Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>
<i>Homeobox</i> (HB) genes play critical roles in the regulation of plant morphogenesis, growth and development. Here, we identified a total of 156 <i>PtrHB</i> genes from the <i>Populus trichocarpa</i> genome. According to the topologies and taxonomy of the phylo...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/fde18e9ca17b4baeafba0438dcbe8fc1 |
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| Sumario: | <i>Homeobox</i> (HB) genes play critical roles in the regulation of plant morphogenesis, growth and development. Here, we identified a total of 156 <i>PtrHB</i> genes from the <i>Populus trichocarpa</i> genome. According to the topologies and taxonomy of the phylogenetic tree constructed by <i>Arabidopsis thaliana</i> HB members, all PtrHB proteins were divided into six subgroups, namely HD-ZIP, ZF-HD, HB-PHD, TALE, WOX and HB-OTHERS. Multiple alignments of conserved homeodomains (HDs) revealed the conserved loci of each subgroup, while gene structure analysis showed similar exon–intron gene structures, and motif analysis indicated the similarity of motif number and pattern in the same subgroup. Promoter analysis indicated that the promoters of <i>PtrHB</i> genes contain a series of <i>cis</i>-acting regulatory elements involved in responding to various abiotic stresses, indicating that <i>PtrHB</i>s had potential functions in these processes. Collinearity analysis revealed that there are 96 pairs of 127 <i>PtrHB</i> genes mainly distributing on Chromosomes 1, 2, and 5. We analyzed the spatio-temporal expression patterns of <i>PtrHB</i> genes, and the virus-induced gene silencing (VIGS) of <i>PtrHB3</i> gene resulted in the compromised tolerance of poplar seedlings to mannitol treatment. The bioinformatics on <i>PtrHB</i> family and preliminary exploration of drought-responsive genes can provide support for further study of the family in woody plants, especially in drought-related biological processes. It also provides a direction for developing new varieties of poplar with drought resistance. Overall, our results provided significant information for further functional analysis of <i>PtrHB</i> genes in poplar and demonstrated that <i>PtrHB3</i> is a dominant gene regulating tolerance to water stress treatment in poplar seedlings. |
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