Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.

H2A.Z is a histone H2A variant conserved from yeast to humans, and is found at 63% of promoters in Saccharomyces cerevisiae. This pattern of localization suggests that H2A.Z is somehow important for gene expression or regulation. H2A.Z can be acetylated at up to four lysine residues on its amino-ter...

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Autores principales:	Jeffrey E Halley, Tommy Kaplan, Alice Y Wang, Michael S Kobor, Jasper Rine
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fdce0f5c0e75401db5ae279cf718bd03
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spelling	oai:doaj.org-article:fdce0f5c0e75401db5ae279cf718bd032021-12-02T19:54:48ZRoles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.1544-91731545-788510.1371/journal.pbio.1000401https://doaj.org/article/fdce0f5c0e75401db5ae279cf718bd032010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20582323/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885H2A.Z is a histone H2A variant conserved from yeast to humans, and is found at 63% of promoters in Saccharomyces cerevisiae. This pattern of localization suggests that H2A.Z is somehow important for gene expression or regulation. H2A.Z can be acetylated at up to four lysine residues on its amino-terminal tail, and acetylated-H2A.Z is enriched in chromatin containing promoters of active genes. We investigated whether H2A.Z's role in GAL1 gene regulation and gene expression depends on H2A.Z acetylation. Our findings suggested that H2A.Z functioned both in gene regulation and in gene expression and that only its role in gene regulation depended upon its acetylation. Our findings provided an alternate explanation for results that were previously interpreted as evidence that H2A.Z plays a role in GAL1 transcriptional memory. Additionally, our findings provided new insights into the phenotypes of htz1Delta mutants: in the absence of H2A.Z, the SWR1 complex, which deposits H2A.Z into chromatin, was deleterious to the cell, and many of the phenotypes of cells lacking H2A.Z were due to the SWR1 complex's activity rather than to the absence of H2A.Z per se. These results highlight the need to reevaluate all studies on the phenotypes of cells lacking H2A.Z.Jeffrey E HalleyTommy KaplanAlice Y WangMichael S KoborJasper RinePublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 8, Iss 6, p e1000401 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jeffrey E Halley Tommy Kaplan Alice Y Wang Michael S Kobor Jasper Rine Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.
description	H2A.Z is a histone H2A variant conserved from yeast to humans, and is found at 63% of promoters in Saccharomyces cerevisiae. This pattern of localization suggests that H2A.Z is somehow important for gene expression or regulation. H2A.Z can be acetylated at up to four lysine residues on its amino-terminal tail, and acetylated-H2A.Z is enriched in chromatin containing promoters of active genes. We investigated whether H2A.Z's role in GAL1 gene regulation and gene expression depends on H2A.Z acetylation. Our findings suggested that H2A.Z functioned both in gene regulation and in gene expression and that only its role in gene regulation depended upon its acetylation. Our findings provided an alternate explanation for results that were previously interpreted as evidence that H2A.Z plays a role in GAL1 transcriptional memory. Additionally, our findings provided new insights into the phenotypes of htz1Delta mutants: in the absence of H2A.Z, the SWR1 complex, which deposits H2A.Z into chromatin, was deleterious to the cell, and many of the phenotypes of cells lacking H2A.Z were due to the SWR1 complex's activity rather than to the absence of H2A.Z per se. These results highlight the need to reevaluate all studies on the phenotypes of cells lacking H2A.Z.
format	article
author	Jeffrey E Halley Tommy Kaplan Alice Y Wang Michael S Kobor Jasper Rine
author_facet	Jeffrey E Halley Tommy Kaplan Alice Y Wang Michael S Kobor Jasper Rine
author_sort	Jeffrey E Halley
title	Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.
title_short	Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.
title_full	Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.
title_fullStr	Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.
title_full_unstemmed	Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.
title_sort	roles for h2a.z and its acetylation in gal1 transcription and gene induction, but not gal1-transcriptional memory.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/fdce0f5c0e75401db5ae279cf718bd03
work_keys_str_mv	AT jeffreyehalley rolesforh2azanditsacetylationingal1transcriptionandgeneinductionbutnotgal1transcriptionalmemory AT tommykaplan rolesforh2azanditsacetylationingal1transcriptionandgeneinductionbutnotgal1transcriptionalmemory AT aliceywang rolesforh2azanditsacetylationingal1transcriptionandgeneinductionbutnotgal1transcriptionalmemory AT michaelskobor rolesforh2azanditsacetylationingal1transcriptionandgeneinductionbutnotgal1transcriptionalmemory AT jasperrine rolesforh2azanditsacetylationingal1transcriptionandgeneinductionbutnotgal1transcriptionalmemory
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Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memory.

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