Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>

Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>

<i>Homeobox</i> (HB) genes play critical roles in the regulation of plant morphogenesis, growth and development. Here, we identified a total of 156 <i>PtrHB</i> genes from the <i>Populus trichocarpa</i> genome. According to the topologies and taxonomy of the phylo...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Jing Hou, Yan Sun, Lei Wang, Yuanzhong Jiang, Ningning Chen, Shaofei Tong
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Homeobox</i>
phylogenetic analysis
VIGS
<i>PtrHB3</i>
<i>Populus</i>
Botany
QK1-989
Acceso en línea:https://doaj.org/article/fde18e9ca17b4baeafba0438dcbe8fc1
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:fde18e9ca17b4baeafba0438dcbe8fc1
record_format dspace
spelling oai:doaj.org-article:fde18e9ca17b4baeafba0438dcbe8fc12021-11-25T18:44:51ZGenome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>10.3390/plants101122842223-7747https://doaj.org/article/fde18e9ca17b4baeafba0438dcbe8fc12021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2284https://doaj.org/toc/2223-7747<i>Homeobox</i> (HB) genes play critical roles in the regulation of plant morphogenesis, growth and development. Here, we identified a total of 156 <i>PtrHB</i> genes from the <i>Populus trichocarpa</i> genome. According to the topologies and taxonomy of the phylogenetic tree constructed by <i>Arabidopsis thaliana</i> HB members, all PtrHB proteins were divided into six subgroups, namely HD-ZIP, ZF-HD, HB-PHD, TALE, WOX and HB-OTHERS. Multiple alignments of conserved homeodomains (HDs) revealed the conserved loci of each subgroup, while gene structure analysis showed similar exon–intron gene structures, and motif analysis indicated the similarity of motif number and pattern in the same subgroup. Promoter analysis indicated that the promoters of <i>PtrHB</i> genes contain a series of <i>cis</i>-acting regulatory elements involved in responding to various abiotic stresses, indicating that <i>PtrHB</i>s had potential functions in these processes. Collinearity analysis revealed that there are 96 pairs of 127 <i>PtrHB</i> genes mainly distributing on Chromosomes 1, 2, and 5. We analyzed the spatio-temporal expression patterns of <i>PtrHB</i> genes, and the virus-induced gene silencing (VIGS) of <i>PtrHB3</i> gene resulted in the compromised tolerance of poplar seedlings to mannitol treatment. The bioinformatics on <i>PtrHB</i> family and preliminary exploration of drought-responsive genes can provide support for further study of the family in woody plants, especially in drought-related biological processes. It also provides a direction for developing new varieties of poplar with drought resistance. Overall, our results provided significant information for further functional analysis of <i>PtrHB</i> genes in poplar and demonstrated that <i>PtrHB3</i> is a dominant gene regulating tolerance to water stress treatment in poplar seedlings.Jing HouYan SunLei WangYuanzhong JiangNingning ChenShaofei TongMDPI AGarticle<i>Homeobox</i>phylogenetic analysisVIGS<i>PtrHB3</i><i>Populus</i>BotanyQK1-989ENPlants, Vol 10, Iss 2284, p 2284 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Homeobox</i>
phylogenetic analysis
VIGS
<i>PtrHB3</i>
<i>Populus</i>
Botany
QK1-989
spellingShingle <i>Homeobox</i>
phylogenetic analysis
VIGS
<i>PtrHB3</i>
<i>Populus</i>
Botany
QK1-989
Jing Hou
Yan Sun
Lei Wang
Yuanzhong Jiang
Ningning Chen
Shaofei Tong
Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>
description <i>Homeobox</i> (HB) genes play critical roles in the regulation of plant morphogenesis, growth and development. Here, we identified a total of 156 <i>PtrHB</i> genes from the <i>Populus trichocarpa</i> genome. According to the topologies and taxonomy of the phylogenetic tree constructed by <i>Arabidopsis thaliana</i> HB members, all PtrHB proteins were divided into six subgroups, namely HD-ZIP, ZF-HD, HB-PHD, TALE, WOX and HB-OTHERS. Multiple alignments of conserved homeodomains (HDs) revealed the conserved loci of each subgroup, while gene structure analysis showed similar exon–intron gene structures, and motif analysis indicated the similarity of motif number and pattern in the same subgroup. Promoter analysis indicated that the promoters of <i>PtrHB</i> genes contain a series of <i>cis</i>-acting regulatory elements involved in responding to various abiotic stresses, indicating that <i>PtrHB</i>s had potential functions in these processes. Collinearity analysis revealed that there are 96 pairs of 127 <i>PtrHB</i> genes mainly distributing on Chromosomes 1, 2, and 5. We analyzed the spatio-temporal expression patterns of <i>PtrHB</i> genes, and the virus-induced gene silencing (VIGS) of <i>PtrHB3</i> gene resulted in the compromised tolerance of poplar seedlings to mannitol treatment. The bioinformatics on <i>PtrHB</i> family and preliminary exploration of drought-responsive genes can provide support for further study of the family in woody plants, especially in drought-related biological processes. It also provides a direction for developing new varieties of poplar with drought resistance. Overall, our results provided significant information for further functional analysis of <i>PtrHB</i> genes in poplar and demonstrated that <i>PtrHB3</i> is a dominant gene regulating tolerance to water stress treatment in poplar seedlings.
format article
author Jing Hou
Yan Sun
Lei Wang
Yuanzhong Jiang
Ningning Chen
Shaofei Tong
author_facet Jing Hou
Yan Sun
Lei Wang
Yuanzhong Jiang
Ningning Chen
Shaofei Tong
author_sort Jing Hou
title Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>
title_short Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>
title_full Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>
title_fullStr Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>
title_full_unstemmed Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in <i>Populus trichocarpa</i>
title_sort genome-wide analysis of the homeobox gene family and identification of drought-responsive members in <i>populus trichocarpa</i>
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/fde18e9ca17b4baeafba0438dcbe8fc1
work_keys_str_mv AT jinghou genomewideanalysisofthehomeoboxgenefamilyandidentificationofdroughtresponsivemembersinipopulustrichocarpai
AT yansun genomewideanalysisofthehomeoboxgenefamilyandidentificationofdroughtresponsivemembersinipopulustrichocarpai
AT leiwang genomewideanalysisofthehomeoboxgenefamilyandidentificationofdroughtresponsivemembersinipopulustrichocarpai
AT yuanzhongjiang genomewideanalysisofthehomeoboxgenefamilyandidentificationofdroughtresponsivemembersinipopulustrichocarpai
AT ningningchen genomewideanalysisofthehomeoboxgenefamilyandidentificationofdroughtresponsivemembersinipopulustrichocarpai
AT shaofeitong genomewideanalysisofthehomeoboxgenefamilyandidentificationofdroughtresponsivemembersinipopulustrichocarpai
_version_ 1718410811511144448

Ejemplares similares