Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton

The coexistence of nanoplastics (NPs) and pollutants such as arsenic (As) has become an unignorable environmental problem. However, there is still a considerable knowledge gap about the impact of NPs and pollutants on human health risks. In this study, the human gastric adenocarcinoma (AGS) cells we...

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Autores principales:	Peichun Lin, Yitao Guo, Lei He, Xiuchun Liao, Xueru Chen, Liuying He, Zifan Lu, Zhong-Ji Qian, Chunxia Zhou, Pengzhi Hong, Shengli Sun, Chengyong Li
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Nanoplastics Arsenic AGS cells ABC transporter Cytoskeleton Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/fdb6e8574c7d4448af63f1158092a863
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spelling	oai:doaj.org-article:fdb6e8574c7d4448af63f1158092a8632021-11-06T04:14:42ZNanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton0147-651310.1016/j.ecoenv.2021.112885https://doaj.org/article/fdb6e8574c7d4448af63f1158092a8632021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321009970https://doaj.org/toc/0147-6513The coexistence of nanoplastics (NPs) and pollutants such as arsenic (As) has become an unignorable environmental problem. However, there is still a considerable knowledge gap about the impact of NPs and pollutants on human health risks. In this study, the human gastric adenocarcinoma (AGS) cells were used as a model to investigate the toxicity of NPs with different particle sizes and As by MTT assay, western blotting, immunofluorescence and so on. The results showed that 20 nm (8 μg/mL), 50 nm (128 μg/mL), 200 nm (128 μg/mL), 500 nm (128 μg/mL), 1000 nm (128 μg/mL) polystyrene (PS) did not affect cell viability, ROS, intracellular calcium and activate apoptosis pathway in AGS cells. However, noncytotoxic concentration of NPs enhanced the cytotoxicity and intracellular accumulation of As. NPs destroys the fluidity of cell membrane and cytoskeleton, inhibits the activity of ABC transporter, and leads to the accumulation of As in cells. This work highlights that the damage caused by NPs, especially at the level of noncytotoxicity, joint with As cannot be ignored and provides a specific toxicological mechanism of NPs accompanied by exposure to As.Peichun LinYitao GuoLei HeXiuchun LiaoXueru ChenLiuying HeZifan LuZhong-Ji QianChunxia ZhouPengzhi HongShengli SunChengyong LiElsevierarticleNanoplasticsArsenicAGS cellsABC transporterCytoskeletonEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112885- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanoplastics Arsenic AGS cells ABC transporter Cytoskeleton Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Nanoplastics Arsenic AGS cells ABC transporter Cytoskeleton Environmental pollution TD172-193.5 Environmental sciences GE1-350 Peichun Lin Yitao Guo Lei He Xiuchun Liao Xueru Chen Liuying He Zifan Lu Zhong-Ji Qian Chunxia Zhou Pengzhi Hong Shengli Sun Chengyong Li Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton
description	The coexistence of nanoplastics (NPs) and pollutants such as arsenic (As) has become an unignorable environmental problem. However, there is still a considerable knowledge gap about the impact of NPs and pollutants on human health risks. In this study, the human gastric adenocarcinoma (AGS) cells were used as a model to investigate the toxicity of NPs with different particle sizes and As by MTT assay, western blotting, immunofluorescence and so on. The results showed that 20 nm (8 μg/mL), 50 nm (128 μg/mL), 200 nm (128 μg/mL), 500 nm (128 μg/mL), 1000 nm (128 μg/mL) polystyrene (PS) did not affect cell viability, ROS, intracellular calcium and activate apoptosis pathway in AGS cells. However, noncytotoxic concentration of NPs enhanced the cytotoxicity and intracellular accumulation of As. NPs destroys the fluidity of cell membrane and cytoskeleton, inhibits the activity of ABC transporter, and leads to the accumulation of As in cells. This work highlights that the damage caused by NPs, especially at the level of noncytotoxicity, joint with As cannot be ignored and provides a specific toxicological mechanism of NPs accompanied by exposure to As.
format	article
author	Peichun Lin Yitao Guo Lei He Xiuchun Liao Xueru Chen Liuying He Zifan Lu Zhong-Ji Qian Chunxia Zhou Pengzhi Hong Shengli Sun Chengyong Li
author_facet	Peichun Lin Yitao Guo Lei He Xiuchun Liao Xueru Chen Liuying He Zifan Lu Zhong-Ji Qian Chunxia Zhou Pengzhi Hong Shengli Sun Chengyong Li
author_sort	Peichun Lin
title	Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton
title_short	Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton
title_full	Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton
title_fullStr	Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton
title_full_unstemmed	Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton
title_sort	nanoplastics aggravate the toxicity of arsenic to ags cells by disrupting abc transporter and cytoskeleton
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/fdb6e8574c7d4448af63f1158092a863
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Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton

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