung-Kwan Cho
author_sort	Namil Lee
title	Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters
title_short	Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters
title_full	Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters
title_fullStr	Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters
title_full_unstemmed	Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters
title_sort	re-classification of streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/fdac5c047bd549ec9b9649a80924b464
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Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters

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