Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)

Gender identification of fish species is carried out mainly by examining external morphological characteristics, which in general, it is very complex and not always a reliable approach. Electrophoresis of plasma proteins can be used as an alternative and useful molecular tool for a more precise sex...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Popovski Zoran, Kwasek Karolina, Wojno Michal, Dabrowski Konrad, Wick Macdonald
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	koi carp sds-page apolipoprotein structure Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/fdef6f04e92b406d969b9fd7fd560837
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fdef6f04e92b406d969b9fd7fd560837
record_format	dspace
spelling	oai:doaj.org-article:fdef6f04e92b406d969b9fd7fd5608372021-11-17T21:27:51ZIdentification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)1820-744810.1515/acve-2017-0023https://doaj.org/article/fdef6f04e92b406d969b9fd7fd5608372017-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0023https://doaj.org/toc/1820-7448Gender identification of fish species is carried out mainly by examining external morphological characteristics, which in general, it is very complex and not always a reliable approach. Electrophoresis of plasma proteins can be used as an alternative and useful molecular tool for a more precise sex determination. The presence of female specific proteins in the plasma is a starting point for the application of this technique. In this study, reducing discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze plasma proteins of male and female koi carp (Cyprinus carpio haematopterus). Image analyses of electrophoregrams with resolved plasma proteins by SDS-PAGE showed that it is an appropriate technique to discriminate male from female samples. It is based on the presence of apolipoprotein B-100 which can be used as a suitable marker. Further amino acid characterization of apolipoprotein B-100 confirmed that it is a specific protein for female individuals.Popovski ZoranKwasek KarolinaWojno MichalDabrowski KonradWick MacdonaldSciendoarticlekoi carpsds-pageapolipoproteinstructureVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 2, Pp 285-291 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	koi carp sds-page apolipoprotein structure Veterinary medicine SF600-1100
spellingShingle	koi carp sds-page apolipoprotein structure Veterinary medicine SF600-1100 Popovski Zoran Kwasek Karolina Wojno Michal Dabrowski Konrad Wick Macdonald Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)
description	Gender identification of fish species is carried out mainly by examining external morphological characteristics, which in general, it is very complex and not always a reliable approach. Electrophoresis of plasma proteins can be used as an alternative and useful molecular tool for a more precise sex determination. The presence of female specific proteins in the plasma is a starting point for the application of this technique. In this study, reducing discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze plasma proteins of male and female koi carp (Cyprinus carpio haematopterus). Image analyses of electrophoregrams with resolved plasma proteins by SDS-PAGE showed that it is an appropriate technique to discriminate male from female samples. It is based on the presence of apolipoprotein B-100 which can be used as a suitable marker. Further amino acid characterization of apolipoprotein B-100 confirmed that it is a specific protein for female individuals.
format	article
author	Popovski Zoran Kwasek Karolina Wojno Michal Dabrowski Konrad Wick Macdonald
author_facet	Popovski Zoran Kwasek Karolina Wojno Michal Dabrowski Konrad Wick Macdonald
author_sort	Popovski Zoran
title	Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)
title_short	Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)
title_full	Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)
title_fullStr	Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)
title_full_unstemmed	Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)
title_sort	identification and partial characterization of a sex specific protein in koi carp (cyprinus carpio haematopterus)
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/fdef6f04e92b406d969b9fd7fd560837
work_keys_str_mv	AT popovskizoran identificationandpartialcharacterizationofasexspecificproteininkoicarpcyprinuscarpiohaematopterus AT kwasekkarolina identificationandpartialcharacterizationofasexspecificproteininkoicarpcyprinuscarpiohaematopterus AT wojnomichal identificationandpartialcharacterizationofasexspecificproteininkoicarpcyprinuscarpiohaematopterus AT dabrowskikonrad identificationandpartialcharacterizationofasexspecificproteininkoicarpcyprinuscarpiohaematopterus AT wickmacdonald identificationandpartialcharacterizationofasexspecificproteininkoicarpcyprinuscarpiohaematopterus
_version_	1718425330143723520

Identification and partial characterization of a sex specific protein in Koi carp (Cyprinus carpio haematopterus)

Ejemplares similares