A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for <i>In Vitro</i> Studies in AMD

Código QR

A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for <i>In Vitro</i> Studies in AMD

Degeneration of retinal pigment epithelium (RPE) is one of the most critical phenotypic changes of age-related macular degeneration (AMD), the leading cause of vision loss in the elderly. While cultured polarized RPE cells with original properties are valuable in <i>in vitro</i> models t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Peng Shang, Nadezda A. Stepicheva, Haitao Liu, Olivia Chowdhury, Jonathan Franks, Ming Sun, Stacey Hose, Sayan Ghosh, Meysam Yazdankhah, Anastasia Strizhakova, Donna Beer Stolz, J. Samuel Zigler, Debasish Sinha
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	retina pigment epithelium explant culture RPE flatmount adenoviral transduction Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fdcc07a5c43e4d50967ebb8968f7dac1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares

Complement Factor H Loss in RPE Cells Causes Retinal Degeneration in a Human RPE-Porcine Retinal Explant Co-Culture Model
por: Angela Armento, et al.
Publicado: (2021)

Examining the Role of Cone-expressed RPE65 in Mouse Cone Function
por: Alexander V. Kolesnikov, et al.
Publicado: (2018)

Management of Adenoviral Keratoconjunctivitis: Challenges and Solutions
por: Labib BA, et al.
Publicado: (2020)

Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
por: Mahesh,Ayyavu, et al.
Publicado: (2012)

Clinical Features and Natural History in a Cohort of Chinese Patients with RPE65-Associated Inherited Retinal Dystrophy
por: Jie Shi, et al.
Publicado: (2021)

Influence of Growth Regulators and Explant on Plant Regeneration in Tomato
por: Atul Batra, et al.
Publicado: (2011)

Long-Term Transplant Effects of iPSC-RPE Monolayer in Immunodeficient RCS Rats
por: Deepthi S. Rajendran Nair, et al.
Publicado: (2021)

An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb
por: Pari DEHVARI-NAGAN, et al.
Publicado: (2021)

DEVELOPMENT OF VACCINES BASED ON ADENOVIRAL VECTORS: A REVIEW OF FOREIGN CLINICAL STUDIES (PART 1)
por: L. V. Cherenova, et al.
Publicado: (2017)

DEVELOPMENT OF VACCINES BASED ON ADENOVIRAL VECTORS: A REVIEW OF FOREIGN CLINICAL STUDIES (PART 2)
por: L. V. Cherenova, et al.
Publicado: (2017)

Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant
por: D P Prakash, et al.
Publicado: (2012)

Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
por: D P Prakash, et al.
Publicado: (2007)

Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata
por: Mehta Nikhil, et al.
Publicado: (2021)

Research progress on the mechanism of epithelial mesenchymal transformation in RPE cells and the treatment of PVR
por: Hai-Yue Cui, et al.
Publicado: (2021)

La sobreexpresión del gen de enzima convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
por: Ramos,Elizabeth, et al.
Publicado: (2010)

RETRACTED ARTICLE: Non-coding RNAome of RPE cells under oxidative stress suggests unknown regulative aspects of Retinitis pigmentosa etiopathogenesis
por: Luigi Donato, et al.
Publicado: (2018)

Clinical Presentations and Comparative Outcomes of Delayed-Onset Low-Grade Endophthalmitis Managed with or Without Intraocular Lens Explantation
por: Dave VP, et al.
Publicado: (2020)

Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl
por: Shabeer Ahmad Dar, et al.
Publicado: (2021)

Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods
por: Erma Prihastanti, et al.
Publicado: (2020)

A randomized controlled trial of povidone-iodine/dexamethasone ophthalmic suspension for acute viral conjunctivitis
por: Pepose JS, et al.
Publicado: (2019)

Scaffold-Free Retinal Pigment Epithelium Microtissues Exhibit Increased Release of PEDF
por: Abdullah Al-Ani, et al.
Publicado: (2021)

Reference Values for External and Internal Training Intensity Monitoring in Young Male Soccer Players: A Systematic Review
por: Rafael Oliveira, et al.
Publicado: (2021)

Chemical Components and Biological Activities of Essential Oils of <i>Mentha × piperita</i> L. from Field-Grown and Field-Acclimated after In Vitro Propagation Plants
por: Olga V. Shelepova, et al.
Publicado: (2021)

Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots
por: Ríos-Ramírez,Suzel C., et al.
Publicado: (2017)

Fluctuations of Training Load Variables in Elite Soccer Players U-14 throughout the Competition Season
por: Hadi Nobari, et al.
Publicado: (2021)

A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees
por: Prakash Patil, et al.
Publicado: (2007)

Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters
por: Jakob Trimpert, et al.
Publicado: (2021)

Influence of Congested Match Schedules, Pre-Match Well-Being and Level of Opponents on Match Loads during World Rugby Women’s Sevens Series
por: Daniele Conte, et al.
Publicado: (2021)

A Virtual Reality Orientation and Mobility Test for Inherited Retinal Degenerations: Testing a Proof-of-Concept After Gene Therapy
por: Aleman TS, et al.
Publicado: (2021)

Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)
por: Koushik Dutta, et al.
Publicado: (2016)

Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation
por: D P Prakash, et al.
Publicado: (2015)

βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells
por: Peng Shang, et al.
Publicado: (2021)

Removing Well-Fixed, Collared and Noncollared Tapered Hip Stems Without an Extended Trochanteric Osteotomy Using a Novel Stem Removal System
por: Kevin M. Sagers, DO, et al.
Publicado: (2021)

Osmolarity and spectrophotometric property of brilliant blue green define the degree of toxicity on retinal pigment epithelial cells exposed to surgical endoilluminator
por: Balaiya S, et al.
Publicado: (2016)

Evaluation of an oral telomerase activator for early age-related macular degeneration - a pilot study
por: Dow CT, et al.
Publicado: (2016)

Multiocular organoids from human induced pluripotent stem cells displayed retinal, corneal, and retinal pigment epithelium lineages
por: Helena Isla-Magrané, et al.
Publicado: (2021)

Voretigene Neparvovec in Retinal Diseases: A Review of the Current Clinical Evidence
por: Gao J, et al.
Publicado: (2020)

Algorithms for optoacoustically controlled selective retina therapy (SRT)
por: Eric Seifert, et al.
Publicado: (2022)

Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
por: Takeyama,Masayuki, et al.
Publicado: (2015)

An approach to grossing of hepatectomy specimens
por: Archana Rastogi
Publicado: (2021)