Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.

Extreme environments Morrope and Bayovar Salt lagoons, several ecosystems and microhabitats remain unexplored, and little is known about the diversity of Actinobacteria. We suggest that the endemic bacteria present in this extreme environment is a source of active molecules with anticancer, antimicr...

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Autores principales:	Rene Flores Clavo, Nataly Ruiz Quiñones, Álvaro Jose Hernández-Tasco, Marcos José Salvador, Ana Lúcia Tasca Gois Ruiz, Lúcia Elaine de Oliveira Braga, Jonas Henrique Costa, Taícia Pacheco Fill, Zhandra Lizeth Arce Gil, Luis Miguel Serquen Lopez, Fabiana Fantinatti Garboggini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdddaff33ddf4f419b8528b6303412e1
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spelling	oai:doaj.org-article:fdddaff33ddf4f419b8528b6303412e12021-12-02T20:06:21ZEvaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.1932-620310.1371/journal.pone.0240946https://doaj.org/article/fdddaff33ddf4f419b8528b6303412e12021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0240946https://doaj.org/toc/1932-6203Extreme environments Morrope and Bayovar Salt lagoons, several ecosystems and microhabitats remain unexplored, and little is known about the diversity of Actinobacteria. We suggest that the endemic bacteria present in this extreme environment is a source of active molecules with anticancer, antimicrobial, and antiparasitic properties. Using phenotypic and genotypic characterization techniques, including 16S rRNA sequencing, we identified these bacteria as members of the genera Streptomyces, Pseudonocardia, Staphylococcus, Bacillus, and Pseudomonas. Actinobacteria strains were found predominantly. Phylogenetic analysis revealed 13 Actinobacteria clusters of Streptomyces, the main genus. Three Streptomycetes, strains MW562814, MW562805, and MW562807 showed antiproliferative activities against three tumor cell lines: U251 glioma, MCF7 breast, and NCI-H460 lung (non-small cell type); and antibacterial activity against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, and the multidrug resistant Acinetobacter baumannii AC-972. The antiproliferative activities (measured as total growth inhibition [TGI]) of Streptomyces sp. MW562807 were 0.57 μg/mL, for 0.61 μg/mL, and 0.80 μg/mL for glioma, lung non-small cell type, and breast cancer cell lines, respectively; the methanolic fraction of the crude extract showed a better antiproliferative activity and could inhibit the growth of (U251 (TGI = 38.3 μg/mL), OVCAR-03 (TGI = 62.1 μg/mL), and K562 (TGI = 81.5 μg/mL)) of nine tumor cells types and one nontumor cell type. Extreme enviroments, such as the Morrope and Bayovar Salt saloons are promising sources of new bacteria, whose compounds may be useful for treating various infectious diseases or even some types of cancer.Rene Flores ClavoNataly Ruiz QuiñonesÁlvaro Jose Hernández-TascoMarcos José SalvadorAna Lúcia Tasca Gois RuizLúcia Elaine de Oliveira BragaJonas Henrique CostaTaícia Pacheco FillZhandra Lizeth Arce GilLuis Miguel Serquen LopezFabiana Fantinatti GarbogginiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0240946 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rene Flores Clavo Nataly Ruiz Quiñones Álvaro Jose Hernández-Tasco Marcos José Salvador Ana Lúcia Tasca Gois Ruiz Lúcia Elaine de Oliveira Braga Jonas Henrique Costa Taícia Pacheco Fill Zhandra Lizeth Arce Gil Luis Miguel Serquen Lopez Fabiana Fantinatti Garboggini Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.
description	Extreme environments Morrope and Bayovar Salt lagoons, several ecosystems and microhabitats remain unexplored, and little is known about the diversity of Actinobacteria. We suggest that the endemic bacteria present in this extreme environment is a source of active molecules with anticancer, antimicrobial, and antiparasitic properties. Using phenotypic and genotypic characterization techniques, including 16S rRNA sequencing, we identified these bacteria as members of the genera Streptomyces, Pseudonocardia, Staphylococcus, Bacillus, and Pseudomonas. Actinobacteria strains were found predominantly. Phylogenetic analysis revealed 13 Actinobacteria clusters of Streptomyces, the main genus. Three Streptomycetes, strains MW562814, MW562805, and MW562807 showed antiproliferative activities against three tumor cell lines: U251 glioma, MCF7 breast, and NCI-H460 lung (non-small cell type); and antibacterial activity against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, and the multidrug resistant Acinetobacter baumannii AC-972. The antiproliferative activities (measured as total growth inhibition [TGI]) of Streptomyces sp. MW562807 were 0.57 μg/mL, for 0.61 μg/mL, and 0.80 μg/mL for glioma, lung non-small cell type, and breast cancer cell lines, respectively; the methanolic fraction of the crude extract showed a better antiproliferative activity and could inhibit the growth of (U251 (TGI = 38.3 μg/mL), OVCAR-03 (TGI = 62.1 μg/mL), and K562 (TGI = 81.5 μg/mL)) of nine tumor cells types and one nontumor cell type. Extreme enviroments, such as the Morrope and Bayovar Salt saloons are promising sources of new bacteria, whose compounds may be useful for treating various infectious diseases or even some types of cancer.
format	article
author	Rene Flores Clavo Nataly Ruiz Quiñones Álvaro Jose Hernández-Tasco Marcos José Salvador Ana Lúcia Tasca Gois Ruiz Lúcia Elaine de Oliveira Braga Jonas Henrique Costa Taícia Pacheco Fill Zhandra Lizeth Arce Gil Luis Miguel Serquen Lopez Fabiana Fantinatti Garboggini
author_facet	Rene Flores Clavo Nataly Ruiz Quiñones Álvaro Jose Hernández-Tasco Marcos José Salvador Ana Lúcia Tasca Gois Ruiz Lúcia Elaine de Oliveira Braga Jonas Henrique Costa Taícia Pacheco Fill Zhandra Lizeth Arce Gil Luis Miguel Serquen Lopez Fabiana Fantinatti Garboggini
author_sort	Rene Flores Clavo
title	Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.
title_short	Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.
title_full	Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.
title_fullStr	Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.
title_full_unstemmed	Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.
title_sort	evaluation of antimicrobial and antiproliferative activities of actinobacteria isolated from the saline lagoons of northwestern peru.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/fdddaff33ddf4f419b8528b6303412e1
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Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.

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