Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering

Abstract Inflammatory microenvironment causes the change of epigenetic modification in periodontal ligament stem cells derived from periodontitis tissues (P-PDLSCs), which results in defective osteogenic differentiation compared to cells from healthy tissues. It’s urgent to explore therapeutic strat...

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Autores principales:	Jin Sun, Zhiwei Dong, Yang Zhang, Xiaoning He, Dongdong Fei, Fang Jin, Lin Yuan, Bei Li, Yan Jin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdb9a53112ce4cb1a9b3078715e5cee8
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spelling	oai:doaj.org-article:fdb9a53112ce4cb1a9b3078715e5cee82021-12-02T16:07:01ZOsthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering10.1038/s41598-017-05762-72045-2322https://doaj.org/article/fdb9a53112ce4cb1a9b3078715e5cee82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05762-7https://doaj.org/toc/2045-2322Abstract Inflammatory microenvironment causes the change of epigenetic modification in periodontal ligament stem cells derived from periodontitis tissues (P-PDLSCs), which results in defective osteogenic differentiation compared to cells from healthy tissues. It’s urgent to explore therapeutic strategies aimed at epigenetic targets associated with the regenerative ability of PDLSCs. Osthole, a small-molecule compound extracted from Chinese herbs, has been documented to promote osteogenesis and cell sheets formation of healthy PDLSCs. However, whether osthole shows same effect on P-PDLSCs and the mechanism of promotive effect is still unknown. The purpose of this study was to determine whether Osthole could restore defective osteogenic differentiation of P-PDLSCs via epigenetic modification. We demonstrated that 10−7 Mol/L of Osthole was the best concentration for osteogenic differentiation and proliferation of P-PDLSCs. Mechanistically, we also found that Osthole upregulated MOZ and MORF, histone acetylases that specifically catalyze acetylation of Histone3 lisine9 (H3K9) and Histone3 lisine14 (H3K14), which are key regulators in osteogenic differentiation of P-PDLSCs. Furthermore, Osthole treatment improved cell sheet formation and enhanced the bone formation of PDLSC sheets in animal models of periodontitis. Our study suggests that Osthole is a promising drug to cure periodontitis via regulating epigenetic modification in cell sheets engineering.Jin SunZhiwei DongYang ZhangXiaoning HeDongdong FeiFang JinLin YuanBei LiYan JinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jin Sun Zhiwei Dong Yang Zhang Xiaoning He Dongdong Fei Fang Jin Lin Yuan Bei Li Yan Jin Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering
description	Abstract Inflammatory microenvironment causes the change of epigenetic modification in periodontal ligament stem cells derived from periodontitis tissues (P-PDLSCs), which results in defective osteogenic differentiation compared to cells from healthy tissues. It’s urgent to explore therapeutic strategies aimed at epigenetic targets associated with the regenerative ability of PDLSCs. Osthole, a small-molecule compound extracted from Chinese herbs, has been documented to promote osteogenesis and cell sheets formation of healthy PDLSCs. However, whether osthole shows same effect on P-PDLSCs and the mechanism of promotive effect is still unknown. The purpose of this study was to determine whether Osthole could restore defective osteogenic differentiation of P-PDLSCs via epigenetic modification. We demonstrated that 10−7 Mol/L of Osthole was the best concentration for osteogenic differentiation and proliferation of P-PDLSCs. Mechanistically, we also found that Osthole upregulated MOZ and MORF, histone acetylases that specifically catalyze acetylation of Histone3 lisine9 (H3K9) and Histone3 lisine14 (H3K14), which are key regulators in osteogenic differentiation of P-PDLSCs. Furthermore, Osthole treatment improved cell sheet formation and enhanced the bone formation of PDLSC sheets in animal models of periodontitis. Our study suggests that Osthole is a promising drug to cure periodontitis via regulating epigenetic modification in cell sheets engineering.
format	article
author	Jin Sun Zhiwei Dong Yang Zhang Xiaoning He Dongdong Fei Fang Jin Lin Yuan Bei Li Yan Jin
author_facet	Jin Sun Zhiwei Dong Yang Zhang Xiaoning He Dongdong Fei Fang Jin Lin Yuan Bei Li Yan Jin
author_sort	Jin Sun
title	Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering
title_short	Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering
title_full	Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering
title_fullStr	Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering
title_full_unstemmed	Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering
title_sort	osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fdb9a53112ce4cb1a9b3078715e5cee8
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Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering

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