Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.

<h4>Background</h4>Endomyocardial Fibrosis (EMF) is a tropical restrictive cardiomyopathy of unknown etiology with high prevalence in Sub-Saharan Africa, for which it is unclear whether the primary target of injury is the endocardial endothelium, the subendocardial fibroblast, the corona...

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Autores principales:	Ana Olga Mocumbi, Najma Latif, Magdi H Yacoub
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/fdc723fb100f4affa50a319c0a936e36
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spelling	oai:doaj.org-article:fdc723fb100f4affa50a319c0a936e362021-11-25T06:33:35ZPresence of circulating anti-myosin antibodies in endomyocardial fibrosis.1935-27271935-273510.1371/journal.pntd.0000661https://doaj.org/article/fdc723fb100f4affa50a319c0a936e362010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20422043/pdf/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Endomyocardial Fibrosis (EMF) is a tropical restrictive cardiomyopathy of unknown etiology with high prevalence in Sub-Saharan Africa, for which it is unclear whether the primary target of injury is the endocardial endothelium, the subendocardial fibroblast, the coronary microcirculation or the myocyte. In an attempt to explore the possibility of endocardial lesions being a result of an immune response against the myocyte we assessed the presence and frequency of circulating anti-myocardial antibodies in EMF patients.<h4>Methodology/principal findings</h4>EMF classification, assessment of severity and staging was based on echocardiography. We used sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) of myocardial proteins followed by western blotting to screen serum samples for antiheart antibodies G and M classes. The degree of serum reactivity was correlated with the severity and activity of EMF. We studied 56 EMF patients and 10 healthy controls. IgG reactivity against myocardial proteins was stronger and more frequent in patients with EMF when compared to controls (30/56; 53.6% vs. 1/10; 10%, respectively). IgM reactivity was weak in both groups, although higher in EMF patients (11/56; 19.6%) when compared to controls (n = 0). EMF patients showed greater frequency and reactivity of IgG antibodies against myocardial proteins of molecular weights 35 kD, 42 kD and 70 kD (p values <0.01, <0.01 and <0.05 respectively).<h4>Conclusions</h4>The presence of antibodies against myocardial proteins was demonstrated in a subset of EMF patients. These immune markers seem to be related with activity and might provide an adjunct tool for diagnosis and classification of EMF, therefore improving its management by identifying patients who may benefit from immunosuppressive therapy. Further research is needed to clarify the role of autoimmunity in the pathogenesis of EMF.Ana Olga MocumbiNajma LatifMagdi H YacoubPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 4, Iss 4, p e661 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ana Olga Mocumbi Najma Latif Magdi H Yacoub Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.
description	<h4>Background</h4>Endomyocardial Fibrosis (EMF) is a tropical restrictive cardiomyopathy of unknown etiology with high prevalence in Sub-Saharan Africa, for which it is unclear whether the primary target of injury is the endocardial endothelium, the subendocardial fibroblast, the coronary microcirculation or the myocyte. In an attempt to explore the possibility of endocardial lesions being a result of an immune response against the myocyte we assessed the presence and frequency of circulating anti-myocardial antibodies in EMF patients.<h4>Methodology/principal findings</h4>EMF classification, assessment of severity and staging was based on echocardiography. We used sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) of myocardial proteins followed by western blotting to screen serum samples for antiheart antibodies G and M classes. The degree of serum reactivity was correlated with the severity and activity of EMF. We studied 56 EMF patients and 10 healthy controls. IgG reactivity against myocardial proteins was stronger and more frequent in patients with EMF when compared to controls (30/56; 53.6% vs. 1/10; 10%, respectively). IgM reactivity was weak in both groups, although higher in EMF patients (11/56; 19.6%) when compared to controls (n = 0). EMF patients showed greater frequency and reactivity of IgG antibodies against myocardial proteins of molecular weights 35 kD, 42 kD and 70 kD (p values <0.01, <0.01 and <0.05 respectively).<h4>Conclusions</h4>The presence of antibodies against myocardial proteins was demonstrated in a subset of EMF patients. These immune markers seem to be related with activity and might provide an adjunct tool for diagnosis and classification of EMF, therefore improving its management by identifying patients who may benefit from immunosuppressive therapy. Further research is needed to clarify the role of autoimmunity in the pathogenesis of EMF.
format	article
author	Ana Olga Mocumbi Najma Latif Magdi H Yacoub
author_facet	Ana Olga Mocumbi Najma Latif Magdi H Yacoub
author_sort	Ana Olga Mocumbi
title	Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.
title_short	Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.
title_full	Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.
title_fullStr	Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.
title_full_unstemmed	Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.
title_sort	presence of circulating anti-myosin antibodies in endomyocardial fibrosis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/fdc723fb100f4affa50a319c0a936e36
work_keys_str_mv	AT anaolgamocumbi presenceofcirculatingantimyosinantibodiesinendomyocardialfibrosis AT najmalatif presenceofcirculatingantimyosinantibodiesinendomyocardialfibrosis AT magdihyacoub presenceofcirculatingantimyosinantibodiesinendomyocardialfibrosis
_version_	1718413658480967680

Presence of circulating anti-myosin antibodies in endomyocardial fibrosis.

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