A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for <i>In Vitro</i> Studies in AMD

A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for <i>In Vitro</i> Studies in AMD

Degeneration of retinal pigment epithelium (RPE) is one of the most critical phenotypic changes of age-related macular degeneration (AMD), the leading cause of vision loss in the elderly. While cultured polarized RPE cells with original properties are valuable in <i>in vitro</i> models t...

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Bibliographic Details
Main Authors: Peng Shang, Nadezda A. Stepicheva, Haitao Liu, Olivia Chowdhury, Jonathan Franks, Ming Sun, Stacey Hose, Sayan Ghosh, Meysam Yazdankhah, Anastasia Strizhakova, Donna Beer Stolz, J. Samuel Zigler, Debasish Sinha
Format: article
Language:EN
Published: MDPI AG 2021
Subjects:
retina pigment epithelium
explant culture
RPE flatmount
adenoviral transduction
Biology (General)
QH301-705.5
Chemistry
QD1-999
Online Access:https://doaj.org/article/fdcc07a5c43e4d50967ebb8968f7dac1
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