La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice

Lu Yuan,1 Qingzhao Li,1 Disi Bai,2 Xueliang Shang,2 Fen Hu,3 Zhenfei Chen,4 Tianyang An,5 Yajing Chen,6 Xiujun Zhang1 1College of Public Health; 2College of Psychology; 3College of Life Sciences; 4Environmental Monitoring Center Tang Shan, Tangshan 063210, Hebei, People’s Republic of China...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuan L, Li Q, Bai D, Shang X, Hu F, Chen Z, An T, Chen Y, Zhang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	la2o3 nanoparticles reproductive toxicity inflammation nrf-2/are signaling pathway apotosis. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fde50230f0d043b9bcd03dda9f60e77b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fde50230f0d043b9bcd03dda9f60e77b
record_format	dspace
spelling	oai:doaj.org-article:fde50230f0d043b9bcd03dda9f60e77b2021-12-02T09:43:39ZLa2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice1178-2013https://doaj.org/article/fde50230f0d043b9bcd03dda9f60e77b2020-05-01T00:00:00Zhttps://www.dovepress.com/la2o3-nanoparticles-induce-reproductive-toxicity-mediated-by-the-nrf-2-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lu Yuan,1 Qingzhao Li,1 Disi Bai,2 Xueliang Shang,2 Fen Hu,3 Zhenfei Chen,4 Tianyang An,5 Yajing Chen,6 Xiujun Zhang1 1College of Public Health; 2College of Psychology; 3College of Life Sciences; 4Environmental Monitoring Center Tang Shan, Tangshan 063210, Hebei, People’s Republic of China; 5College of Ji Tang; 6College of Pharmacy of North China University of Science and Technology, Tangshan 063210, Hebei, People’s Republic of ChinaCorrespondence: Xiujun ZhangNorth China University of Science and Technology, Bohai Avenue 21, Tangshan 063210, Hebei, People’s Republic of ChinaTel +86-315-3726335Fax +86-315-3726341Email xiujunzhang66@126.comDisi BaiCollege of Psychology, North China University of Science and Technology, Bohai Avenue 21, Tangshan 063000, People’s Republic of ChinaEmail baidisi@163.comPurpose: Lanthanum oxide (La2O3) nanoparticles (NPs) have been widely used in catalytic and photoelectric applications, but the reproductive toxicity is still unclear. This study evaluated the reproductive toxicity of two different-sized La2O3 particles in the testes.Materials and Methods: Fifty Kunming mice were randomly divided into five groups. Mice were treated with La2O3 NPs by repeated intragastric administration for 90 days (control, nano-sized with 5, 10, 50 mg/kg BW and micro-sized with 50 mg/kg BW). Mice in the control group were treated with de-ionised water without La2O3 NPs. Sperm parameters, testicular histopathology, TEM assessment, hormone assay and nuclear factor erythroid 2-related factor 2 (Nrf-2) pathway were performed and evaluated.Results: The body weight of mice treated with La2O3 NPs or not had no difference; sperm parameters and histological assessment showed that La2O3 NPs could induce reproductive toxicity in the testicle. Serum testosterone and gonadotropin-releasing hormone (GnRH) in the NH (nano-sized with 50 mg/kg BW) group were markedly decreased relative to control group, and an increase of luteinizing hormone (LH) in NH group was detected . Additionally, transmission electron microscopy revealed that the ultrastructural abnormalities induced by La2O3 NPs were more severe than La2O3 MPs in the testes. Furthermore, La2O3 NPs treatment inhibited the translocation of nuclear factor erythroid 2-related factor 2 (Nrf-2) from the cytoplasm into the nucleus as well as the expression of downstream genes NAD(P)H quinone oxidoreductase1 (NQO1), hemeoxygenase 1 (HO-1) and (glutathione peroxidase) GSH-Px, thus abrogating Nrf-2-mediated defense mechanisms against oxidative stress.Conclusions: The results of this study demonstrated that La2O3 NPs improved the spermatogenesis defects in mice. La2O3 NPs inhibited Nrf-2/ARE signaling pathway that resulted in apoptosis in the mice testes.Keywords: La2O3 nanoparticles, reproductive toxicity, inflammation, Nrf-2/ARE signaling pathway, apoptosisYuan LLi QBai DShang XHu FChen ZAn TChen YZhang XDove Medical Pressarticlela2o3 nanoparticlesreproductive toxicityinflammationnrf-2/are signaling pathwayapotosis.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3415-3431 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	la2o3 nanoparticles reproductive toxicity inflammation nrf-2/are signaling pathway apotosis. Medicine (General) R5-920
spellingShingle	la2o3 nanoparticles reproductive toxicity inflammation nrf-2/are signaling pathway apotosis. Medicine (General) R5-920 Yuan L Li Q Bai D Shang X Hu F Chen Z An T Chen Y Zhang X La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice
description	Lu Yuan,1 Qingzhao Li,1 Disi Bai,2 Xueliang Shang,2 Fen Hu,3 Zhenfei Chen,4 Tianyang An,5 Yajing Chen,6 Xiujun Zhang1 1College of Public Health; 2College of Psychology; 3College of Life Sciences; 4Environmental Monitoring Center Tang Shan, Tangshan 063210, Hebei, People’s Republic of China; 5College of Ji Tang; 6College of Pharmacy of North China University of Science and Technology, Tangshan 063210, Hebei, People’s Republic of ChinaCorrespondence: Xiujun ZhangNorth China University of Science and Technology, Bohai Avenue 21, Tangshan 063210, Hebei, People’s Republic of ChinaTel +86-315-3726335Fax +86-315-3726341Email xiujunzhang66@126.comDisi BaiCollege of Psychology, North China University of Science and Technology, Bohai Avenue 21, Tangshan 063000, People’s Republic of ChinaEmail baidisi@163.comPurpose: Lanthanum oxide (La2O3) nanoparticles (NPs) have been widely used in catalytic and photoelectric applications, but the reproductive toxicity is still unclear. This study evaluated the reproductive toxicity of two different-sized La2O3 particles in the testes.Materials and Methods: Fifty Kunming mice were randomly divided into five groups. Mice were treated with La2O3 NPs by repeated intragastric administration for 90 days (control, nano-sized with 5, 10, 50 mg/kg BW and micro-sized with 50 mg/kg BW). Mice in the control group were treated with de-ionised water without La2O3 NPs. Sperm parameters, testicular histopathology, TEM assessment, hormone assay and nuclear factor erythroid 2-related factor 2 (Nrf-2) pathway were performed and evaluated.Results: The body weight of mice treated with La2O3 NPs or not had no difference; sperm parameters and histological assessment showed that La2O3 NPs could induce reproductive toxicity in the testicle. Serum testosterone and gonadotropin-releasing hormone (GnRH) in the NH (nano-sized with 50 mg/kg BW) group were markedly decreased relative to control group, and an increase of luteinizing hormone (LH) in NH group was detected . Additionally, transmission electron microscopy revealed that the ultrastructural abnormalities induced by La2O3 NPs were more severe than La2O3 MPs in the testes. Furthermore, La2O3 NPs treatment inhibited the translocation of nuclear factor erythroid 2-related factor 2 (Nrf-2) from the cytoplasm into the nucleus as well as the expression of downstream genes NAD(P)H quinone oxidoreductase1 (NQO1), hemeoxygenase 1 (HO-1) and (glutathione peroxidase) GSH-Px, thus abrogating Nrf-2-mediated defense mechanisms against oxidative stress.Conclusions: The results of this study demonstrated that La2O3 NPs improved the spermatogenesis defects in mice. La2O3 NPs inhibited Nrf-2/ARE signaling pathway that resulted in apoptosis in the mice testes.Keywords: La2O3 nanoparticles, reproductive toxicity, inflammation, Nrf-2/ARE signaling pathway, apoptosis
format	article
author	Yuan L Li Q Bai D Shang X Hu F Chen Z An T Chen Y Zhang X
author_facet	Yuan L Li Q Bai D Shang X Hu F Chen Z An T Chen Y Zhang X
author_sort	Yuan L
title	La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice
title_short	La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice
title_full	La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice
title_fullStr	La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice
title_full_unstemmed	La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice
title_sort	la2o3 nanoparticles induce reproductive toxicity mediated by the nrf-2/are signaling pathway in kunming mice
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/fde50230f0d043b9bcd03dda9f60e77b
work_keys_str_mv	AT yuanl la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice AT liq la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice AT baid la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice AT shangx la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice AT huf la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice AT chenz la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice AT ant la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice AT cheny la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice AT zhangx la2o3nanoparticlesinducereproductivetoxicitymediatedbythenrf2aresignalingpathwayinkunmingmice
_version_	1718398042934083584

La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice

Ejemplares similares