hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.

Human Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulati...

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Autores principales:	Fen Li, Jixian Ma, Yu Ma, Yanyan Hu, Shujuan Tian, Richard E White, Guichun Han
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdb7a1b4e507423fa8b1536210d63601
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spelling	oai:doaj.org-article:fdb7a1b4e507423fa8b1536210d636012021-11-18T07:31:41ZhElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.1932-620310.1371/journal.pone.0029303https://doaj.org/article/fdb7a1b4e507423fa8b1536210d636012011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22216241/?tool=EBIhttps://doaj.org/toc/1932-6203Human Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulation of the human elongation protein 3 (hELP3) on the expression of HSP70 through antisense strategy. Transfecting antisense plasmid p1107 into HeLa cells highly suppressed hELP3 expression, and substantially reduced expression of HSP70 mRNA and protein. Furthermore, chromatin immunoprecipitation assay (ChIP Assay) revealed that hElp3 participates in the transcription elongation of HSPA1A in HeLa cells. Finally, complementation and ChIP Assay in yeast showed that hElp3 can not only complement the growth and slow activation of HSP70 (SSA3) gene transcription, but also directly regulates the transcription of SSA3. On the contrary, these functions are lost when the HAT domain is deleted from hElp3. These data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function. These findings now provide novel insights and evidence of the functions of hELP3 in human cells.Fen LiJixian MaYu MaYanyan HuShujuan TianRichard E WhiteGuichun HanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e29303 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fen Li Jixian Ma Yu Ma Yanyan Hu Shujuan Tian Richard E White Guichun Han hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.
description	Human Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulation of the human elongation protein 3 (hELP3) on the expression of HSP70 through antisense strategy. Transfecting antisense plasmid p1107 into HeLa cells highly suppressed hELP3 expression, and substantially reduced expression of HSP70 mRNA and protein. Furthermore, chromatin immunoprecipitation assay (ChIP Assay) revealed that hElp3 participates in the transcription elongation of HSPA1A in HeLa cells. Finally, complementation and ChIP Assay in yeast showed that hElp3 can not only complement the growth and slow activation of HSP70 (SSA3) gene transcription, but also directly regulates the transcription of SSA3. On the contrary, these functions are lost when the HAT domain is deleted from hElp3. These data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function. These findings now provide novel insights and evidence of the functions of hELP3 in human cells.
format	article
author	Fen Li Jixian Ma Yu Ma Yanyan Hu Shujuan Tian Richard E White Guichun Han
author_facet	Fen Li Jixian Ma Yu Ma Yanyan Hu Shujuan Tian Richard E White Guichun Han
author_sort	Fen Li
title	hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.
title_short	hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.
title_full	hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.
title_fullStr	hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.
title_full_unstemmed	hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.
title_sort	help3 directly modulates the expression of hsp70 gene in hela cells via hat activity.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fdb7a1b4e507423fa8b1536210d63601
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hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.

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