Transcriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells

Abstract For many years, human peripheral blood natural killer (NK) cells have been divided into functionally distinct CD3− CD56bright CD16− and CD3− CD56dim CD16+ subsets. Recently, several groups of innate lymphoid cells (ILC), distinct from NK cells in development and function, have been defined...

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Autores principales:	David S. J. Allan, Ana Sofia Cerdeira, Anuisa Ranjan, Christina L. Kirkham, Oscar A. Aguilar, Miho Tanaka, Richard W. Childs, Cynthia E. Dunbar, Jack L. Strominger, Hernan D. Kopcow, James R. Carlyle
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdd8503caef444f796bf5bf324863b3b
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spelling	oai:doaj.org-article:fdd8503caef444f796bf5bf324863b3b2021-12-02T15:05:12ZTranscriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells10.1038/s41598-017-03256-02045-2322https://doaj.org/article/fdd8503caef444f796bf5bf324863b3b2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03256-0https://doaj.org/toc/2045-2322Abstract For many years, human peripheral blood natural killer (NK) cells have been divided into functionally distinct CD3− CD56bright CD16− and CD3− CD56dim CD16+ subsets. Recently, several groups of innate lymphoid cells (ILC), distinct from NK cells in development and function, have been defined in mouse. A signature of genes present in mouse ILC except NK cells, defined by Immunological Genome Project studies, is significantly over-represented in human CD56bright cells, by gene set enrichment analysis. Conversely, the signature genes of mouse NK cells are enriched in human CD56dim cells. Correlations are based upon large differences in expression of a few key genes. CD56bright cells show preferential expression of ILC-associated IL7R (CD127), TNFSF10 (TRAIL), KIT (CD117), IL2RA (CD25), CD27, CXCR3, DPP4 (CD26), GPR183, and MHC class II transcripts and proteins. This could indicate an ontological relationship between human CD56bright cells and mouse CD127+ ILC, or conserved networks of transcriptional regulation. In line with the latter hypothesis, among transcription factors known to impact ILC or NK cell development, GATA3, TCF7 (TCF-1), AHR, SOX4, RUNX2, and ZEB1 transcript levels are higher in CD56bright cells, while IKZF3 (AIOLOS), TBX21 (T-bet), NFIL3 (E4BP4), ZEB2, PRDM1 (BLIMP1), and RORA mRNA levels are higher in CD56dim cells.David S. J. AllanAna Sofia CerdeiraAnuisa RanjanChristina L. KirkhamOscar A. AguilarMiho TanakaRichard W. ChildsCynthia E. DunbarJack L. StromingerHernan D. KopcowJames R. CarlyleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David S. J. Allan Ana Sofia Cerdeira Anuisa Ranjan Christina L. Kirkham Oscar A. Aguilar Miho Tanaka Richard W. Childs Cynthia E. Dunbar Jack L. Strominger Hernan D. Kopcow James R. Carlyle Transcriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells
description	Abstract For many years, human peripheral blood natural killer (NK) cells have been divided into functionally distinct CD3− CD56bright CD16− and CD3− CD56dim CD16+ subsets. Recently, several groups of innate lymphoid cells (ILC), distinct from NK cells in development and function, have been defined in mouse. A signature of genes present in mouse ILC except NK cells, defined by Immunological Genome Project studies, is significantly over-represented in human CD56bright cells, by gene set enrichment analysis. Conversely, the signature genes of mouse NK cells are enriched in human CD56dim cells. Correlations are based upon large differences in expression of a few key genes. CD56bright cells show preferential expression of ILC-associated IL7R (CD127), TNFSF10 (TRAIL), KIT (CD117), IL2RA (CD25), CD27, CXCR3, DPP4 (CD26), GPR183, and MHC class II transcripts and proteins. This could indicate an ontological relationship between human CD56bright cells and mouse CD127+ ILC, or conserved networks of transcriptional regulation. In line with the latter hypothesis, among transcription factors known to impact ILC or NK cell development, GATA3, TCF7 (TCF-1), AHR, SOX4, RUNX2, and ZEB1 transcript levels are higher in CD56bright cells, while IKZF3 (AIOLOS), TBX21 (T-bet), NFIL3 (E4BP4), ZEB2, PRDM1 (BLIMP1), and RORA mRNA levels are higher in CD56dim cells.
format	article
author	David S. J. Allan Ana Sofia Cerdeira Anuisa Ranjan Christina L. Kirkham Oscar A. Aguilar Miho Tanaka Richard W. Childs Cynthia E. Dunbar Jack L. Strominger Hernan D. Kopcow James R. Carlyle
author_facet	David S. J. Allan Ana Sofia Cerdeira Anuisa Ranjan Christina L. Kirkham Oscar A. Aguilar Miho Tanaka Richard W. Childs Cynthia E. Dunbar Jack L. Strominger Hernan D. Kopcow James R. Carlyle
author_sort	David S. J. Allan
title	Transcriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells
title_short	Transcriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells
title_full	Transcriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells
title_fullStr	Transcriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells
title_full_unstemmed	Transcriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells
title_sort	transcriptome analysis reveals similarities between human blood cd3− cd56bright cells and mouse cd127+ innate lymphoid cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fdd8503caef444f796bf5bf324863b3b
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Transcriptome analysis reveals similarities between human blood CD3− CD56bright cells and mouse CD127+ innate lymphoid cells

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