Emerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand

Chytridiomycosis, a disease in amphibians caused by Batrachochytrium dendrobatidis (Bd), has led to a population decline and extinction of frog species since 1996. The objective of this study was to determine the prevalence of and the need for establishing a surveillance system for monitoring chytri...

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Autores principales:	Techangamsuwan Somporn, Sommanustweechai Angkana, Kamolnorranart Sumate, Siriaroonrat Boripat, Khonsue Wichase, Pirarat Nopadon
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	batrachochytrium dendrobatidis chytridiomycosis frog thailand Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/fdc294deb7e1455a9e03e5fd338b0e59
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spelling	oai:doaj.org-article:fdc294deb7e1455a9e03e5fd338b0e592021-11-17T21:27:51ZEmerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand1820-744810.1515/acve-2017-0042https://doaj.org/article/fdc294deb7e1455a9e03e5fd338b0e592017-12-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0042https://doaj.org/toc/1820-7448Chytridiomycosis, a disease in amphibians caused by Batrachochytrium dendrobatidis (Bd), has led to a population decline and extinction of frog species since 1996. The objective of this study was to determine the prevalence of and the need for establishing a surveillance system for monitoring chytridiomycosis in five national zoos and five free ranging protected areas across Thailand. A total of 492 skin swab samples were collected from live and dead animals and tested by polymerase chain reaction (PCR) for the presence of Bd. The positive specimens were confi rmed by amplicon sequencing and examined by histopathology and immunohistochemistry. From July 2009 to August 2012, the prevalence of Bd from frog skin samples was low (4.27%), monitored by PCR. All samples from live amphibians were negative. The positive cases were only from dead specimens (21/168, 12.5% dead samples) of two non-native captive species, poison dart frog (Dendrobates tinctorius) and tomato frog (Dyscophus antongilii) in one zoo. Immunohistochemistry and histopathology revealed the typical feature of fl ask-shaped zoosporangia and septate thalli, supporting the PCR-based evidence of chytridiomycosis in captive amphibians in Thailand, but detected Bd in only 7/21 of the PCR-positive samples. Although the introduction of a pathogenic strain of Bd from imported carriers might have a serious impact on the native amphibian populations in Thailand, chytridiomycosis has not currently been detected in native Thai amphibians. An active surveillance system is needed for close monitoring of the fungus crossing into Thai amphibian populationsTechangamsuwan SompornSommanustweechai AngkanaKamolnorranart SumateSiriaroonrat BoripatKhonsue WichasePirarat NopadonSciendoarticlebatrachochytrium dendrobatidischytridiomycosisfrogthailandVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 4, Pp 525-539 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	batrachochytrium dendrobatidis chytridiomycosis frog thailand Veterinary medicine SF600-1100
spellingShingle	batrachochytrium dendrobatidis chytridiomycosis frog thailand Veterinary medicine SF600-1100 Techangamsuwan Somporn Sommanustweechai Angkana Kamolnorranart Sumate Siriaroonrat Boripat Khonsue Wichase Pirarat Nopadon Emerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand
description	Chytridiomycosis, a disease in amphibians caused by Batrachochytrium dendrobatidis (Bd), has led to a population decline and extinction of frog species since 1996. The objective of this study was to determine the prevalence of and the need for establishing a surveillance system for monitoring chytridiomycosis in five national zoos and five free ranging protected areas across Thailand. A total of 492 skin swab samples were collected from live and dead animals and tested by polymerase chain reaction (PCR) for the presence of Bd. The positive specimens were confi rmed by amplicon sequencing and examined by histopathology and immunohistochemistry. From July 2009 to August 2012, the prevalence of Bd from frog skin samples was low (4.27%), monitored by PCR. All samples from live amphibians were negative. The positive cases were only from dead specimens (21/168, 12.5% dead samples) of two non-native captive species, poison dart frog (Dendrobates tinctorius) and tomato frog (Dyscophus antongilii) in one zoo. Immunohistochemistry and histopathology revealed the typical feature of fl ask-shaped zoosporangia and septate thalli, supporting the PCR-based evidence of chytridiomycosis in captive amphibians in Thailand, but detected Bd in only 7/21 of the PCR-positive samples. Although the introduction of a pathogenic strain of Bd from imported carriers might have a serious impact on the native amphibian populations in Thailand, chytridiomycosis has not currently been detected in native Thai amphibians. An active surveillance system is needed for close monitoring of the fungus crossing into Thai amphibian populations
format	article
author	Techangamsuwan Somporn Sommanustweechai Angkana Kamolnorranart Sumate Siriaroonrat Boripat Khonsue Wichase Pirarat Nopadon
author_facet	Techangamsuwan Somporn Sommanustweechai Angkana Kamolnorranart Sumate Siriaroonrat Boripat Khonsue Wichase Pirarat Nopadon
author_sort	Techangamsuwan Somporn
title	Emerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand
title_short	Emerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand
title_full	Emerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand
title_fullStr	Emerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand
title_full_unstemmed	Emerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand
title_sort	emerging chytrid fungal pathogen, batrachochytrium dendrobatidis, in zoo amphibians in thailand
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/fdc294deb7e1455a9e03e5fd338b0e59
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Emerging Chytrid Fungal Pathogen, Batrachochytrium Dendrobatidis, in Zoo Amphibians in Thailand

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