Cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).

Diacylglycerol acyltransferase (DGAT) catalyzes the final and only committed acylation step in the synthesis of triacylglycerols. In this study, three novel AhDGATs genes were identified and isolated from peanut. Quantitative real-time RT-PCR analysis indicated that the AhDGAT1-2 transcript was more...

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Autores principales:	Xiaoyuan Chi, Ruibo Hu, Xiaowen Zhang, Mingna Chen, Na Chen, Lijuan Pan, Tong Wang, Mian Wang, Zhen Yang, Quanfu Wang, Shanlin Yu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdd672ed49824b8c8e0a5e7714355d92
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spelling	oai:doaj.org-article:fdd672ed49824b8c8e0a5e7714355d922021-11-25T06:02:12ZCloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).1932-620310.1371/journal.pone.0105834https://doaj.org/article/fdd672ed49824b8c8e0a5e7714355d922014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25181516/?tool=EBIhttps://doaj.org/toc/1932-6203Diacylglycerol acyltransferase (DGAT) catalyzes the final and only committed acylation step in the synthesis of triacylglycerols. In this study, three novel AhDGATs genes were identified and isolated from peanut. Quantitative real-time RT-PCR analysis indicated that the AhDGAT1-2 transcript was more abundant in roots, seeds, and cotyledons, whereas the transcript abundances of AhDGAT1-1 and AhDGAT3-3 were higher in flowers than in the other tissues examined. During seed development, transcript levels of AhDGAT1-1 remained relatively low during the initial developmental stage but increased gradually during later stages, peaking at 50 days after pegging (DAP). Levels of AhDGAT1-2 transcripts were higher at 10 and 60 DAPs and much lower during other stages, whereas AhDGAT3-3 showed higher expression levels at 20 and 50 DAPs. In addition, AhDGAT transcripts were differentially expressed following exposure to abiotic stresses or abscisic acid. The activity of the three AhDGAT genes was confirmed by heterologous expression in a Saccharomyces cerevisiae TAG-deficient quadruple mutant. The recombinant yeasts restored lipid body formation and TAG biosynthesis, and preferentially incorporated unsaturated C18 fatty acids into lipids. The present study provides significant information useful in modifying the oil deposition of peanut through molecular breeding.Xiaoyuan ChiRuibo HuXiaowen ZhangMingna ChenNa ChenLijuan PanTong WangMian WangZhen YangQuanfu WangShanlin YuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e105834 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoyuan Chi Ruibo Hu Xiaowen Zhang Mingna Chen Na Chen Lijuan Pan Tong Wang Mian Wang Zhen Yang Quanfu Wang Shanlin Yu Cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).
description	Diacylglycerol acyltransferase (DGAT) catalyzes the final and only committed acylation step in the synthesis of triacylglycerols. In this study, three novel AhDGATs genes were identified and isolated from peanut. Quantitative real-time RT-PCR analysis indicated that the AhDGAT1-2 transcript was more abundant in roots, seeds, and cotyledons, whereas the transcript abundances of AhDGAT1-1 and AhDGAT3-3 were higher in flowers than in the other tissues examined. During seed development, transcript levels of AhDGAT1-1 remained relatively low during the initial developmental stage but increased gradually during later stages, peaking at 50 days after pegging (DAP). Levels of AhDGAT1-2 transcripts were higher at 10 and 60 DAPs and much lower during other stages, whereas AhDGAT3-3 showed higher expression levels at 20 and 50 DAPs. In addition, AhDGAT transcripts were differentially expressed following exposure to abiotic stresses or abscisic acid. The activity of the three AhDGAT genes was confirmed by heterologous expression in a Saccharomyces cerevisiae TAG-deficient quadruple mutant. The recombinant yeasts restored lipid body formation and TAG biosynthesis, and preferentially incorporated unsaturated C18 fatty acids into lipids. The present study provides significant information useful in modifying the oil deposition of peanut through molecular breeding.
format	article
author	Xiaoyuan Chi Ruibo Hu Xiaowen Zhang Mingna Chen Na Chen Lijuan Pan Tong Wang Mian Wang Zhen Yang Quanfu Wang Shanlin Yu
author_facet	Xiaoyuan Chi Ruibo Hu Xiaowen Zhang Mingna Chen Na Chen Lijuan Pan Tong Wang Mian Wang Zhen Yang Quanfu Wang Shanlin Yu
author_sort	Xiaoyuan Chi
title	Cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).
title_short	Cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).
title_full	Cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).
title_fullStr	Cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).
title_full_unstemmed	Cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).
title_sort	cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (arachis hypogaea l.).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fdd672ed49824b8c8e0a5e7714355d92
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Cloning and functional analysis of three diacylglycerol acyltransferase genes from peanut (Arachis hypogaea L.).

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