Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa

Background and Aim: Infections with Campylobacter species have gained recognition as the most frequent cause of foodborne gastroenteritis globally. Their significance in South Africa is still an area of study interest. This study was, therefore, carried out to determine the occurrence of Campylobact...

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Autores principales:	Kealeboga Mileng, Tsepo A. Ramatla, Rendani V. Ndou, Oriel M. M. Thekisoe, Michelo Syakalima
Formato:	article
Lenguaje:	EN
Publicado:	Veterinary World 2021
Materias:	antibiotic resistance campylobacter jejuni chickens south africa Animal culture SF1-1100 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/fdd718166d684c2eabd1352b012d873c
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spelling	oai:doaj.org-article:fdd718166d684c2eabd1352b012d873c2021-11-21T15:07:11ZIsolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa10.14202/vetworld.2021.2929-29350972-89882231-0916https://doaj.org/article/fdd718166d684c2eabd1352b012d873c2021-11-01T00:00:00Zhttp://www.veterinaryworld.org/Vol.14/November-2021/12.pdfhttps://doaj.org/toc/0972-8988https://doaj.org/toc/2231-0916Background and Aim: Infections with Campylobacter species have gained recognition as the most frequent cause of foodborne gastroenteritis globally. Their significance in South Africa is still an area of study interest. This study was, therefore, carried out to determine the occurrence of Campylobacter species in chickens from North West Province of South Africa as well as their antibiotic sensitivity status. Materials and Methods: A total of 2400 chicken fecal samples were collected and pooled to a total of 480 samples from five registered active poultry abattoirs in the Ngaka Modiri Molema District of North West Province, South Africa. Polymerase chain reaction (PCR) was used for the detection of Campylobacter spp. targeting the 16S rRNA gene while antibiotic sensitivity was determined using disk diffusion inhibition test. Results: After isolation, a total of 26 samples were confirmed to be harboring Campylobacter jejuni by PCR and sequencing. C. jejuni was found to be the only isolate detected in all the fecal samples tested. The study further demonstrated that C. jejuni infections were highest in the summer season (3%) followed by autumn and winter at 1%, while there were none detected in the spring. The isolated C. jejuni-positive samples on disk diffusion inhibition test displayed resistance to nalidixic acid, tetracycline, erythromycin, and ciprofloxacin at 98%, 80%, 83%, and 21%, respectively. Conclusion: C. jejuni isolated in this study is known to cause disease in humans, and thus its occurrence requires application of "One Health" strategy to reduce the spread of this zoonotic pathogen in South Africa.Kealeboga MilengTsepo A. RamatlaRendani V. NdouOriel M. M. ThekisoeMichelo SyakalimaVeterinary Worldarticleantibiotic resistancecampylobacter jejunichickenssouth africaAnimal cultureSF1-1100Veterinary medicineSF600-1100ENVeterinary World, Vol 14, Iss 11, Pp 2929-2935 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance campylobacter jejuni chickens south africa Animal culture SF1-1100 Veterinary medicine SF600-1100
spellingShingle	antibiotic resistance campylobacter jejuni chickens south africa Animal culture SF1-1100 Veterinary medicine SF600-1100 Kealeboga Mileng Tsepo A. Ramatla Rendani V. Ndou Oriel M. M. Thekisoe Michelo Syakalima Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa
description	Background and Aim: Infections with Campylobacter species have gained recognition as the most frequent cause of foodborne gastroenteritis globally. Their significance in South Africa is still an area of study interest. This study was, therefore, carried out to determine the occurrence of Campylobacter species in chickens from North West Province of South Africa as well as their antibiotic sensitivity status. Materials and Methods: A total of 2400 chicken fecal samples were collected and pooled to a total of 480 samples from five registered active poultry abattoirs in the Ngaka Modiri Molema District of North West Province, South Africa. Polymerase chain reaction (PCR) was used for the detection of Campylobacter spp. targeting the 16S rRNA gene while antibiotic sensitivity was determined using disk diffusion inhibition test. Results: After isolation, a total of 26 samples were confirmed to be harboring Campylobacter jejuni by PCR and sequencing. C. jejuni was found to be the only isolate detected in all the fecal samples tested. The study further demonstrated that C. jejuni infections were highest in the summer season (3%) followed by autumn and winter at 1%, while there were none detected in the spring. The isolated C. jejuni-positive samples on disk diffusion inhibition test displayed resistance to nalidixic acid, tetracycline, erythromycin, and ciprofloxacin at 98%, 80%, 83%, and 21%, respectively. Conclusion: C. jejuni isolated in this study is known to cause disease in humans, and thus its occurrence requires application of "One Health" strategy to reduce the spread of this zoonotic pathogen in South Africa.
format	article
author	Kealeboga Mileng Tsepo A. Ramatla Rendani V. Ndou Oriel M. M. Thekisoe Michelo Syakalima
author_facet	Kealeboga Mileng Tsepo A. Ramatla Rendani V. Ndou Oriel M. M. Thekisoe Michelo Syakalima
author_sort	Kealeboga Mileng
title	Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa
title_short	Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa
title_full	Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa
title_fullStr	Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa
title_full_unstemmed	Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa
title_sort	isolation and antibiotic sensitivity of campylobacter species from fecal samples of broiler chickens in north west province, south africa
publisher	Veterinary World
publishDate	2021
url	https://doaj.org/article/fdd718166d684c2eabd1352b012d873c
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Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa

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