Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.

<h4>Objective</h4>Hyperthermia uses exogenous heat induction as a cancer therapy. This work addresses the acute and long-term effects of hyperthermia in the highly metastatic melanoma cell line B16-F10.<h4>Materials and methods</h4>Melanoma cells were submitted to one heat tr...

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Autores principales:	Mónica Pereira Garcia, José Roberto Tinoco Cavalheiro, Maria Helena Fernandes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdd89818140640f0a28a8f91542f129c
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spelling	oai:doaj.org-article:fdd89818140640f0a28a8f91542f129c2021-11-18T07:21:31ZAcute and long-term effects of hyperthermia in B16-F10 melanoma cells.1932-620310.1371/journal.pone.0035489https://doaj.org/article/fdd89818140640f0a28a8f91542f129c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22532856/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>Hyperthermia uses exogenous heat induction as a cancer therapy. This work addresses the acute and long-term effects of hyperthermia in the highly metastatic melanoma cell line B16-F10.<h4>Materials and methods</h4>Melanoma cells were submitted to one heat treatment, 45°C for 30 min, and thereafter were kept at 37°C for an additional period of 14 days. Cultures maintained at 37°C were used as control. Cultures were assessed for the heat shock reaction.<h4>Results</h4>Immediately after the heat shock, cells began a process of fast degradation, and, in the first 24 h, cultures showed decreased viability, alterations in cell morphology and F-actin cytoskeleton organization, significant reduction in the number of adherent cells, most of them in a process of late apoptosis, and an altered gene expression profile. A follow-up of two weeks after heat exposure showed that viability and number of adherent cells remained very low, with a high percentage of early apoptotic cells. Still, heat-treated cultures maintained a low but relatively constant population of cells in S and G(2)/M phases for a long period after heat exposure, evidencing the presence of metabolically active cells.<h4>Conclusion</h4>The melanoma cell line B16-F10 is susceptible to one hyperthermia treatment at 45°C, with significant induced acute and long-term effects. However, a low but apparently stable percentage of metabolically active cells survived long after heat exposure.Mónica Pereira GarciaJosé Roberto Tinoco CavalheiroMaria Helena FernandesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35489 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mónica Pereira Garcia José Roberto Tinoco Cavalheiro Maria Helena Fernandes Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.
description	<h4>Objective</h4>Hyperthermia uses exogenous heat induction as a cancer therapy. This work addresses the acute and long-term effects of hyperthermia in the highly metastatic melanoma cell line B16-F10.<h4>Materials and methods</h4>Melanoma cells were submitted to one heat treatment, 45°C for 30 min, and thereafter were kept at 37°C for an additional period of 14 days. Cultures maintained at 37°C were used as control. Cultures were assessed for the heat shock reaction.<h4>Results</h4>Immediately after the heat shock, cells began a process of fast degradation, and, in the first 24 h, cultures showed decreased viability, alterations in cell morphology and F-actin cytoskeleton organization, significant reduction in the number of adherent cells, most of them in a process of late apoptosis, and an altered gene expression profile. A follow-up of two weeks after heat exposure showed that viability and number of adherent cells remained very low, with a high percentage of early apoptotic cells. Still, heat-treated cultures maintained a low but relatively constant population of cells in S and G(2)/M phases for a long period after heat exposure, evidencing the presence of metabolically active cells.<h4>Conclusion</h4>The melanoma cell line B16-F10 is susceptible to one hyperthermia treatment at 45°C, with significant induced acute and long-term effects. However, a low but apparently stable percentage of metabolically active cells survived long after heat exposure.
format	article
author	Mónica Pereira Garcia José Roberto Tinoco Cavalheiro Maria Helena Fernandes
author_facet	Mónica Pereira Garcia José Roberto Tinoco Cavalheiro Maria Helena Fernandes
author_sort	Mónica Pereira Garcia
title	Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.
title_short	Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.
title_full	Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.
title_fullStr	Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.
title_full_unstemmed	Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.
title_sort	acute and long-term effects of hyperthermia in b16-f10 melanoma cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/fdd89818140640f0a28a8f91542f129c
work_keys_str_mv	AT monicapereiragarcia acuteandlongtermeffectsofhyperthermiainb16f10melanomacells AT joserobertotinococavalheiro acuteandlongtermeffectsofhyperthermiainb16f10melanomacells AT mariahelenafernandes acuteandlongtermeffectsofhyperthermiainb16f10melanomacells
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Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.

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